Fluorogenic, Subsingle-Turnover Monitoring of Enzymatic Reactions Involving NAD(P)H Provides a Generalized Platform for Directed Ultrahigh-Throughput Evolution of Biocatalysts in Microdroplets.
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| Title: | Fluorogenic, Subsingle-Turnover Monitoring of Enzymatic Reactions Involving NAD(P)H Provides a Generalized Platform for Directed Ultrahigh-Throughput Evolution of Biocatalysts in Microdroplets. |
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| Authors: | Penner M; Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K., Klein OJ; Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K.; Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K., Gantz M; Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K., Nintzel FEH; Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K., Prowald AC; Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K., Boss S; Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K., Barker P; Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K., Dupree P; Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K., Hollfelder F; Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K. |
| Source: | Journal of the American Chemical Society [J Am Chem Soc] 2025 Apr 02; Vol. 147 (13), pp. 10903-10915. Date of Electronic Publication: 2025 Mar 24. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Journal Info: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 7503056 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5126 (Electronic) Linking ISSN: 00027863 NLM ISO Abbreviation: J Am Chem Soc Subsets: MEDLINE |
| Database: | MEDLINE Ultimate |
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