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In vitro prototyping and rapid optimization of biosynthetic enzymes for cell design.

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Title: In vitro prototyping and rapid optimization of biosynthetic enzymes for cell design.
Authors: Karim AS; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA., Dudley QM; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA., Juminaga A; LanzaTech Inc., Skokie, IL, USA., Yuan Y; LanzaTech Inc., Skokie, IL, USA., Crowe SA; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA., Heggestad JT; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA., Garg S; LanzaTech Inc., Skokie, IL, USA., Abdalla T; LanzaTech Inc., Skokie, IL, USA., Grubbe WS; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA., Rasor BJ; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA., Coar DN; Lockheed Martin Advanced Technology Laboratories, Cherry Hill, NJ, USA., Torculas M; Lockheed Martin Advanced Technology Laboratories, Cherry Hill, NJ, USA., Krein M; Lockheed Martin Advanced Technology Laboratories, Cherry Hill, NJ, USA., Liew FE; LanzaTech Inc., Skokie, IL, USA., Quattlebaum A; LanzaTech Inc., Skokie, IL, USA., Jensen RO; LanzaTech Inc., Skokie, IL, USA., Stuart JA; Lockheed Martin Advanced Technology Laboratories, Cherry Hill, NJ, USA., Simpson SD; LanzaTech Inc., Skokie, IL, USA., Köpke M; LanzaTech Inc., Skokie, IL, USA. Michael.Koepke@lanzatech.com., Jewett MC; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA. m-jewett@northwestern.edu.; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA. m-jewett@northwestern.edu.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA. m-jewett@northwestern.edu.; Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, IL, USA. m-jewett@northwestern.edu.; Simpson Querrey Institute, Northwestern University, Chicago, IL, USA. m-jewett@northwestern.edu.
Source: Nature chemical biology [Nat Chem Biol] 2020 Aug; Vol. 16 (8), pp. 912-919. Date of Electronic Publication: 2020 Jun 15.
Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
Journal Info: Publisher: Nature Pub. Group Country of Publication: United States NLM ID: 101231976 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1552-4469 (Electronic) Linking ISSN: 15524450 NLM ISO Abbreviation: Nat Chem Biol Subsets: MEDLINE
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  Data: <searchLink fieldCode="AU" term="%22Karim+AS%22">Karim AS</searchLink>; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA.<br /><searchLink fieldCode="AU" term="%22Dudley+QM%22">Dudley QM</searchLink>; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA.<br /><searchLink fieldCode="AU" term="%22Juminaga+A%22">Juminaga A</searchLink>; LanzaTech Inc., Skokie, IL, USA.<br /><searchLink fieldCode="AU" term="%22Yuan+Y%22">Yuan Y</searchLink>; LanzaTech Inc., Skokie, IL, USA.<br /><searchLink fieldCode="AU" term="%22Crowe+SA%22">Crowe SA</searchLink>; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA.<br /><searchLink fieldCode="AU" term="%22Heggestad+JT%22">Heggestad JT</searchLink>; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA.<br /><searchLink fieldCode="AU" term="%22Garg+S%22">Garg S</searchLink>; LanzaTech Inc., Skokie, IL, USA.<br /><searchLink fieldCode="AU" term="%22Abdalla+T%22">Abdalla T</searchLink>; LanzaTech Inc., Skokie, IL, USA.<br /><searchLink fieldCode="AU" term="%22Grubbe+WS%22">Grubbe WS</searchLink>; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA.<br /><searchLink fieldCode="AU" term="%22Rasor+BJ%22">Rasor BJ</searchLink>; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA.<br /><searchLink fieldCode="AU" term="%22Coar+DN%22">Coar DN</searchLink>; Lockheed Martin Advanced Technology Laboratories, Cherry Hill, NJ, USA.<br /><searchLink fieldCode="AU" term="%22Torculas+M%22">Torculas M</searchLink>; Lockheed Martin Advanced Technology Laboratories, Cherry Hill, NJ, USA.<br /><searchLink fieldCode="AU" term="%22Krein+M%22">Krein M</searchLink>; Lockheed Martin Advanced Technology Laboratories, Cherry Hill, NJ, USA.<br /><searchLink fieldCode="AU" term="%22Liew+FE%22">Liew FE</searchLink>; LanzaTech Inc., Skokie, IL, USA.<br /><searchLink fieldCode="AU" term="%22Quattlebaum+A%22">Quattlebaum A</searchLink>; LanzaTech Inc., Skokie, IL, USA.<br /><searchLink fieldCode="AU" term="%22Jensen+RO%22">Jensen RO</searchLink>; LanzaTech Inc., Skokie, IL, USA.<br /><searchLink fieldCode="AU" term="%22Stuart+JA%22">Stuart JA</searchLink>; Lockheed Martin Advanced Technology Laboratories, Cherry Hill, NJ, USA.<br /><searchLink fieldCode="AU" term="%22Simpson+SD%22">Simpson SD</searchLink>; LanzaTech Inc., Skokie, IL, USA.<br /><searchLink fieldCode="AU" term="%22Köpke+M%22">Köpke M</searchLink>; LanzaTech Inc., Skokie, IL, USA. Michael.Koepke@lanzatech.com.<br /><searchLink fieldCode="AU" term="%22Jewett+MC%22">Jewett MC</searchLink>; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA. m-jewett@northwestern.edu.; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA. m-jewett@northwestern.edu.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA. m-jewett@northwestern.edu.; Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, IL, USA. m-jewett@northwestern.edu.; Simpson Querrey Institute, Northwestern University, Chicago, IL, USA. m-jewett@northwestern.edu.
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  Data: <searchLink fieldCode="JN" term="%22101231976%22">Nature chemical biology</searchLink> [Nat Chem Biol] 2020 Aug; Vol. 16 (8), pp. 912-919. <i>Date of Electronic Publication: </i>2020 Jun 15.
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