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Comparative analysis of empty and full adeno-associated viruses under stress conditions by anion-exchange chromatography, analytical ultracentrifugation, and mass photometry.

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Title: Comparative analysis of empty and full adeno-associated viruses under stress conditions by anion-exchange chromatography, analytical ultracentrifugation, and mass photometry.
Authors: Townsend JA; Analytical Chemistry, Regeneron Pharmaceuticals Inc., Tarrytown, NY, USA., Li S; Formulation Development, Regeneron Pharmaceuticals Inc., Tarrytown, NY, USA., Sweezy L; Protein Biochemistry, Regeneron Pharmaceuticals Inc., Tarrytown, NY, USA., Liu N; Protein Biochemistry, Regeneron Pharmaceuticals Inc., Tarrytown, NY, USA., Rosconi MP; Protein Biochemistry, Regeneron Pharmaceuticals Inc., Tarrytown, NY, USA., Pyles EA; Protein Biochemistry, Regeneron Pharmaceuticals Inc., Tarrytown, NY, USA., Zhi L; Formulation Development, Regeneron Pharmaceuticals Inc., Tarrytown, NY, USA., Liu D; Formulation Development, Regeneron Pharmaceuticals Inc., Tarrytown, NY, USA., Wu Z; Analytical Chemistry, Regeneron Pharmaceuticals Inc., Tarrytown, NY, USA. Electronic address: zhijie.wu@regeneron.com., Qiu H; Analytical Chemistry, Regeneron Pharmaceuticals Inc., Tarrytown, NY, USA. Electronic address: haibo.qiu@regeneron.com., Shameem M; Formulation Development, Regeneron Pharmaceuticals Inc., Tarrytown, NY, USA., Li N; Analytical Chemistry, Regeneron Pharmaceuticals Inc., Tarrytown, NY, USA.
Source: Journal of pharmaceutical sciences [J Pharm Sci] 2025 Feb; Vol. 114 (2), pp. 1237-1244. Date of Electronic Publication: 2025 Jan 17.
Publication Type: Journal Article; Comparative Study
Journal Info: Publisher: Elsevier Country of Publication: United States NLM ID: 2985195R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6017 (Electronic) Linking ISSN: 00223549 NLM ISO Abbreviation: J Pharm Sci Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: Comparative analysis of empty and full adeno-associated viruses under stress conditions by anion-exchange chromatography, analytical ultracentrifugation, and mass photometry.
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  Data: <searchLink fieldCode="AU" term="%22Townsend+JA%22">Townsend JA</searchLink>; Analytical Chemistry, Regeneron Pharmaceuticals Inc., Tarrytown, NY, USA.<br /><searchLink fieldCode="AU" term="%22Li+S%22">Li S</searchLink>; Formulation Development, Regeneron Pharmaceuticals Inc., Tarrytown, NY, USA.<br /><searchLink fieldCode="AU" term="%22Sweezy+L%22">Sweezy L</searchLink>; Protein Biochemistry, Regeneron Pharmaceuticals Inc., Tarrytown, NY, USA.<br /><searchLink fieldCode="AU" term="%22Liu+N%22">Liu N</searchLink>; Protein Biochemistry, Regeneron Pharmaceuticals Inc., Tarrytown, NY, USA.<br /><searchLink fieldCode="AU" term="%22Rosconi+MP%22">Rosconi MP</searchLink>; Protein Biochemistry, Regeneron Pharmaceuticals Inc., Tarrytown, NY, USA.<br /><searchLink fieldCode="AU" term="%22Pyles+EA%22">Pyles EA</searchLink>; Protein Biochemistry, Regeneron Pharmaceuticals Inc., Tarrytown, NY, USA.<br /><searchLink fieldCode="AU" term="%22Zhi+L%22">Zhi L</searchLink>; Formulation Development, Regeneron Pharmaceuticals Inc., Tarrytown, NY, USA.<br /><searchLink fieldCode="AU" term="%22Liu+D%22">Liu D</searchLink>; Formulation Development, Regeneron Pharmaceuticals Inc., Tarrytown, NY, USA.<br /><searchLink fieldCode="AU" term="%22Wu+Z%22">Wu Z</searchLink>; Analytical Chemistry, Regeneron Pharmaceuticals Inc., Tarrytown, NY, USA. Electronic address: zhijie.wu@regeneron.com.<br /><searchLink fieldCode="AU" term="%22Qiu+H%22">Qiu H</searchLink>; Analytical Chemistry, Regeneron Pharmaceuticals Inc., Tarrytown, NY, USA. Electronic address: haibo.qiu@regeneron.com.<br /><searchLink fieldCode="AU" term="%22Shameem+M%22">Shameem M</searchLink>; Formulation Development, Regeneron Pharmaceuticals Inc., Tarrytown, NY, USA.<br /><searchLink fieldCode="AU" term="%22Li+N%22">Li N</searchLink>; Analytical Chemistry, Regeneron Pharmaceuticals Inc., Tarrytown, NY, USA.
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  Data: <searchLink fieldCode="JN" term="%222985195R%22">Journal of pharmaceutical sciences</searchLink> [J Pharm Sci] 2025 Feb; Vol. 114 (2), pp. 1237-1244. <i>Date of Electronic Publication: </i>2025 Jan 17.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Elsevier%22">Elsevier </searchLink><i>Country of Publication: </i>United States <i>NLM ID: </i>2985195R <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1520-6017 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2200223549%22">00223549 </searchLink><i>NLM ISO Abbreviation: </i>J Pharm Sci <i>Subsets: </i>MEDLINE
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