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Polycarbonate Ultracentrifuge Tube Re-use in Proteomic Analyses of Extracellular Vesicles.

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Title: Polycarbonate Ultracentrifuge Tube Re-use in Proteomic Analyses of Extracellular Vesicles.
Authors: Cahalane RME; Center for Interdisciplinary Cardiovascular Sciences, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School; Mechanobiology and Medical Device Research Group (MMDRG), Biomedical Engineering, College of Science and Engineering, University of Galway., Turner ME; Center for Interdisciplinary Cardiovascular Sciences, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School., Clift CL; Center for Interdisciplinary Cardiovascular Sciences, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School., Blaser MC; Center for Interdisciplinary Cardiovascular Sciences, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School., Bogut G; Center for Interdisciplinary Cardiovascular Sciences, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School., Levy S; Center for Interdisciplinary Cardiovascular Sciences, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School., Kasai T; Center for Interdisciplinary Cardiovascular Sciences, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School., Driedonks TAP; Department of CDL Research, University Medical Center Utrecht; Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University., Nolte-'t Hoen ENM; Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University., Aikawa M; Center for Interdisciplinary Cardiovascular Sciences, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School; Center for Excellence in Vascular Biology, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School., Singh SA; Center for Interdisciplinary Cardiovascular Sciences, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School; Center for Excellence in Vascular Biology, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School., Aikawa E; Center for Interdisciplinary Cardiovascular Sciences, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School; Center for Excellence in Vascular Biology, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School; eaikawa@bwh.harvard.edu.
Source: Journal of visualized experiments : JoVE [J Vis Exp] 2024 Mar 08 (205). Date of Electronic Publication: 2024 Mar 08.
Publication Type: Journal Article; Video-Audio Media; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural
Journal Info: Publisher: MYJoVE Corporation Country of Publication: United States NLM ID: 101313252 Publication Model: Electronic Cited Medium: Internet ISSN: 1940-087X (Electronic) Linking ISSN: 1940087X NLM ISO Abbreviation: J Vis Exp Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: <searchLink fieldCode="AU" term="%22Cahalane+RME%22">Cahalane RME</searchLink>; Center for Interdisciplinary Cardiovascular Sciences, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School; Mechanobiology and Medical Device Research Group (MMDRG), Biomedical Engineering, College of Science and Engineering, University of Galway.<br /><searchLink fieldCode="AU" term="%22Turner+ME%22">Turner ME</searchLink>; Center for Interdisciplinary Cardiovascular Sciences, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School.<br /><searchLink fieldCode="AU" term="%22Clift+CL%22">Clift CL</searchLink>; Center for Interdisciplinary Cardiovascular Sciences, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School.<br /><searchLink fieldCode="AU" term="%22Blaser+MC%22">Blaser MC</searchLink>; Center for Interdisciplinary Cardiovascular Sciences, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School.<br /><searchLink fieldCode="AU" term="%22Bogut+G%22">Bogut G</searchLink>; Center for Interdisciplinary Cardiovascular Sciences, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School.<br /><searchLink fieldCode="AU" term="%22Levy+S%22">Levy S</searchLink>; Center for Interdisciplinary Cardiovascular Sciences, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School.<br /><searchLink fieldCode="AU" term="%22Kasai+T%22">Kasai T</searchLink>; Center for Interdisciplinary Cardiovascular Sciences, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School.<br /><searchLink fieldCode="AU" term="%22Driedonks+TAP%22">Driedonks TAP</searchLink>; Department of CDL Research, University Medical Center Utrecht; Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University.<br /><searchLink fieldCode="AU" term="%22Nolte-'t+Hoen+ENM%22">Nolte-'t Hoen ENM</searchLink>; Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University.<br /><searchLink fieldCode="AU" term="%22Aikawa+M%22">Aikawa M</searchLink>; Center for Interdisciplinary Cardiovascular Sciences, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School; Center for Excellence in Vascular Biology, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School.<br /><searchLink fieldCode="AU" term="%22Singh+SA%22">Singh SA</searchLink>; Center for Interdisciplinary Cardiovascular Sciences, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School; Center for Excellence in Vascular Biology, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School.<br /><searchLink fieldCode="AU" term="%22Aikawa+E%22">Aikawa E</searchLink>; Center for Interdisciplinary Cardiovascular Sciences, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School; Center for Excellence in Vascular Biology, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School; eaikawa@bwh.harvard.edu.
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            – D: 08
              M: 03
              Text: 2024 Mar 08
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