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Optimized isolation of enzymatically active ubiquitin E3 ligase E6AP/UBE3A from mammalian cells.

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Title: Optimized isolation of enzymatically active ubiquitin E3 ligase E6AP/UBE3A from mammalian cells.
Authors: Schafer JM; Protein Processing Section, Center for Structural Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, 21702, USA., Muli CS; Protein Processing Section, Center for Structural Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, 21702, USA., Heikal RA; Protein Processing Section, Center for Structural Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, 21702, USA; Department of Chemistry, University of Massachusetts, Amherst, MA, 01003, USA., Dyba MA; Biophysics Resource, Center for Structural Biology, National Cancer Institute, National Institutes of Health, Frederick, MD, 21702, USA., Tarasov SG; Biophysics Resource, Center for Structural Biology, National Cancer Institute, National Institutes of Health, Frederick, MD, 21702, USA., Stratton MM; Molecular and Cellular Biology Graduate Program, College of Natural Sciences, University of Massachusetts, Amherst, MA, 01003, USA; Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA, 01003, USA., Strieter ER; Department of Chemistry, University of Massachusetts, Amherst, MA, 01003, USA; Molecular and Cellular Biology Graduate Program, College of Natural Sciences, University of Massachusetts, Amherst, MA, 01003, USA., Walters KJ; Protein Processing Section, Center for Structural Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, 21702, USA. Electronic address: kylie.walters@nih.gov.
Source: Protein expression and purification [Protein Expr Purif] 2025 Apr; Vol. 228, pp. 106661. Date of Electronic Publication: 2025 Jan 09.
Publication Type: Journal Article
Journal Info: Publisher: Academic Press Country of Publication: United States NLM ID: 9101496 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0279 (Electronic) Linking ISSN: 10465928 NLM ISO Abbreviation: Protein Expr Purif Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: Optimized isolation of enzymatically active ubiquitin E3 ligase E6AP/UBE3A from mammalian cells.
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  Data: <searchLink fieldCode="AU" term="%22Schafer+JM%22">Schafer JM</searchLink>; Protein Processing Section, Center for Structural Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, 21702, USA.<br /><searchLink fieldCode="AU" term="%22Muli+CS%22">Muli CS</searchLink>; Protein Processing Section, Center for Structural Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, 21702, USA.<br /><searchLink fieldCode="AU" term="%22Heikal+RA%22">Heikal RA</searchLink>; Protein Processing Section, Center for Structural Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, 21702, USA; Department of Chemistry, University of Massachusetts, Amherst, MA, 01003, USA.<br /><searchLink fieldCode="AU" term="%22Dyba+MA%22">Dyba MA</searchLink>; Biophysics Resource, Center for Structural Biology, National Cancer Institute, National Institutes of Health, Frederick, MD, 21702, USA.<br /><searchLink fieldCode="AU" term="%22Tarasov+SG%22">Tarasov SG</searchLink>; Biophysics Resource, Center for Structural Biology, National Cancer Institute, National Institutes of Health, Frederick, MD, 21702, USA.<br /><searchLink fieldCode="AU" term="%22Stratton+MM%22">Stratton MM</searchLink>; Molecular and Cellular Biology Graduate Program, College of Natural Sciences, University of Massachusetts, Amherst, MA, 01003, USA; Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA, 01003, USA.<br /><searchLink fieldCode="AU" term="%22Strieter+ER%22">Strieter ER</searchLink>; Department of Chemistry, University of Massachusetts, Amherst, MA, 01003, USA; Molecular and Cellular Biology Graduate Program, College of Natural Sciences, University of Massachusetts, Amherst, MA, 01003, USA.<br /><searchLink fieldCode="AU" term="%22Walters+KJ%22">Walters KJ</searchLink>; Protein Processing Section, Center for Structural Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, 21702, USA. Electronic address: kylie.walters@nih.gov.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Academic+Press%22">Academic Press </searchLink><i>Country of Publication: </i>United States <i>NLM ID: </i>9101496 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1096-0279 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2210465928%22">10465928 </searchLink><i>NLM ISO Abbreviation: </i>Protein Expr Purif <i>Subsets: </i>MEDLINE
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        Value: 10.1016/j.pep.2025.106661
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        Text: English
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              Text: 2025 Apr
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