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Streamlined and efficient genome editing in Cupriavidus necator H16 using an optimised SIBR-Cas system.

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Title: Streamlined and efficient genome editing in Cupriavidus necator H16 using an optimised SIBR-Cas system.
Authors: Della Valle S; Department of Engineering Science, University of Oxford, Oxford, UK., Orsi E; The Novo Nordisk Foundation Centre for Biosustainability, Technical University of Denmark, Lyngby, Denmark., Creutzburg SCA; Laboratory of Microbiology, Wageningen University and Research, Wageningen, The Netherlands., Jansen LFM; The Novo Nordisk Foundation Centre for Biosustainability, Technical University of Denmark, Lyngby, Denmark., Pentari EN; The Novo Nordisk Foundation Centre for Biosustainability, Technical University of Denmark, Lyngby, Denmark., Beisel CL; Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI), Würzburg, Germany; Medical Faculty, University of Würzburg, Würzburg, Germany., Steel H; Department of Engineering Science, University of Oxford, Oxford, UK., Nikel PI; The Novo Nordisk Foundation Centre for Biosustainability, Technical University of Denmark, Lyngby, Denmark., Staals RHJ; Laboratory of Microbiology, Wageningen University and Research, Wageningen, The Netherlands., Claassens NJ; Laboratory of Microbiology, Wageningen University and Research, Wageningen, The Netherlands., van der Oost J; Laboratory of Microbiology, Wageningen University and Research, Wageningen, The Netherlands., Huang WE; Department of Engineering Science, University of Oxford, Oxford, UK. Electronic address: wei.huang@eng.ox.ac.uk., Patinios C; Laboratory of Microbiology, Wageningen University and Research, Wageningen, The Netherlands; Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI), Würzburg, Germany; LSC-EMBL Partnership Institute for Genome Editing Technologies, Life Sciences Center, Vilnius University, Vilnius, Lithuania. Electronic address: constantinos.patinios@gmc.vu.lt.
Source: Trends in biotechnology [Trends Biotechnol] 2025 Jun; Vol. 43 (6), pp. 1470-1491. Date of Electronic Publication: 2025 Mar 13.
Publication Type: Journal Article; Research Support, Non-U.S. Gov't
Journal Info: Publisher: Elsevier Science Publishers Country of Publication: England NLM ID: 8310903 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3096 (Electronic) Linking ISSN: 01677799 NLM ISO Abbreviation: Trends Biotechnol Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: Streamlined and efficient genome editing in Cupriavidus necator H16 using an optimised SIBR-Cas system.
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  Data: <searchLink fieldCode="AU" term="%22Della+Valle+S%22">Della Valle S</searchLink>; Department of Engineering Science, University of Oxford, Oxford, UK.<br /><searchLink fieldCode="AU" term="%22Orsi+E%22">Orsi E</searchLink>; The Novo Nordisk Foundation Centre for Biosustainability, Technical University of Denmark, Lyngby, Denmark.<br /><searchLink fieldCode="AU" term="%22Creutzburg+SCA%22">Creutzburg SCA</searchLink>; Laboratory of Microbiology, Wageningen University and Research, Wageningen, The Netherlands.<br /><searchLink fieldCode="AU" term="%22Jansen+LFM%22">Jansen LFM</searchLink>; The Novo Nordisk Foundation Centre for Biosustainability, Technical University of Denmark, Lyngby, Denmark.<br /><searchLink fieldCode="AU" term="%22Pentari+EN%22">Pentari EN</searchLink>; The Novo Nordisk Foundation Centre for Biosustainability, Technical University of Denmark, Lyngby, Denmark.<br /><searchLink fieldCode="AU" term="%22Beisel+CL%22">Beisel CL</searchLink>; Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI), Würzburg, Germany; Medical Faculty, University of Würzburg, Würzburg, Germany.<br /><searchLink fieldCode="AU" term="%22Steel+H%22">Steel H</searchLink>; Department of Engineering Science, University of Oxford, Oxford, UK.<br /><searchLink fieldCode="AU" term="%22Nikel+PI%22">Nikel PI</searchLink>; The Novo Nordisk Foundation Centre for Biosustainability, Technical University of Denmark, Lyngby, Denmark.<br /><searchLink fieldCode="AU" term="%22Staals+RHJ%22">Staals RHJ</searchLink>; Laboratory of Microbiology, Wageningen University and Research, Wageningen, The Netherlands.<br /><searchLink fieldCode="AU" term="%22Claassens+NJ%22">Claassens NJ</searchLink>; Laboratory of Microbiology, Wageningen University and Research, Wageningen, The Netherlands.<br /><searchLink fieldCode="AU" term="%22van+der+Oost+J%22">van der Oost J</searchLink>; Laboratory of Microbiology, Wageningen University and Research, Wageningen, The Netherlands.<br /><searchLink fieldCode="AU" term="%22Huang+WE%22">Huang WE</searchLink>; Department of Engineering Science, University of Oxford, Oxford, UK. Electronic address: wei.huang@eng.ox.ac.uk.<br /><searchLink fieldCode="AU" term="%22Patinios+C%22">Patinios C</searchLink>; Laboratory of Microbiology, Wageningen University and Research, Wageningen, The Netherlands; Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI), Würzburg, Germany; LSC-EMBL Partnership Institute for Genome Editing Technologies, Life Sciences Center, Vilnius University, Vilnius, Lithuania. Electronic address: constantinos.patinios@gmc.vu.lt.
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  Data: <searchLink fieldCode="JN" term="%228310903%22">Trends in biotechnology</searchLink> [Trends Biotechnol] 2025 Jun; Vol. 43 (6), pp. 1470-1491. <i>Date of Electronic Publication: </i>2025 Mar 13.
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