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A metagenomic library cloning strategy that promotes high-level expression of captured genes to enable efficient functional screening.

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Title: A metagenomic library cloning strategy that promotes high-level expression of captured genes to enable efficient functional screening.
Authors: Rich MH; School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand., Sharrock AV; School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand; Maurice Wilkins Centre for Molecular Biodiscovery, Victoria University of Wellington, Wellington 6012, New Zealand., Mulligan TS; Department of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA., Matthews F; Department of Genetic Medicine, McKusick-Nathans Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA., Brown AS; School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand; Maurice Wilkins Centre for Molecular Biodiscovery, Victoria University of Wellington, Wellington 6012, New Zealand., Lee-Harwood HR; School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand; Maurice Wilkins Centre for Molecular Biodiscovery, Victoria University of Wellington, Wellington 6012, New Zealand., Williams EM; School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand., Copp JN; School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand., Little RF; School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand., Francis JJB; School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand., Horvat CN; School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand., Stevenson LJ; School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand; Maurice Wilkins Centre for Molecular Biodiscovery, Victoria University of Wellington, Wellington 6012, New Zealand., Owen JG; School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand; Maurice Wilkins Centre for Molecular Biodiscovery, Victoria University of Wellington, Wellington 6012, New Zealand., Saxena MT; Department of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA., Mumm JS; Department of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA; Department of Genetic Medicine, McKusick-Nathans Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA; Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA; Center for Nanomedicine, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA., Ackerley DF; School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand; Maurice Wilkins Centre for Molecular Biodiscovery, Victoria University of Wellington, Wellington 6012, New Zealand. Electronic address: david.ackerley@vuw.ac.nz.
Source: Cell chemical biology [Cell Chem Biol] 2023 Dec 21; Vol. 30 (12), pp. 1680-1691.e6. Date of Electronic Publication: 2023 Oct 27.
Publication Type: Journal Article
Journal Info: Publisher: Cell Press Country of Publication: United States NLM ID: 101676030 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2451-9448 (Electronic) Linking ISSN: 24519448 NLM ISO Abbreviation: Cell Chem Biol Subsets: MEDLINE
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  Data: A metagenomic library cloning strategy that promotes high-level expression of captured genes to enable efficient functional screening.
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  Data: <searchLink fieldCode="AU" term="%22Rich+MH%22">Rich MH</searchLink>; School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand.<br /><searchLink fieldCode="AU" term="%22Sharrock+AV%22">Sharrock AV</searchLink>; School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand; Maurice Wilkins Centre for Molecular Biodiscovery, Victoria University of Wellington, Wellington 6012, New Zealand.<br /><searchLink fieldCode="AU" term="%22Mulligan+TS%22">Mulligan TS</searchLink>; Department of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.<br /><searchLink fieldCode="AU" term="%22Matthews+F%22">Matthews F</searchLink>; Department of Genetic Medicine, McKusick-Nathans Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.<br /><searchLink fieldCode="AU" term="%22Brown+AS%22">Brown AS</searchLink>; School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand; Maurice Wilkins Centre for Molecular Biodiscovery, Victoria University of Wellington, Wellington 6012, New Zealand.<br /><searchLink fieldCode="AU" term="%22Lee-Harwood+HR%22">Lee-Harwood HR</searchLink>; School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand; Maurice Wilkins Centre for Molecular Biodiscovery, Victoria University of Wellington, Wellington 6012, New Zealand.<br /><searchLink fieldCode="AU" term="%22Williams+EM%22">Williams EM</searchLink>; School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand.<br /><searchLink fieldCode="AU" term="%22Copp+JN%22">Copp JN</searchLink>; School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand.<br /><searchLink fieldCode="AU" term="%22Little+RF%22">Little RF</searchLink>; School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand.<br /><searchLink fieldCode="AU" term="%22Francis+JJB%22">Francis JJB</searchLink>; School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand.<br /><searchLink fieldCode="AU" term="%22Horvat+CN%22">Horvat CN</searchLink>; School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand.<br /><searchLink fieldCode="AU" term="%22Stevenson+LJ%22">Stevenson LJ</searchLink>; School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand; Maurice Wilkins Centre for Molecular Biodiscovery, Victoria University of Wellington, Wellington 6012, New Zealand.<br /><searchLink fieldCode="AU" term="%22Owen+JG%22">Owen JG</searchLink>; School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand; Maurice Wilkins Centre for Molecular Biodiscovery, Victoria University of Wellington, Wellington 6012, New Zealand.<br /><searchLink fieldCode="AU" term="%22Saxena+MT%22">Saxena MT</searchLink>; Department of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.<br /><searchLink fieldCode="AU" term="%22Mumm+JS%22">Mumm JS</searchLink>; Department of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA; Department of Genetic Medicine, McKusick-Nathans Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA; Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA; Center for Nanomedicine, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.<br /><searchLink fieldCode="AU" term="%22Ackerley+DF%22">Ackerley DF</searchLink>; School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand; Maurice Wilkins Centre for Molecular Biodiscovery, Victoria University of Wellington, Wellington 6012, New Zealand. Electronic address: david.ackerley@vuw.ac.nz.
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  Data: <searchLink fieldCode="JN" term="%22101676030%22">Cell chemical biology</searchLink> [Cell Chem Biol] 2023 Dec 21; Vol. 30 (12), pp. 1680-1691.e6. <i>Date of Electronic Publication: </i>2023 Oct 27.
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