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Peptide-enabled ribonucleoprotein delivery for CRISPR engineering (PERC) in primary human immune cells and hematopoietic stem cells.

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Title: Peptide-enabled ribonucleoprotein delivery for CRISPR engineering (PERC) in primary human immune cells and hematopoietic stem cells.
Authors: Sahu SU; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA.; Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA, USA.; California Institute for Quantitative Biosciences at University of California Berkeley, Berkeley, CA, USA., Castro M; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA.; Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA, USA.; California Institute for Quantitative Biosciences at University of California Berkeley, Berkeley, CA, USA., Muldoon JJ; Gladstone-UCSF Institute of Genomic Immunology, San Francisco, CA, USA.; Department of Medicine, University of California San Francisco, San Francisco, CA, USA., Asija K; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA.; Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA, USA.; California Institute for Quantitative Biosciences at University of California Berkeley, Berkeley, CA, USA., Wyman SK; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA., Krishnappa N; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA., de Oñate L; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA.; Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA, USA., Eyquem J; Gladstone-UCSF Institute of Genomic Immunology, San Francisco, CA, USA.; Department of Medicine, University of California San Francisco, San Francisco, CA, USA.; Parker Institute for Cancer Immunotherapy, University of California San Francisco, San Francisco, CA, USA.; Department of Microbiology and Immunology, University of California San Francisco, San Francisco, CA, USA.; UCSF Helen Diller Family Comprehensive Cancer Center, University of California San Francisco, San Francisco, CA, USA.; Institute for Human Genetics, University of California San Francisco, San Francisco, CA, USA., Nguyen DN; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA.; Gladstone-UCSF Institute of Genomic Immunology, San Francisco, CA, USA.; Department of Medicine, University of California San Francisco, San Francisco, CA, USA., Wilson RC; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA. rosswilson@berkeley.edu.; Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA, USA. rosswilson@berkeley.edu.; California Institute for Quantitative Biosciences at University of California Berkeley, Berkeley, CA, USA. rosswilson@berkeley.edu.
Source: Nature protocols [Nat Protoc] 2025 Oct; Vol. 20 (10), pp. 2735-2770. Date of Electronic Publication: 2025 Mar 03.
Publication Type: Journal Article; Review
Journal Info: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101284307 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1750-2799 (Electronic) Linking ISSN: 17502799 NLM ISO Abbreviation: Nat Protoc Subsets: MEDLINE
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  Data: Peptide-enabled ribonucleoprotein delivery for CRISPR engineering (PERC) in primary human immune cells and hematopoietic stem cells.
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  Data: <searchLink fieldCode="AU" term="%22Sahu+SU%22">Sahu SU</searchLink>; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA.; Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA, USA.; California Institute for Quantitative Biosciences at University of California Berkeley, Berkeley, CA, USA.<br /><searchLink fieldCode="AU" term="%22Castro+M%22">Castro M</searchLink>; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA.; Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA, USA.; California Institute for Quantitative Biosciences at University of California Berkeley, Berkeley, CA, USA.<br /><searchLink fieldCode="AU" term="%22Muldoon+JJ%22">Muldoon JJ</searchLink>; Gladstone-UCSF Institute of Genomic Immunology, San Francisco, CA, USA.; Department of Medicine, University of California San Francisco, San Francisco, CA, USA.<br /><searchLink fieldCode="AU" term="%22Asija+K%22">Asija K</searchLink>; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA.; Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA, USA.; California Institute for Quantitative Biosciences at University of California Berkeley, Berkeley, CA, USA.<br /><searchLink fieldCode="AU" term="%22Wyman+SK%22">Wyman SK</searchLink>; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA.<br /><searchLink fieldCode="AU" term="%22Krishnappa+N%22">Krishnappa N</searchLink>; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA.<br /><searchLink fieldCode="AU" term="%22de+Oñate+L%22">de Oñate L</searchLink>; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA.; Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA, USA.<br /><searchLink fieldCode="AU" term="%22Eyquem+J%22">Eyquem J</searchLink>; Gladstone-UCSF Institute of Genomic Immunology, San Francisco, CA, USA.; Department of Medicine, University of California San Francisco, San Francisco, CA, USA.; Parker Institute for Cancer Immunotherapy, University of California San Francisco, San Francisco, CA, USA.; Department of Microbiology and Immunology, University of California San Francisco, San Francisco, CA, USA.; UCSF Helen Diller Family Comprehensive Cancer Center, University of California San Francisco, San Francisco, CA, USA.; Institute for Human Genetics, University of California San Francisco, San Francisco, CA, USA.<br /><searchLink fieldCode="AU" term="%22Nguyen+DN%22">Nguyen DN</searchLink>; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA.; Gladstone-UCSF Institute of Genomic Immunology, San Francisco, CA, USA.; Department of Medicine, University of California San Francisco, San Francisco, CA, USA.<br /><searchLink fieldCode="AU" term="%22Wilson+RC%22">Wilson RC</searchLink>; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA. rosswilson@berkeley.edu.; Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA, USA. rosswilson@berkeley.edu.; California Institute for Quantitative Biosciences at University of California Berkeley, Berkeley, CA, USA. rosswilson@berkeley.edu.
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  Data: <searchLink fieldCode="JN" term="%22101284307%22">Nature protocols</searchLink> [Nat Protoc] 2025 Oct; Vol. 20 (10), pp. 2735-2770. <i>Date of Electronic Publication: </i>2025 Mar 03.
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              Text: 2025 Oct
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