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Monitoring NLRP3 Inflammasome Activation and Exhaustion in Clinical Samples: A Refined Flow Cytometry Protocol for ASC Speck Formation Measurement Directly in Whole Blood after Ex Vivo Stimulation.

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Title: Monitoring NLRP3 Inflammasome Activation and Exhaustion in Clinical Samples: A Refined Flow Cytometry Protocol for ASC Speck Formation Measurement Directly in Whole Blood after Ex Vivo Stimulation.
Authors: Coudereau R; Immunology Laboratory, Edouard Herriot Hospital, Hospices Civils de Lyon, 5 Place d'Arsonval, CEDEX 03, 69437 Lyon, France.; EA 7426 'Pathophysiology of Injury-Induced Immunosuppression' (Université Claude Bernard Lyon 1-Hospices Civils de Lyon-bioMérieux), Joint Research Unit HCL-bioMérieux, 69003 Lyon, France., Gossez M; Immunology Laboratory, Edouard Herriot Hospital, Hospices Civils de Lyon, 5 Place d'Arsonval, CEDEX 03, 69437 Lyon, France.; CIRI, Centre International de Recherche en Infectiologie, Univ Lyon, Inserm U1111, Université Claude Bernard-Lyon 1, CNRS, UMR5308, ENS de Lyon, 69007 Lyon, France., Py BF; CIRI, Centre International de Recherche en Infectiologie, Univ Lyon, Inserm U1111, Université Claude Bernard-Lyon 1, CNRS, UMR5308, ENS de Lyon, 69007 Lyon, France., Henry T; CIRI, Centre International de Recherche en Infectiologie, Univ Lyon, Inserm U1111, Université Claude Bernard-Lyon 1, CNRS, UMR5308, ENS de Lyon, 69007 Lyon, France., Lukaszewicz AC; EA 7426 'Pathophysiology of Injury-Induced Immunosuppression' (Université Claude Bernard Lyon 1-Hospices Civils de Lyon-bioMérieux), Joint Research Unit HCL-bioMérieux, 69003 Lyon, France.; Anaesthesia and Critical Care Medicine Department, Edouard Herriot Hospital, Hospices Civils de Lyon, 5 Place d'Arsonval, CEDEX 03, 69437 Lyon, France., Monneret G; Immunology Laboratory, Edouard Herriot Hospital, Hospices Civils de Lyon, 5 Place d'Arsonval, CEDEX 03, 69437 Lyon, France.; EA 7426 'Pathophysiology of Injury-Induced Immunosuppression' (Université Claude Bernard Lyon 1-Hospices Civils de Lyon-bioMérieux), Joint Research Unit HCL-bioMérieux, 69003 Lyon, France., Venet F; Immunology Laboratory, Edouard Herriot Hospital, Hospices Civils de Lyon, 5 Place d'Arsonval, CEDEX 03, 69437 Lyon, France.; CIRI, Centre International de Recherche en Infectiologie, Univ Lyon, Inserm U1111, Université Claude Bernard-Lyon 1, CNRS, UMR5308, ENS de Lyon, 69007 Lyon, France.
Source: Cells [Cells] 2022 Oct 20; Vol. 11 (20). Date of Electronic Publication: 2022 Oct 20.
Publication Type: Journal Article; Research Support, Non-U.S. Gov't
Journal Info: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101600052 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4409 (Electronic) Linking ISSN: 20734409 NLM ISO Abbreviation: Cells Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: Monitoring NLRP3 Inflammasome Activation and Exhaustion in Clinical Samples: A Refined Flow Cytometry Protocol for ASC Speck Formation Measurement Directly in Whole Blood after Ex Vivo Stimulation.
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  Data: <searchLink fieldCode="AU" term="%22Coudereau+R%22">Coudereau R</searchLink>; Immunology Laboratory, Edouard Herriot Hospital, Hospices Civils de Lyon, 5 Place d'Arsonval, CEDEX 03, 69437 Lyon, France.; EA 7426 'Pathophysiology of Injury-Induced Immunosuppression' (Université Claude Bernard Lyon 1-Hospices Civils de Lyon-bioMérieux), Joint Research Unit HCL-bioMérieux, 69003 Lyon, France.<br /><searchLink fieldCode="AU" term="%22Gossez+M%22">Gossez M</searchLink>; Immunology Laboratory, Edouard Herriot Hospital, Hospices Civils de Lyon, 5 Place d'Arsonval, CEDEX 03, 69437 Lyon, France.; CIRI, Centre International de Recherche en Infectiologie, Univ Lyon, Inserm U1111, Université Claude Bernard-Lyon 1, CNRS, UMR5308, ENS de Lyon, 69007 Lyon, France.<br /><searchLink fieldCode="AU" term="%22Py+BF%22">Py BF</searchLink>; CIRI, Centre International de Recherche en Infectiologie, Univ Lyon, Inserm U1111, Université Claude Bernard-Lyon 1, CNRS, UMR5308, ENS de Lyon, 69007 Lyon, France.<br /><searchLink fieldCode="AU" term="%22Henry+T%22">Henry T</searchLink>; CIRI, Centre International de Recherche en Infectiologie, Univ Lyon, Inserm U1111, Université Claude Bernard-Lyon 1, CNRS, UMR5308, ENS de Lyon, 69007 Lyon, France.<br /><searchLink fieldCode="AU" term="%22Lukaszewicz+AC%22">Lukaszewicz AC</searchLink>; EA 7426 'Pathophysiology of Injury-Induced Immunosuppression' (Université Claude Bernard Lyon 1-Hospices Civils de Lyon-bioMérieux), Joint Research Unit HCL-bioMérieux, 69003 Lyon, France.; Anaesthesia and Critical Care Medicine Department, Edouard Herriot Hospital, Hospices Civils de Lyon, 5 Place d'Arsonval, CEDEX 03, 69437 Lyon, France.<br /><searchLink fieldCode="AU" term="%22Monneret+G%22">Monneret G</searchLink>; Immunology Laboratory, Edouard Herriot Hospital, Hospices Civils de Lyon, 5 Place d'Arsonval, CEDEX 03, 69437 Lyon, France.; EA 7426 'Pathophysiology of Injury-Induced Immunosuppression' (Université Claude Bernard Lyon 1-Hospices Civils de Lyon-bioMérieux), Joint Research Unit HCL-bioMérieux, 69003 Lyon, France.<br /><searchLink fieldCode="AU" term="%22Venet+F%22">Venet F</searchLink>; Immunology Laboratory, Edouard Herriot Hospital, Hospices Civils de Lyon, 5 Place d'Arsonval, CEDEX 03, 69437 Lyon, France.; CIRI, Centre International de Recherche en Infectiologie, Univ Lyon, Inserm U1111, Université Claude Bernard-Lyon 1, CNRS, UMR5308, ENS de Lyon, 69007 Lyon, France.
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              Text: 2022 Oct 20
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