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A target affinity enrichment workflow to characterize critical post-translational modifications within therapeutic antibodies.

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Title: A target affinity enrichment workflow to characterize critical post-translational modifications within therapeutic antibodies.
Authors: Prinston JE; Protein Biochemistry, Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, NY 10591, United States., Peng W; Analytical Chemistry, Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, NY 10591, United States., Provoncha K; Therapeutic Proteins, Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, NY 10591, United States., Moon Y; Protein Biochemistry, Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, NY 10591, United States., Koufos E; Protein Biochemistry, Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, NY 10591, United States., Sandu C; Therapeutic Proteins, Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, NY 10591, United States., Fu Y; Protein Biochemistry, Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, NY 10591, United States., Yan Y; Analytical Chemistry, Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, NY 10591, United States., Wang S; Analytical Chemistry, Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, NY 10591, United States., Li N; Analytical Chemistry, Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, NY 10591, United States., Nguyen JB; Protein Biochemistry, Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, NY 10591, United States. Electronic address: jennifer.nguyen@regeneron.com., Rosconi MP; Protein Biochemistry, Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, NY 10591, United States., Pyles EA; Protein Biochemistry, Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, NY 10591, United States.
Source: Journal of pharmaceutical sciences [J Pharm Sci] 2025 May; Vol. 114 (5), pp. 103710. Date of Electronic Publication: 2025 Feb 27.
Publication Type: Journal Article
Journal Info: Publisher: Elsevier Country of Publication: United States NLM ID: 2985195R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6017 (Electronic) Linking ISSN: 00223549 NLM ISO Abbreviation: J Pharm Sci Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: <searchLink fieldCode="AU" term="%22Prinston+JE%22">Prinston JE</searchLink>; Protein Biochemistry, Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, NY 10591, United States.<br /><searchLink fieldCode="AU" term="%22Peng+W%22">Peng W</searchLink>; Analytical Chemistry, Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, NY 10591, United States.<br /><searchLink fieldCode="AU" term="%22Provoncha+K%22">Provoncha K</searchLink>; Therapeutic Proteins, Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, NY 10591, United States.<br /><searchLink fieldCode="AU" term="%22Moon+Y%22">Moon Y</searchLink>; Protein Biochemistry, Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, NY 10591, United States.<br /><searchLink fieldCode="AU" term="%22Koufos+E%22">Koufos E</searchLink>; Protein Biochemistry, Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, NY 10591, United States.<br /><searchLink fieldCode="AU" term="%22Sandu+C%22">Sandu C</searchLink>; Therapeutic Proteins, Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, NY 10591, United States.<br /><searchLink fieldCode="AU" term="%22Fu+Y%22">Fu Y</searchLink>; Protein Biochemistry, Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, NY 10591, United States.<br /><searchLink fieldCode="AU" term="%22Yan+Y%22">Yan Y</searchLink>; Analytical Chemistry, Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, NY 10591, United States.<br /><searchLink fieldCode="AU" term="%22Wang+S%22">Wang S</searchLink>; Analytical Chemistry, Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, NY 10591, United States.<br /><searchLink fieldCode="AU" term="%22Li+N%22">Li N</searchLink>; Analytical Chemistry, Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, NY 10591, United States.<br /><searchLink fieldCode="AU" term="%22Nguyen+JB%22">Nguyen JB</searchLink>; Protein Biochemistry, Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, NY 10591, United States. Electronic address: jennifer.nguyen@regeneron.com.<br /><searchLink fieldCode="AU" term="%22Rosconi+MP%22">Rosconi MP</searchLink>; Protein Biochemistry, Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, NY 10591, United States.<br /><searchLink fieldCode="AU" term="%22Pyles+EA%22">Pyles EA</searchLink>; Protein Biochemistry, Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, NY 10591, United States.
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