Tiled-ClickSeq for targeted sequencing of complete coronavirus genomes with simultaneous capture of RNA recombination and minority variants.

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Title: Tiled-ClickSeq for targeted sequencing of complete coronavirus genomes with simultaneous capture of RNA recombination and minority variants.
Authors: Jaworski E; Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, United States.; ClickSeq Technologies LLC, Galveston, United States., Langsjoen RM; Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, United States., Mitchell B; World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston, United States.; Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, United States., Judy B; Department of Pediatrics, University of Texas Medical Branch, Galveston, United States., Newman P; Department of Pediatrics, University of Texas Medical Branch, Galveston, United States., Plante JA; World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston, United States.; Department of Pathology, University of Texas Medical Branch, Galveston, United States.; Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, United States., Plante KS; World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston, United States.; Department of Pathology, University of Texas Medical Branch, Galveston, United States.; Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, United States., Miller AL; Department of Pediatrics, University of Texas Medical Branch, Galveston, United States., Zhou Y; Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, United States., Swetnam D; Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, United States., Sotcheff S; Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, United States., Morris V; Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, United States., Saada N; World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston, United States.; Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, United States., Machado RR; World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston, United States.; Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, United States., McConnell A; World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston, United States.; Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, United States., Widen SG; Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, United States.; Next-Generation Sequencing Core, The University of Texas Medical Branch, Galveston, United States., Thompson J; Next-Generation Sequencing Core, The University of Texas Medical Branch, Galveston, United States., Dong J; Department of Pediatrics, University of Texas Medical Branch, Galveston, United States.; Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, United States., Ren P; Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, United States., Pyles RB; Department of Pediatrics, University of Texas Medical Branch, Galveston, United States., Ksiazek TG; World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston, United States.; Department of Pathology, University of Texas Medical Branch, Galveston, United States., Menachery VD; World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston, United States.; Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, United States.; Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, United States., Weaver SC; World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston, United States.; Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, United States.; Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, United States., Routh AL; Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, United States.; Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, United States.; Sealy Centre for Structural Biology and Molecular Biophysics, University of Texas Medical Branch, Galveston, United States.
Source: ELife [Elife] 2021 Sep 28; Vol. 10. Date of Electronic Publication: 2021 Sep 28.
Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
Journal Info: Publisher: eLife Sciences Publications, Ltd Country of Publication: England NLM ID: 101579614 Publication Model: Electronic Cited Medium: Internet ISSN: 2050-084X (Electronic) Linking ISSN: 2050084X NLM ISO Abbreviation: Elife Subsets: MEDLINE
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  Data: Tiled-ClickSeq for targeted sequencing of complete coronavirus genomes with simultaneous capture of RNA recombination and minority variants.
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  Data: <searchLink fieldCode="AU" term="%22Jaworski+E%22">Jaworski E</searchLink>; Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, United States.; ClickSeq Technologies LLC, Galveston, United States.<br /><searchLink fieldCode="AU" term="%22Langsjoen+RM%22">Langsjoen RM</searchLink>; Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, United States.<br /><searchLink fieldCode="AU" term="%22Mitchell+B%22">Mitchell B</searchLink>; World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston, United States.; Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, United States.<br /><searchLink fieldCode="AU" term="%22Judy+B%22">Judy B</searchLink>; Department of Pediatrics, University of Texas Medical Branch, Galveston, United States.<br /><searchLink fieldCode="AU" term="%22Newman+P%22">Newman P</searchLink>; Department of Pediatrics, University of Texas Medical Branch, Galveston, United States.<br /><searchLink fieldCode="AU" term="%22Plante+JA%22">Plante JA</searchLink>; World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston, United States.; Department of Pathology, University of Texas Medical Branch, Galveston, United States.; Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, United States.<br /><searchLink fieldCode="AU" term="%22Plante+KS%22">Plante KS</searchLink>; World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston, United States.; Department of Pathology, University of Texas Medical Branch, Galveston, United States.; Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, United States.<br /><searchLink fieldCode="AU" term="%22Miller+AL%22">Miller AL</searchLink>; Department of Pediatrics, University of Texas Medical Branch, Galveston, United States.<br /><searchLink fieldCode="AU" term="%22Zhou+Y%22">Zhou Y</searchLink>; Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, United States.<br /><searchLink fieldCode="AU" term="%22Swetnam+D%22">Swetnam D</searchLink>; Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, United States.<br /><searchLink fieldCode="AU" term="%22Sotcheff+S%22">Sotcheff S</searchLink>; Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, United States.<br /><searchLink fieldCode="AU" term="%22Morris+V%22">Morris V</searchLink>; Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, United States.<br /><searchLink fieldCode="AU" term="%22Saada+N%22">Saada N</searchLink>; World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston, United States.; Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, United States.<br /><searchLink fieldCode="AU" term="%22Machado+RR%22">Machado RR</searchLink>; World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston, United States.; Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, United States.<br /><searchLink fieldCode="AU" term="%22McConnell+A%22">McConnell A</searchLink>; World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston, United States.; Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, United States.<br /><searchLink fieldCode="AU" term="%22Widen+SG%22">Widen SG</searchLink>; Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, United States.; Next-Generation Sequencing Core, The University of Texas Medical Branch, Galveston, United States.<br /><searchLink fieldCode="AU" term="%22Thompson+J%22">Thompson J</searchLink>; Next-Generation Sequencing Core, The University of Texas Medical Branch, Galveston, United States.<br /><searchLink fieldCode="AU" term="%22Dong+J%22">Dong J</searchLink>; Department of Pediatrics, University of Texas Medical Branch, Galveston, United States.; Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, United States.<br /><searchLink fieldCode="AU" term="%22Ren+P%22">Ren P</searchLink>; Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, United States.<br /><searchLink fieldCode="AU" term="%22Pyles+RB%22">Pyles RB</searchLink>; Department of Pediatrics, University of Texas Medical Branch, Galveston, United States.<br /><searchLink fieldCode="AU" term="%22Ksiazek+TG%22">Ksiazek TG</searchLink>; World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston, United States.; Department of Pathology, University of Texas Medical Branch, Galveston, United States.<br /><searchLink fieldCode="AU" term="%22Menachery+VD%22">Menachery VD</searchLink>; World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston, United States.; Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, United States.; Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, United States.<br /><searchLink fieldCode="AU" term="%22Weaver+SC%22">Weaver SC</searchLink>; World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston, United States.; Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, United States.; Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, United States.<br /><searchLink fieldCode="AU" term="%22Routh+AL%22">Routh AL</searchLink>; Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, United States.; Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, United States.; Sealy Centre for Structural Biology and Molecular Biophysics, University of Texas Medical Branch, Galveston, United States.
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