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Fluorogenic, Subsingle-Turnover Monitoring of Enzymatic Reactions Involving NAD(P)H Provides a Generalized Platform for Directed Ultrahigh-Throughput Evolution of Biocatalysts in Microdroplets.

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Title: Fluorogenic, Subsingle-Turnover Monitoring of Enzymatic Reactions Involving NAD(P)H Provides a Generalized Platform for Directed Ultrahigh-Throughput Evolution of Biocatalysts in Microdroplets.
Authors: Penner M; Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K., Klein OJ; Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K.; Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K., Gantz M; Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K., Nintzel FEH; Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K., Prowald AC; Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K., Boss S; Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K., Barker P; Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K., Dupree P; Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K., Hollfelder F; Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K.
Source: Journal of the American Chemical Society [J Am Chem Soc] 2025 Apr 02; Vol. 147 (13), pp. 10903-10915. Date of Electronic Publication: 2025 Mar 24.
Publication Type: Journal Article; Research Support, Non-U.S. Gov't
Journal Info: Publisher: American Chemical Society Country of Publication: United States NLM ID: 7503056 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5126 (Electronic) Linking ISSN: 00027863 NLM ISO Abbreviation: J Am Chem Soc Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: Fluorogenic, Subsingle-Turnover Monitoring of Enzymatic Reactions Involving NAD(P)H Provides a Generalized Platform for Directed Ultrahigh-Throughput Evolution of Biocatalysts in Microdroplets.
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  Data: <searchLink fieldCode="AU" term="%22Penner+M%22">Penner M</searchLink>; Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K.<br /><searchLink fieldCode="AU" term="%22Klein+OJ%22">Klein OJ</searchLink>; Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K.; Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K.<br /><searchLink fieldCode="AU" term="%22Gantz+M%22">Gantz M</searchLink>; Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K.<br /><searchLink fieldCode="AU" term="%22Nintzel+FEH%22">Nintzel FEH</searchLink>; Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K.<br /><searchLink fieldCode="AU" term="%22Prowald+AC%22">Prowald AC</searchLink>; Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K.<br /><searchLink fieldCode="AU" term="%22Boss+S%22">Boss S</searchLink>; Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K.<br /><searchLink fieldCode="AU" term="%22Barker+P%22">Barker P</searchLink>; Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K.<br /><searchLink fieldCode="AU" term="%22Dupree+P%22">Dupree P</searchLink>; Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K.<br /><searchLink fieldCode="AU" term="%22Hollfelder+F%22">Hollfelder F</searchLink>; Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K.
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  Data: <searchLink fieldCode="JN" term="%227503056%22">Journal of the American Chemical Society</searchLink> [J Am Chem Soc] 2025 Apr 02; Vol. 147 (13), pp. 10903-10915. <i>Date of Electronic Publication: </i>2025 Mar 24.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22American+Chemical+Society%22">American Chemical Society </searchLink><i>Country of Publication: </i>United States <i>NLM ID: </i>7503056 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1520-5126 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2200027863%22">00027863 </searchLink><i>NLM ISO Abbreviation: </i>J Am Chem Soc <i>Subsets: </i>MEDLINE
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        Value: 10.1021/jacs.4c11804
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              Text: 2025 Apr 02
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