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Massively scaled-up testing for SARS-CoV-2 RNA via next-generation sequencing of pooled and barcoded nasal and saliva samples.

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Title: Massively scaled-up testing for SARS-CoV-2 RNA via next-generation sequencing of pooled and barcoded nasal and saliva samples.
Authors: Bloom JS; Department of Human Genetics, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA. jbloom@mednet.ucla.edu.; Howard Hughes Medical Institute, Chevy Chase, MD, USA. jbloom@mednet.ucla.edu.; Octant Inc., Emeryville, CA, USA. jbloom@mednet.ucla.edu., Sathe L; Department of Pathology & Laboratory Medicine, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA., Munugala C; Department of Human Genetics, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA.; Howard Hughes Medical Institute, Chevy Chase, MD, USA., Jones EM; Octant Inc., Emeryville, CA, USA., Gasperini M; Octant Inc., Emeryville, CA, USA., Lubock NB; Octant Inc., Emeryville, CA, USA., Yarza F; Octant Inc., Emeryville, CA, USA., Thompson EM; Octant Inc., Emeryville, CA, USA., Kovary KM; Octant Inc., Emeryville, CA, USA., Park J; Octant Inc., Emeryville, CA, USA., Marquette D; Department of Computational Medicine, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA., Kay S; Department of Computational Medicine, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA., Lucas M; Department of Computational Medicine, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA., Love T; Department of Computational Medicine, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA., Sina Booeshaghi A; Department of Mechanical Engineering, Caltech, Pasadena, CA, USA., Brandenberg OF; Department of Human Genetics, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA.; Howard Hughes Medical Institute, Chevy Chase, MD, USA.; Department of Biological Chemistry, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA., Guo L; Department of Human Genetics, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA.; Howard Hughes Medical Institute, Chevy Chase, MD, USA.; Department of Biological Chemistry, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA., Boocock J; Department of Human Genetics, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA.; Howard Hughes Medical Institute, Chevy Chase, MD, USA.; Department of Biological Chemistry, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA., Hochman M; Octant Inc., Emeryville, CA, USA., Simpkins SW; Octant Inc., Emeryville, CA, USA., Lin I; Department of Human Genetics, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA.; Department of Pathology & Laboratory Medicine, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA., LaPierre N; Department of Computer Science, Samueli School of Engineering, UCLA, Los Angeles, CA, USA., Hong D; Department of Computational Medicine, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA., Zhang Y; Department of Human Genetics, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA., Oland G; Department of Surgery, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA., Choe BJ; Department of Emergency Medicine, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA., Chandrasekaran S; Department of Pathology & Laboratory Medicine, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA., Hilt EE; Department of Pathology & Laboratory Medicine, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA., Butte MJ; Department of Pediatrics, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA.; Department of Microbiology, Immunology & Molecular Genetics, UCLA, Los Angeles, CA, USA., Damoiseaux R; California NanoSystems Institute, UCLA, Los Angeles, CA, USA.; Department of Bioengineering, Samueli School of Engineering, UCLA, Los Angeles, CA, USA.; Department of Medical and Molecular Pharmacology, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA., Kravit C; Department of Digital Technology, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA., Cooper AR; Octant Inc., Emeryville, CA, USA., Yin Y; Department of Human Genetics, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA., Pachter L; Division of Biology and Bioengineering, Department of Computing and Mathematical Sciences, Caltech, Pasadena, CA, USA., Garner OB; Department of Pathology & Laboratory Medicine, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA., Flint J; Department of Human Genetics, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA.; Department of Psychiatry and Biobehavioral Sciences, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA., Eskin E; Department of Human Genetics, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA.; Department of Computational Medicine, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA.; Department of Computer Science, Samueli School of Engineering, UCLA, Los Angeles, CA, USA., Luo C; Department of Human Genetics, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA., Kosuri S; Octant Inc., Emeryville, CA, USA. sri@octant.bio.; Department of Chemistry and Biochemistry, UCLA, Los Angeles, CA, USA. sri@octant.bio., Kruglyak L; Department of Human Genetics, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA. lkruglyak@mednet.ucla.edu.; Howard Hughes Medical Institute, Chevy Chase, MD, USA. lkruglyak@mednet.ucla.edu.; Department of Biological Chemistry, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA. lkruglyak@mednet.ucla.edu., Arboleda VA; Department of Human Genetics, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA. varboleda@mednet.ucla.edu.; Department of Pathology & Laboratory Medicine, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA. varboleda@mednet.ucla.edu.
Source: Nature biomedical engineering [Nat Biomed Eng] 2021 Jul; Vol. 5 (7), pp. 657-665. Date of Electronic Publication: 2021 Jul 01.
Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
Journal Info: Publisher: Springer Nature Country of Publication: England NLM ID: 101696896 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2157-846X (Electronic) Linking ISSN: 2157846X NLM ISO Abbreviation: Nat Biomed Eng Subsets: MEDLINE
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