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Protocol to purify and culture human pancreatic cancer cells from patient-derived xenografts.

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Title: Protocol to purify and culture human pancreatic cancer cells from patient-derived xenografts.
Authors: Tessier J; Cancer Early Detection Advanced Research Center at Knight Cancer Institute, Oregon Health & Science University (OHSU) School of Medicine, Portland, OR 97201, USA., Grygoryev D; Cancer Early Detection Advanced Research Center at Knight Cancer Institute, Oregon Health & Science University (OHSU) School of Medicine, Portland, OR 97201, USA; Department of Molecular and Medical Genetics, OHSU School of Medicine, Portland, OR 97201, USA., Chow-Castro M; Cancer Early Detection Advanced Research Center at Knight Cancer Institute, Oregon Health & Science University (OHSU) School of Medicine, Portland, OR 97201, USA., Ekstrom T; Cancer Early Detection Advanced Research Center at Knight Cancer Institute, Oregon Health & Science University (OHSU) School of Medicine, Portland, OR 97201, USA., Manalo E; Cancer Early Detection Advanced Research Center at Knight Cancer Institute, Oregon Health & Science University (OHSU) School of Medicine, Portland, OR 97201, USA., Lee SW; Cancer Early Detection Advanced Research Center at Knight Cancer Institute, Oregon Health & Science University (OHSU) School of Medicine, Portland, OR 97201, USA; Department of Molecular and Medical Genetics, OHSU School of Medicine, Portland, OR 97201, USA., Berry M; Cancer Early Detection Advanced Research Center at Knight Cancer Institute, Oregon Health & Science University (OHSU) School of Medicine, Portland, OR 97201, USA., Link JM; Department of Molecular and Medical Genetics, OHSU School of Medicine, Portland, OR 97201, USA; Brenden-Colson Center for Pancreatic Care, OHSU School of Medicine, Portland, OR 97201, USA., Keith D; Brenden-Colson Center for Pancreatic Care, OHSU School of Medicine, Portland, OR 97201, USA., Allen-Petersen BL; Department of Molecular and Medical Genetics, OHSU School of Medicine, Portland, OR 97201, USA; Brenden-Colson Center for Pancreatic Care, OHSU School of Medicine, Portland, OR 97201, USA., Sheppard B; Brenden-Colson Center for Pancreatic Care, OHSU School of Medicine, Portland, OR 97201, USA; Department of Surgery, OHSU School of Medicine, Portland, OR 97201, USA., Morgan T; Cancer Early Detection Advanced Research Center at Knight Cancer Institute, Oregon Health & Science University (OHSU) School of Medicine, Portland, OR 97201, USA; Department of Pathology, OHSU School of Medicine, Portland, OR, USA; Cancer Biology Research Program, Knight Cancer Institute, OHSU School of Medicine, Portland, OR 97201, USA., Sears RC; Department of Molecular and Medical Genetics, OHSU School of Medicine, Portland, OR 97201, USA; Brenden-Colson Center for Pancreatic Care, OHSU School of Medicine, Portland, OR 97201, USA; Cancer Biology Research Program, Knight Cancer Institute, OHSU School of Medicine, Portland, OR 97201, USA., Kim J; Cancer Early Detection Advanced Research Center at Knight Cancer Institute, Oregon Health & Science University (OHSU) School of Medicine, Portland, OR 97201, USA; Department of Molecular and Medical Genetics, OHSU School of Medicine, Portland, OR 97201, USA; Cancer Biology Research Program, Knight Cancer Institute, OHSU School of Medicine, Portland, OR 97201, USA. Electronic address: kimjungs@ohsu.edu.
Source: STAR protocols [STAR Protoc] 2025 Mar 21; Vol. 6 (1), pp. 103672. Date of Electronic Publication: 2025 Mar 05.
Publication Type: Journal Article
Journal Info: Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE
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  Data: <searchLink fieldCode="AU" term="%22Tessier+J%22">Tessier J</searchLink>; Cancer Early Detection Advanced Research Center at Knight Cancer Institute, Oregon Health & Science University (OHSU) School of Medicine, Portland, OR 97201, USA.<br /><searchLink fieldCode="AU" term="%22Grygoryev+D%22">Grygoryev D</searchLink>; Cancer Early Detection Advanced Research Center at Knight Cancer Institute, Oregon Health & Science University (OHSU) School of Medicine, Portland, OR 97201, USA; Department of Molecular and Medical Genetics, OHSU School of Medicine, Portland, OR 97201, USA.<br /><searchLink fieldCode="AU" term="%22Chow-Castro+M%22">Chow-Castro M</searchLink>; Cancer Early Detection Advanced Research Center at Knight Cancer Institute, Oregon Health & Science University (OHSU) School of Medicine, Portland, OR 97201, USA.<br /><searchLink fieldCode="AU" term="%22Ekstrom+T%22">Ekstrom T</searchLink>; Cancer Early Detection Advanced Research Center at Knight Cancer Institute, Oregon Health & Science University (OHSU) School of Medicine, Portland, OR 97201, USA.<br /><searchLink fieldCode="AU" term="%22Manalo+E%22">Manalo E</searchLink>; Cancer Early Detection Advanced Research Center at Knight Cancer Institute, Oregon Health & Science University (OHSU) School of Medicine, Portland, OR 97201, USA.<br /><searchLink fieldCode="AU" term="%22Lee+SW%22">Lee SW</searchLink>; Cancer Early Detection Advanced Research Center at Knight Cancer Institute, Oregon Health & Science University (OHSU) School of Medicine, Portland, OR 97201, USA; Department of Molecular and Medical Genetics, OHSU School of Medicine, Portland, OR 97201, USA.<br /><searchLink fieldCode="AU" term="%22Berry+M%22">Berry M</searchLink>; Cancer Early Detection Advanced Research Center at Knight Cancer Institute, Oregon Health & Science University (OHSU) School of Medicine, Portland, OR 97201, USA.<br /><searchLink fieldCode="AU" term="%22Link+JM%22">Link JM</searchLink>; Department of Molecular and Medical Genetics, OHSU School of Medicine, Portland, OR 97201, USA; Brenden-Colson Center for Pancreatic Care, OHSU School of Medicine, Portland, OR 97201, USA.<br /><searchLink fieldCode="AU" term="%22Keith+D%22">Keith D</searchLink>; Brenden-Colson Center for Pancreatic Care, OHSU School of Medicine, Portland, OR 97201, USA.<br /><searchLink fieldCode="AU" term="%22Allen-Petersen+BL%22">Allen-Petersen BL</searchLink>; Department of Molecular and Medical Genetics, OHSU School of Medicine, Portland, OR 97201, USA; Brenden-Colson Center for Pancreatic Care, OHSU School of Medicine, Portland, OR 97201, USA.<br /><searchLink fieldCode="AU" term="%22Sheppard+B%22">Sheppard B</searchLink>; Brenden-Colson Center for Pancreatic Care, OHSU School of Medicine, Portland, OR 97201, USA; Department of Surgery, OHSU School of Medicine, Portland, OR 97201, USA.<br /><searchLink fieldCode="AU" term="%22Morgan+T%22">Morgan T</searchLink>; Cancer Early Detection Advanced Research Center at Knight Cancer Institute, Oregon Health & Science University (OHSU) School of Medicine, Portland, OR 97201, USA; Department of Pathology, OHSU School of Medicine, Portland, OR, USA; Cancer Biology Research Program, Knight Cancer Institute, OHSU School of Medicine, Portland, OR 97201, USA.<br /><searchLink fieldCode="AU" term="%22Sears+RC%22">Sears RC</searchLink>; Department of Molecular and Medical Genetics, OHSU School of Medicine, Portland, OR 97201, USA; Brenden-Colson Center for Pancreatic Care, OHSU School of Medicine, Portland, OR 97201, USA; Cancer Biology Research Program, Knight Cancer Institute, OHSU School of Medicine, Portland, OR 97201, USA.<br /><searchLink fieldCode="AU" term="%22Kim+J%22">Kim J</searchLink>; Cancer Early Detection Advanced Research Center at Knight Cancer Institute, Oregon Health & Science University (OHSU) School of Medicine, Portland, OR 97201, USA; Department of Molecular and Medical Genetics, OHSU School of Medicine, Portland, OR 97201, USA; Cancer Biology Research Program, Knight Cancer Institute, OHSU School of Medicine, Portland, OR 97201, USA. Electronic address: kimjungs@ohsu.edu.
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