The Mycobacterium ulcerans toxin mycolactone causes destructive Sec61-dependent loss of the endothelial glycocalyx and vessel basement membrane to drive skin necrosis. - Hsieh LT; Hall BS; Newcombe J; Mendum TA; Varela SS; Umrania Y; Deery MJ; Shi WQ; Diaz-Delgado J; Salguero FJ; Simmonds RE (ELife, 2025)