Guidelines for preparation and flow cytometry analysis of human nonlymphoid tissue DC.

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Title: Guidelines for preparation and flow cytometry analysis of human nonlymphoid tissue DC.
Authors: Dudziak D; Institute of Immunology, Jena University Hospital, Friedrich-Schiller-University, Jena, Germany.; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Erlangen, Germany., Heger L; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Erlangen, Germany.; Department of Transfusion Medicine and Hemostaseology, University Hospital Erlangen, Erlangen, Germany., Agace WW; LEO Foundation Skin Immunology Research Center, Department of Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark.; Immunology Section, Lund University, Lund, Sweden., Bakker J; Institute for Infection and Immunology, Cancer Immunology, Amsterdam, The Netherlands.; Cancer Center Amsterdam, Cancer Immunology, Amsterdam, The Netherlands.; Amsterdam UMC location Vrije Universiteit, Medical Oncology, Amsterdam, The Netherlands., de Gruijl TD; Institute for Infection and Immunology, Cancer Immunology, Amsterdam, The Netherlands.; Cancer Center Amsterdam, Cancer Immunology, Amsterdam, The Netherlands.; Amsterdam UMC location Vrije Universiteit, Medical Oncology, Amsterdam, The Netherlands., Dress RJ; Institute of Systems Immunology, Hamburg Center for Translational Immunology (HCTI), University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Dutertre CA; Inserm U1015, Gustave Roussy, Villejuif, France., Fenton TM; School of Infection and Immunity, University of Glasgow, Glasgow, UK., Fransen MF; Institute for Infection and Immunology, Cancer Immunology, Amsterdam, The Netherlands.; Cancer Center Amsterdam, Cancer Immunology, Amsterdam, The Netherlands.; Department of Pulmonary Diseases, Amsterdam UMC location Vrije Universiteit, Amsterdam, The Netherlands., Ginhoux F; Singapore Immunology Network (SIgN), Agency for Science, Technology and Research, Singapore, Singapore.; Department of Immunology and Microbiology, Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.; SingHealth Duke-NUS Academic Medical Centre, Translational Immunology Institute, Singapore, Singapore.; INSERM U1015, Gustave Roussy Cancer Campus, Villejuif, France., Heyman O; Department of Periodontology, Hadassah Medical Center, Faculty of Dental Medicine, Hebrew University of Jerusalem, Israel., Horev Y; Department of Periodontology, Hadassah Medical Center, Faculty of Dental Medicine, Hebrew University of Jerusalem, Israel., Hornsteiner F; Department of Dermatology, Venereology & Allergology, Medical University of Innsbruck, Innsbruck, Austria., Kandiah V; Institute for Infection and Immunology, Cancer Immunology, Amsterdam, The Netherlands.; Cancer Center Amsterdam, Cancer Immunology, Amsterdam, The Netherlands.; Amsterdam UMC location Vrije Universiteit, Medical Oncology, Amsterdam, The Netherlands., Kles P; Department of Periodontology, Hadassah Medical Center, Faculty of Dental Medicine, Hebrew University of Jerusalem, Israel., Lubin R; Faculty of Dental Medicine, The Institute of Biomedical and Oral Research, Hebrew University of Jerusalem, Israel., Mizraji G; Department of Periodontology, Hadassah Medical Center, Faculty of Dental Medicine, Hebrew University of Jerusalem, Israel., Prokopi A; Institute for Infection and Immunology, Cancer Immunology, Amsterdam, The Netherlands.; Cancer Center Amsterdam, Cancer Immunology, Amsterdam, The Netherlands.; Amsterdam UMC location Vrije Universiteit, Medical Oncology, Amsterdam, The Netherlands., Saar O; Department of Periodontology, Hadassah Medical Center, Faculty of Dental Medicine, Hebrew University of Jerusalem, Israel., Sopper S; Internal Medicine V, Hematology and Oncology, Medical University of Innsbruck, Innsbruck, Austria.; Tyrolean Cancer Research Center, Innsbruck, Austria., Stoitzner P; Department of Dermatology, Venereology & Allergology, Medical University of Innsbruck, Innsbruck, Austria., Strandt H; Department of Dermatology, Venereology & Allergology, Medical University of Innsbruck, Innsbruck, Austria., Sykora MM; Internal Medicine V, Hematology and Oncology, Medical University of Innsbruck, Innsbruck, Austria.; Tyrolean Cancer Research Center, Innsbruck, Austria., Toffoli EC; Institute for Infection and Immunology, Cancer Immunology, Amsterdam, The Netherlands.; Cancer Center Amsterdam, Cancer Immunology, Amsterdam, The Netherlands.; Amsterdam UMC location Vrije Universiteit, Medical Oncology, Amsterdam, The Netherlands., Tripp CH; Department of Dermatology, Venereology & Allergology, Medical University of Innsbruck, Innsbruck, Austria., van Pul K; Institute for Infection and Immunology, Cancer Immunology, Amsterdam, The Netherlands.; Cancer Center Amsterdam, Cancer Immunology, Amsterdam, The Netherlands.; Amsterdam UMC location Vrije Universiteit, Medical Oncology, Amsterdam, The Netherlands., van de Ven R; Cancer Center Amsterdam, Cancer Immunology, Amsterdam, The Netherlands.; Amsterdam UMC location Vrije Universiteit, Medical Oncology, Amsterdam, The Netherlands.; Department of Otolaryngology, Head and Neck Surgery, Amsterdam UMC location Vrije Universiteit, Amsterdam, The Netherlands., Wilensky A; Department of Periodontology, Hadassah Medical Center, Faculty of Dental Medicine, Hebrew University of Jerusalem, Israel., Yona S; Faculty of Dental Medicine, The Institute of Biomedical and Oral Research, Hebrew University of Jerusalem, Israel., Zelle-Rieser C; Department of Dermatology, Venereology & Allergology, Medical University of Innsbruck, Innsbruck, Austria.
Source: European journal of immunology [Eur J Immunol] 2025 Jan; Vol. 55 (1), pp. e2250325. Date of Electronic Publication: 2024 Dec 12.
Publication Type: Journal Article
Journal Info: Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 1273201 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1521-4141 (Electronic) Linking ISSN: 00142980 NLM ISO Abbreviation: Eur J Immunol Subsets: MEDLINE
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  Data: Guidelines for preparation and flow cytometry analysis of human nonlymphoid tissue DC.
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  Data: <searchLink fieldCode="AU" term="%22Dudziak+D%22">Dudziak D</searchLink>; Institute of Immunology, Jena University Hospital, Friedrich-Schiller-University, Jena, Germany.; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Erlangen, Germany.<br /><searchLink fieldCode="AU" term="%22Heger+L%22">Heger L</searchLink>; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Erlangen, Germany.; Department of Transfusion Medicine and Hemostaseology, University Hospital Erlangen, Erlangen, Germany.<br /><searchLink fieldCode="AU" term="%22Agace+WW%22">Agace WW</searchLink>; LEO Foundation Skin Immunology Research Center, Department of Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark.; Immunology Section, Lund University, Lund, Sweden.<br /><searchLink fieldCode="AU" term="%22Bakker+J%22">Bakker J</searchLink>; Institute for Infection and Immunology, Cancer Immunology, Amsterdam, The Netherlands.; Cancer Center Amsterdam, Cancer Immunology, Amsterdam, The Netherlands.; Amsterdam UMC location Vrije Universiteit, Medical Oncology, Amsterdam, The Netherlands.<br /><searchLink fieldCode="AU" term="%22de+Gruijl+TD%22">de Gruijl TD</searchLink>; Institute for Infection and Immunology, Cancer Immunology, Amsterdam, The Netherlands.; Cancer Center Amsterdam, Cancer Immunology, Amsterdam, The Netherlands.; Amsterdam UMC location Vrije Universiteit, Medical Oncology, Amsterdam, The Netherlands.<br /><searchLink fieldCode="AU" term="%22Dress+RJ%22">Dress RJ</searchLink>; Institute of Systems Immunology, Hamburg Center for Translational Immunology (HCTI), University Medical Center Hamburg-Eppendorf, Hamburg, Germany.<br /><searchLink fieldCode="AU" term="%22Dutertre+CA%22">Dutertre CA</searchLink>; Inserm U1015, Gustave Roussy, Villejuif, France.<br /><searchLink fieldCode="AU" term="%22Fenton+TM%22">Fenton TM</searchLink>; School of Infection and Immunity, University of Glasgow, Glasgow, UK.<br /><searchLink fieldCode="AU" term="%22Fransen+MF%22">Fransen MF</searchLink>; Institute for Infection and Immunology, Cancer Immunology, Amsterdam, The Netherlands.; Cancer Center Amsterdam, Cancer Immunology, Amsterdam, The Netherlands.; Department of Pulmonary Diseases, Amsterdam UMC location Vrije Universiteit, Amsterdam, The Netherlands.<br /><searchLink fieldCode="AU" term="%22Ginhoux+F%22">Ginhoux F</searchLink>; Singapore Immunology Network (SIgN), Agency for Science, Technology and Research, Singapore, Singapore.; Department of Immunology and Microbiology, Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.; SingHealth Duke-NUS Academic Medical Centre, Translational Immunology Institute, Singapore, Singapore.; INSERM U1015, Gustave Roussy Cancer Campus, Villejuif, France.<br /><searchLink fieldCode="AU" term="%22Heyman+O%22">Heyman O</searchLink>; Department of Periodontology, Hadassah Medical Center, Faculty of Dental Medicine, Hebrew University of Jerusalem, Israel.<br /><searchLink fieldCode="AU" term="%22Horev+Y%22">Horev Y</searchLink>; Department of Periodontology, Hadassah Medical Center, Faculty of Dental Medicine, Hebrew University of Jerusalem, Israel.<br /><searchLink fieldCode="AU" term="%22Hornsteiner+F%22">Hornsteiner F</searchLink>; Department of Dermatology, Venereology & Allergology, Medical University of Innsbruck, Innsbruck, Austria.<br /><searchLink fieldCode="AU" term="%22Kandiah+V%22">Kandiah V</searchLink>; Institute for Infection and Immunology, Cancer Immunology, Amsterdam, The Netherlands.; Cancer Center Amsterdam, Cancer Immunology, Amsterdam, The Netherlands.; Amsterdam UMC location Vrije Universiteit, Medical Oncology, Amsterdam, The Netherlands.<br /><searchLink fieldCode="AU" term="%22Kles+P%22">Kles P</searchLink>; Department of Periodontology, Hadassah Medical Center, Faculty of Dental Medicine, Hebrew University of Jerusalem, Israel.<br /><searchLink fieldCode="AU" term="%22Lubin+R%22">Lubin R</searchLink>; Faculty of Dental Medicine, The Institute of Biomedical and Oral Research, Hebrew University of Jerusalem, Israel.<br /><searchLink fieldCode="AU" term="%22Mizraji+G%22">Mizraji G</searchLink>; Department of Periodontology, Hadassah Medical Center, Faculty of Dental Medicine, Hebrew University of Jerusalem, Israel.<br /><searchLink fieldCode="AU" term="%22Prokopi+A%22">Prokopi A</searchLink>; Institute for Infection and Immunology, Cancer Immunology, Amsterdam, The Netherlands.; Cancer Center Amsterdam, Cancer Immunology, Amsterdam, The Netherlands.; Amsterdam UMC location Vrije Universiteit, Medical Oncology, Amsterdam, The Netherlands.<br /><searchLink fieldCode="AU" term="%22Saar+O%22">Saar O</searchLink>; Department of Periodontology, Hadassah Medical Center, Faculty of Dental Medicine, Hebrew University of Jerusalem, Israel.<br /><searchLink fieldCode="AU" term="%22Sopper+S%22">Sopper S</searchLink>; Internal Medicine V, Hematology and Oncology, Medical University of Innsbruck, Innsbruck, Austria.; Tyrolean Cancer Research Center, Innsbruck, Austria.<br /><searchLink fieldCode="AU" term="%22Stoitzner+P%22">Stoitzner P</searchLink>; Department of Dermatology, Venereology & Allergology, Medical University of Innsbruck, Innsbruck, Austria.<br /><searchLink fieldCode="AU" term="%22Strandt+H%22">Strandt H</searchLink>; Department of Dermatology, Venereology & Allergology, Medical University of Innsbruck, Innsbruck, Austria.<br /><searchLink fieldCode="AU" term="%22Sykora+MM%22">Sykora MM</searchLink>; Internal Medicine V, Hematology and Oncology, Medical University of Innsbruck, Innsbruck, Austria.; Tyrolean Cancer Research Center, Innsbruck, Austria.<br /><searchLink fieldCode="AU" term="%22Toffoli+EC%22">Toffoli EC</searchLink>; Institute for Infection and Immunology, Cancer Immunology, Amsterdam, The Netherlands.; Cancer Center Amsterdam, Cancer Immunology, Amsterdam, The Netherlands.; Amsterdam UMC location Vrije Universiteit, Medical Oncology, Amsterdam, The Netherlands.<br /><searchLink fieldCode="AU" term="%22Tripp+CH%22">Tripp CH</searchLink>; Department of Dermatology, Venereology & Allergology, Medical University of Innsbruck, Innsbruck, Austria.<br /><searchLink fieldCode="AU" term="%22van+Pul+K%22">van Pul K</searchLink>; Institute for Infection and Immunology, Cancer Immunology, Amsterdam, The Netherlands.; Cancer Center Amsterdam, Cancer Immunology, Amsterdam, The Netherlands.; Amsterdam UMC location Vrije Universiteit, Medical Oncology, Amsterdam, The Netherlands.<br /><searchLink fieldCode="AU" term="%22van+de+Ven+R%22">van de Ven R</searchLink>; Cancer Center Amsterdam, Cancer Immunology, Amsterdam, The Netherlands.; Amsterdam UMC location Vrije Universiteit, Medical Oncology, Amsterdam, The Netherlands.; Department of Otolaryngology, Head and Neck Surgery, Amsterdam UMC location Vrije Universiteit, Amsterdam, The Netherlands.<br /><searchLink fieldCode="AU" term="%22Wilensky+A%22">Wilensky A</searchLink>; Department of Periodontology, Hadassah Medical Center, Faculty of Dental Medicine, Hebrew University of Jerusalem, Israel.<br /><searchLink fieldCode="AU" term="%22Yona+S%22">Yona S</searchLink>; Faculty of Dental Medicine, The Institute of Biomedical and Oral Research, Hebrew University of Jerusalem, Israel.<br /><searchLink fieldCode="AU" term="%22Zelle-Rieser+C%22">Zelle-Rieser C</searchLink>; Department of Dermatology, Venereology & Allergology, Medical University of Innsbruck, Innsbruck, Austria.
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