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Detection and quantification of SARS-CoV-2 by droplet digital PCR in real-time PCR negative nasopharyngeal swabs from suspected COVID-19 patients.

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Title: Detection and quantification of SARS-CoV-2 by droplet digital PCR in real-time PCR negative nasopharyngeal swabs from suspected COVID-19 patients.
Authors: Alteri C; Department of Oncology and Hemato-oncology, University of Milan, Milan, Italy., Cento V; Residency in Microbiology and Virology, Università degli Studi di Milano, Milan, Italy., Antonello M; Department of Oncology and Hemato-oncology, University of Milan, Milan, Italy., Colagrossi L; Department of Laboratories, Bambino Gesù Children's Hospital, Rome, Italy., Merli M; Infectious Diseases, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy., Ughi N; Rheumatology Unit, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy., Renica S; Department of Oncology and Hemato-oncology, University of Milan, Milan, Italy., Matarazzo E; Residency in Microbiology and Virology, Università degli Studi di Milano, Milan, Italy., Di Ruscio F; Residency in Microbiology and Virology, Università degli Studi di Milano, Milan, Italy., Tartaglione L; Residency in Microbiology and Virology, Università degli Studi di Milano, Milan, Italy., Colombo J; Department of Cardiotoracovascular Anesthesia and Intensive Care, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy., Grimaldi C; Department of Laboratory Medicine, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy., Carta S; Department of Laboratory Medicine, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy., Nava A; Department of Laboratory Medicine, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy., Costabile V; Department of Pathophysiology and Transplantation, University of Milan, Milan, Italy., Baiguera C; Infectious Diseases, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy., Campisi D; Department of Laboratory Medicine, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy., Fanti D; Department of Laboratory Medicine, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy., Vismara C; Department of Laboratory Medicine, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy., Fumagalli R; Department of Anesthesiology, Critical Care and Pain Medicine, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy., Scaglione F; Department of Oncology and Hemato-oncology, University of Milan, Milan, Italy., Epis OM; Rheumatology Unit, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy., Puoti M; Infectious Diseases, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy., Perno CF; Department of Oncology and Hemato-oncology, University of Milan, Milan, Italy.; Department of Laboratory Medicine, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.
Source: PloS one [PLoS One] 2020 Sep 08; Vol. 15 (9), pp. e0236311. Date of Electronic Publication: 2020 Sep 08 (Print Publication: 2020).
Publication Type: Journal Article; Research Support, Non-U.S. Gov't
Journal Info: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: Detection and quantification of SARS-CoV-2 by droplet digital PCR in real-time PCR negative nasopharyngeal swabs from suspected COVID-19 patients.
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  Data: <searchLink fieldCode="AU" term="%22Alteri+C%22">Alteri C</searchLink>; Department of Oncology and Hemato-oncology, University of Milan, Milan, Italy.<br /><searchLink fieldCode="AU" term="%22Cento+V%22">Cento V</searchLink>; Residency in Microbiology and Virology, Università degli Studi di Milano, Milan, Italy.<br /><searchLink fieldCode="AU" term="%22Antonello+M%22">Antonello M</searchLink>; Department of Oncology and Hemato-oncology, University of Milan, Milan, Italy.<br /><searchLink fieldCode="AU" term="%22Colagrossi+L%22">Colagrossi L</searchLink>; Department of Laboratories, Bambino Gesù Children's Hospital, Rome, Italy.<br /><searchLink fieldCode="AU" term="%22Merli+M%22">Merli M</searchLink>; Infectious Diseases, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.<br /><searchLink fieldCode="AU" term="%22Ughi+N%22">Ughi N</searchLink>; Rheumatology Unit, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.<br /><searchLink fieldCode="AU" term="%22Renica+S%22">Renica S</searchLink>; Department of Oncology and Hemato-oncology, University of Milan, Milan, Italy.<br /><searchLink fieldCode="AU" term="%22Matarazzo+E%22">Matarazzo E</searchLink>; Residency in Microbiology and Virology, Università degli Studi di Milano, Milan, Italy.<br /><searchLink fieldCode="AU" term="%22Di+Ruscio+F%22">Di Ruscio F</searchLink>; Residency in Microbiology and Virology, Università degli Studi di Milano, Milan, Italy.<br /><searchLink fieldCode="AU" term="%22Tartaglione+L%22">Tartaglione L</searchLink>; Residency in Microbiology and Virology, Università degli Studi di Milano, Milan, Italy.<br /><searchLink fieldCode="AU" term="%22Colombo+J%22">Colombo J</searchLink>; Department of Cardiotoracovascular Anesthesia and Intensive Care, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.<br /><searchLink fieldCode="AU" term="%22Grimaldi+C%22">Grimaldi C</searchLink>; Department of Laboratory Medicine, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.<br /><searchLink fieldCode="AU" term="%22Carta+S%22">Carta S</searchLink>; Department of Laboratory Medicine, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.<br /><searchLink fieldCode="AU" term="%22Nava+A%22">Nava A</searchLink>; Department of Laboratory Medicine, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.<br /><searchLink fieldCode="AU" term="%22Costabile+V%22">Costabile V</searchLink>; Department of Pathophysiology and Transplantation, University of Milan, Milan, Italy.<br /><searchLink fieldCode="AU" term="%22Baiguera+C%22">Baiguera C</searchLink>; Infectious Diseases, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.<br /><searchLink fieldCode="AU" term="%22Campisi+D%22">Campisi D</searchLink>; Department of Laboratory Medicine, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.<br /><searchLink fieldCode="AU" term="%22Fanti+D%22">Fanti D</searchLink>; Department of Laboratory Medicine, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.<br /><searchLink fieldCode="AU" term="%22Vismara+C%22">Vismara C</searchLink>; Department of Laboratory Medicine, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.<br /><searchLink fieldCode="AU" term="%22Fumagalli+R%22">Fumagalli R</searchLink>; Department of Anesthesiology, Critical Care and Pain Medicine, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.<br /><searchLink fieldCode="AU" term="%22Scaglione+F%22">Scaglione F</searchLink>; Department of Oncology and Hemato-oncology, University of Milan, Milan, Italy.<br /><searchLink fieldCode="AU" term="%22Epis+OM%22">Epis OM</searchLink>; Rheumatology Unit, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.<br /><searchLink fieldCode="AU" term="%22Puoti+M%22">Puoti M</searchLink>; Infectious Diseases, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.<br /><searchLink fieldCode="AU" term="%22Perno+CF%22">Perno CF</searchLink>; Department of Oncology and Hemato-oncology, University of Milan, Milan, Italy.; Department of Laboratory Medicine, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.
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  Data: <searchLink fieldCode="JN" term="%22101285081%22">PloS one</searchLink> [PLoS One] 2020 Sep 08; Vol. 15 (9), pp. e0236311. <i>Date of Electronic Publication: </i>2020 Sep 08 (<i>Print Publication: </i>2020).
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