In vitro prototyping and rapid optimization of biosynthetic enzymes for cell design.

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Title: In vitro prototyping and rapid optimization of biosynthetic enzymes for cell design.
Authors: Karim AS; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA., Dudley QM; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA., Juminaga A; LanzaTech Inc., Skokie, IL, USA., Yuan Y; LanzaTech Inc., Skokie, IL, USA., Crowe SA; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA., Heggestad JT; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA., Garg S; LanzaTech Inc., Skokie, IL, USA., Abdalla T; LanzaTech Inc., Skokie, IL, USA., Grubbe WS; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA., Rasor BJ; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA., Coar DN; Lockheed Martin Advanced Technology Laboratories, Cherry Hill, NJ, USA., Torculas M; Lockheed Martin Advanced Technology Laboratories, Cherry Hill, NJ, USA., Krein M; Lockheed Martin Advanced Technology Laboratories, Cherry Hill, NJ, USA., Liew FE; LanzaTech Inc., Skokie, IL, USA., Quattlebaum A; LanzaTech Inc., Skokie, IL, USA., Jensen RO; LanzaTech Inc., Skokie, IL, USA., Stuart JA; Lockheed Martin Advanced Technology Laboratories, Cherry Hill, NJ, USA., Simpson SD; LanzaTech Inc., Skokie, IL, USA., Köpke M; LanzaTech Inc., Skokie, IL, USA. Michael.Koepke@lanzatech.com., Jewett MC; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA. m-jewett@northwestern.edu.; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA. m-jewett@northwestern.edu.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA. m-jewett@northwestern.edu.; Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, IL, USA. m-jewett@northwestern.edu.; Simpson Querrey Institute, Northwestern University, Chicago, IL, USA. m-jewett@northwestern.edu.
Source: Nature chemical biology [Nat Chem Biol] 2020 Aug; Vol. 16 (8), pp. 912-919. Date of Electronic Publication: 2020 Jun 15.
Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
Journal Info: Publisher: Nature Pub. Group Country of Publication: United States NLM ID: 101231976 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1552-4469 (Electronic) Linking ISSN: 15524450 NLM ISO Abbreviation: Nat Chem Biol Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:1552-4469
DOI:10.1038/s41589-020-0559-0