Assessing the impact of cell isolation method on B cell gene expression using next-generation sequencing.

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Title: Assessing the impact of cell isolation method on B cell gene expression using next-generation sequencing.
Authors: Henning AN; Department of Transfusion Medicine and Center for Cellular Engineering, Clinical Center, National Institutes of Health, Bethesda, MD., Pardoe J; Department of Transfusion Medicine and Center for Cellular Engineering, Clinical Center, National Institutes of Health, Bethesda, MD., Owusu-Ansah D; Department of Transfusion Medicine and Center for Cellular Engineering, Clinical Center, National Institutes of Health, Bethesda, MD., Lei H; Department of Transfusion Medicine and Center for Cellular Engineering, Clinical Center, National Institutes of Health, Bethesda, MD., Robichaux K; Department of Transfusion Medicine and Center for Cellular Engineering, Clinical Center, National Institutes of Health, Bethesda, MD., Perinet L; Department of Transfusion Medicine and Center for Cellular Engineering, Clinical Center, National Institutes of Health, Bethesda, MD., Muccilli S; Department of Transfusion Medicine and Center for Cellular Engineering, Clinical Center, National Institutes of Health, Bethesda, MD., Highfill SL; Department of Transfusion Medicine and Center for Cellular Engineering, Clinical Center, National Institutes of Health, Bethesda, MD., De Giorgi V; Department of Transfusion Medicine and Center for Cellular Engineering, Clinical Center, National Institutes of Health, Bethesda, MD. Electronic address: valeria.degiorgi@nih.gov.
Source: Experimental hematology [Exp Hematol] 2025 Jun; Vol. 146, pp. 104766. Date of Electronic Publication: 2025 Mar 29.
Publication Type: Journal Article
Journal Info: Publisher: Elsevier Science Inc Country of Publication: Netherlands NLM ID: 0402313 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2399 (Electronic) Linking ISSN: 0301472X NLM ISO Abbreviation: Exp Hematol Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: <searchLink fieldCode="AU" term="%22Henning+AN%22">Henning AN</searchLink>; Department of Transfusion Medicine and Center for Cellular Engineering, Clinical Center, National Institutes of Health, Bethesda, MD.<br /><searchLink fieldCode="AU" term="%22Pardoe+J%22">Pardoe J</searchLink>; Department of Transfusion Medicine and Center for Cellular Engineering, Clinical Center, National Institutes of Health, Bethesda, MD.<br /><searchLink fieldCode="AU" term="%22Owusu-Ansah+D%22">Owusu-Ansah D</searchLink>; Department of Transfusion Medicine and Center for Cellular Engineering, Clinical Center, National Institutes of Health, Bethesda, MD.<br /><searchLink fieldCode="AU" term="%22Lei+H%22">Lei H</searchLink>; Department of Transfusion Medicine and Center for Cellular Engineering, Clinical Center, National Institutes of Health, Bethesda, MD.<br /><searchLink fieldCode="AU" term="%22Robichaux+K%22">Robichaux K</searchLink>; Department of Transfusion Medicine and Center for Cellular Engineering, Clinical Center, National Institutes of Health, Bethesda, MD.<br /><searchLink fieldCode="AU" term="%22Perinet+L%22">Perinet L</searchLink>; Department of Transfusion Medicine and Center for Cellular Engineering, Clinical Center, National Institutes of Health, Bethesda, MD.<br /><searchLink fieldCode="AU" term="%22Muccilli+S%22">Muccilli S</searchLink>; Department of Transfusion Medicine and Center for Cellular Engineering, Clinical Center, National Institutes of Health, Bethesda, MD.<br /><searchLink fieldCode="AU" term="%22Highfill+SL%22">Highfill SL</searchLink>; Department of Transfusion Medicine and Center for Cellular Engineering, Clinical Center, National Institutes of Health, Bethesda, MD.<br /><searchLink fieldCode="AU" term="%22De+Giorgi+V%22">De Giorgi V</searchLink>; Department of Transfusion Medicine and Center for Cellular Engineering, Clinical Center, National Institutes of Health, Bethesda, MD. Electronic address: valeria.degiorgi@nih.gov.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Elsevier+Science+Inc%22">Elsevier Science Inc </searchLink><i>Country of Publication: </i>Netherlands <i>NLM ID: </i>0402313 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1873-2399 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%220301472X%22">0301472X </searchLink><i>NLM ISO Abbreviation: </i>Exp Hematol <i>Subsets: </i>MEDLINE
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        Value: 10.1016/j.exphem.2025.104766
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        Text: English
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              Text: 2025 Jun
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