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Cellular thermal shift assay for the identification of drug-target interactions in the Plasmodium falciparum proteome.

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Title: Cellular thermal shift assay for the identification of drug-target interactions in the Plasmodium falciparum proteome.
Authors: Dziekan JM; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore., Wirjanata G; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore., Dai L; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.; The Second Clinical Medical College of Jinan University, First Affiliated Hospital of Southern University of Science and Technology, Shenzhen People's Hospital, Shenzhen, China., Go KD; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore., Yu H; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore., Lim YT; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.; Functional Proteomics Laboratory, Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore., Chen L; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.; Functional Proteomics Laboratory, Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore., Wang LC; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.; Functional Proteomics Laboratory, Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore., Puspita B; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore., Prabhu N; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore., Sobota RM; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore. rmsobota@imcb.a-star.edu.sg.; Functional Proteomics Laboratory, Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore. rmsobota@imcb.a-star.edu.sg., Nordlund P; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore. pnordlund@ntu.edu.sg.; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore. pnordlund@ntu.edu.sg.; Department of Oncology and Pathology, Karolinska Institutet, Stockholm, Sweden. pnordlund@ntu.edu.sg., Bozdech Z; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore. zbozdech@ntu.edu.sg.
Source: Nature protocols [Nat Protoc] 2020 Jun; Vol. 15 (6), pp. 1881-1921. Date of Electronic Publication: 2020 Apr 27.
Publication Type: Journal Article; Research Support, Non-U.S. Gov't
Journal Info: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101284307 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1750-2799 (Electronic) Linking ISSN: 17502799 NLM ISO Abbreviation: Nat Protoc Subsets: MEDLINE
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  Data: <searchLink fieldCode="AU" term="%22Dziekan+JM%22">Dziekan JM</searchLink>; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.<br /><searchLink fieldCode="AU" term="%22Wirjanata+G%22">Wirjanata G</searchLink>; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.<br /><searchLink fieldCode="AU" term="%22Dai+L%22">Dai L</searchLink>; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.; The Second Clinical Medical College of Jinan University, First Affiliated Hospital of Southern University of Science and Technology, Shenzhen People's Hospital, Shenzhen, China.<br /><searchLink fieldCode="AU" term="%22Go+KD%22">Go KD</searchLink>; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.<br /><searchLink fieldCode="AU" term="%22Yu+H%22">Yu H</searchLink>; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.<br /><searchLink fieldCode="AU" term="%22Lim+YT%22">Lim YT</searchLink>; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.; Functional Proteomics Laboratory, Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.<br /><searchLink fieldCode="AU" term="%22Chen+L%22">Chen L</searchLink>; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.; Functional Proteomics Laboratory, Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.<br /><searchLink fieldCode="AU" term="%22Wang+LC%22">Wang LC</searchLink>; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.; Functional Proteomics Laboratory, Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.<br /><searchLink fieldCode="AU" term="%22Puspita+B%22">Puspita B</searchLink>; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.<br /><searchLink fieldCode="AU" term="%22Prabhu+N%22">Prabhu N</searchLink>; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.<br /><searchLink fieldCode="AU" term="%22Sobota+RM%22">Sobota RM</searchLink>; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore. rmsobota@imcb.a-star.edu.sg.; Functional Proteomics Laboratory, Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore. rmsobota@imcb.a-star.edu.sg.<br /><searchLink fieldCode="AU" term="%22Nordlund+P%22">Nordlund P</searchLink>; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore. pnordlund@ntu.edu.sg.; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore. pnordlund@ntu.edu.sg.; Department of Oncology and Pathology, Karolinska Institutet, Stockholm, Sweden. pnordlund@ntu.edu.sg.<br /><searchLink fieldCode="AU" term="%22Bozdech+Z%22">Bozdech Z</searchLink>; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore. zbozdech@ntu.edu.sg.
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              Text: 2020 Jun
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