Development of a PCR-dipstick DNA chromatography-based tool for the detection of CTX-M- and TEM-producing Escherichia coli and Klebsiella pneumoniae isolated from patients in Kafue and Katete districts of Zambia.
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| Title: | Development of a PCR-dipstick DNA chromatography-based tool for the detection of CTX-M- and TEM-producing Escherichia coli and Klebsiella pneumoniae isolated from patients in Kafue and Katete districts of Zambia. |
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| Authors: | Shawa M; Hokudai Center for Zoonosis Control in Zambia, Hokkaido University, Lusaka, 10101, Zambia. misheckshawa@czc.hokudai.ac.jp.; Division of Infection and Immunity, International Institute for Zoonosis Control, Hokkaido University, N20 W10, Kita-Ku, Sapporo, 001-0020, Japan. misheckshawa@czc.hokudai.ac.jp.; Division of International Research Promotion, International Institute for Zoonosis Control, Hokkaido University, N20 W10, Kita-Ku, Sapporo, 001-0020, Japan. misheckshawa@czc.hokudai.ac.jp., Hayashida K; Hokudai Center for Zoonosis Control in Zambia, Hokkaido University, Lusaka, 10101, Zambia.; Division of Collaboration and Education, International Institute for Zoonosis Control, Hokkaido University, N20 W10, Kita-Ku, Sapporo, 001-0020, Japan., Nao N; Hokudai Center for Zoonosis Control in Zambia, Hokkaido University, Lusaka, 10101, Zambia.; Division of International Research Promotion, International Institute for Zoonosis Control, Hokkaido University, N20 W10, Kita-Ku, Sapporo, 001-0020, Japan.; One Health Research Center, Hokkaido University, N18 W9, Kita-Ku, Sapporo, 060-0818, Japan., Paudel A; GenEndeavor LLC, 26219 Eden Landing Rd, Hayward, CA, 94545, USA., Kamboyi H; Division of Infection and Immunity, International Institute for Zoonosis Control, Hokkaido University, N20 W10, Kita-Ku, Sapporo, 001-0020, Japan., Chambaro H; Central Veterinary Research Institute, Ministry of Fisheries and Livestock, Lusaka, 10101, Zambia., Mulavu M; Department of Biomedical Sciences, School of Health Sciences, University of Zambia, Lusaka, 10101, Zambia., Khumalo CS; Department of Biological Sciences, School of Veterinary Medicine, University of Zambia, Lusaka, 10101, Zambia., Nundwe M; Institute of Basic and Biomedical Sciences, Levy Mwanawasa Medical University, 10101, Lusaka, Zambia., Zorigt T; Division of Infection and Immunity, International Institute for Zoonosis Control, Hokkaido University, N20 W10, Kita-Ku, Sapporo, 001-0020, Japan., Nakamura C; Hokudai Center for Zoonosis Control in Zambia, Hokkaido University, Lusaka, 10101, Zambia.; Division of International Research Promotion, International Institute for Zoonosis Control, Hokkaido University, N20 W10, Kita-Ku, Sapporo, 001-0020, Japan., Qiu Y; Hokudai Center for Zoonosis Control in Zambia, Hokkaido University, Lusaka, 10101, Zambia.; Department of Virology-I, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku, Tokyo, 162-8640, Japan.; Management Department of Biosafety, Laboratory Animal, and Pathogen Bank, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku, Tokyo, 162-8640, Japan., Kawai N; Division of Collaboration and Education, International Institute for Zoonosis Control, Hokkaido University, N20 W10, Kita-Ku, Sapporo, 001-0020, Japan., Kasanga M; Department of Pathology and Microbiology, University Teaching Hospital, Lusaka, 10101, Zambia., Chizimu J; Zambia National Public Health Institute, Ministry of Health, Lusaka, 10101, Zambia.; Division of Bioresources, International Institute for Zoonosis Control, Hokkaido University, N20 W10, Kita-Ku, Sapporo, 001-0020, Japan., Moonga L; Department of Para-Clinical Studies, School of Veterinary Medicine, University of Zambia, Lusaka, 10101, Zambia., Ndebe J; Department of Disease Control, School of Veterinary Medicine, University of Zambia, Lusaka, 10101, Zambia., Simbotwe M; Department of Disease Control, School of Veterinary Medicine, University of Zambia, Lusaka, 10101, Zambia., Ogata S; Hokudai Center for Zoonosis Control in Zambia, Hokkaido University, Lusaka, 10101, Zambia.; Division of International Research Promotion, International Institute for Zoonosis Control, Hokkaido University, N20 W10, Kita-Ku, Sapporo, 001-0020, Japan., Samutela M; Department of Biomedical Sciences, School of Health Sciences, University of Zambia, Lusaka, 10101, Zambia., Nakajima C; Division of Bioresources, International Institute for Zoonosis Control, Hokkaido University, N20 W10, Kita-Ku, Sapporo, 001-0020, Japan., Chilengi R; Zambia National Public Health Institute, Ministry of Health, Lusaka, 10101, Zambia., Mutengo M; Institute of Basic and Biomedical Sciences, Levy Mwanawasa Medical University, 10101, Lusaka, Zambia., Kajihara M; Hokudai Center for Zoonosis Control in Zambia, Hokkaido University, Lusaka, 10101, Zambia.; Division of International Research Promotion, International Institute for Zoonosis Control, Hokkaido University, N20 W10, Kita-Ku, Sapporo, 001-0020, Japan., Sawa H; Hokudai Center for Zoonosis Control in Zambia, Hokkaido University, Lusaka, 10101, Zambia.; One Health Research Center, Hokkaido University, N18 W9, Kita-Ku, Sapporo, 060-0818, Japan.; Department of Disease Control, School of Veterinary Medicine, University of Zambia, Lusaka, 10101, Zambia.; International Collaboration Unit, International Institute for Zoonosis Control, Hokkaido University, N20 W10, Kita-Ku, Sapporo, 001-0020, Japan.; Institute for Vaccine Research and Development (HU-IVReD), Hokkaido University, N21 W11, Kita-Ku, Sapporo, 001-0021, Japan.; Division of Molecular Pathobiology, International Institute for Zoonosis Control, Hokkaido University, N20 W10, Kita-Ku, Sapporo, 001-0020, Japan.; Africa Centre of Excellence for Infectious Diseases of Humans and Animals, School of Veterinary Medicine, University of Zambia, Lusaka, 10101, Zambia.; Global Virus Network, 725 W Lombard Street, Baltimore, MD, 21201, USA., Hang'ombe B; Department of Para-Clinical Studies, School of Veterinary Medicine, University of Zambia, Lusaka, 10101, Zambia.; Africa Centre of Excellence for Infectious Diseases of Humans and Animals, School of Veterinary Medicine, University of Zambia, Lusaka, 10101, Zambia., Suzuki Y; Division of Bioresources, International Institute for Zoonosis Control, Hokkaido University, N20 W10, Kita-Ku, Sapporo, 001-0020, Japan.; Institute for Vaccine Research and Development (HU-IVReD), Hokkaido University, N21 W11, Kita-Ku, Sapporo, 001-0021, Japan., Higashi H; Division of Infection and Immunity, International Institute for Zoonosis Control, Hokkaido University, N20 W10, Kita-Ku, Sapporo, 001-0020, Japan. |
| Source: | BMC infectious diseases [BMC Infect Dis] 2025 Apr 16; Vol. 25 (1), pp. 541. Date of Electronic Publication: 2025 Apr 16. |
| Publication Type: | Journal Article |
| Journal Info: | Publisher: BioMed Central Country of Publication: England NLM ID: 100968551 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2334 (Electronic) Linking ISSN: 14712334 NLM ISO Abbreviation: BMC Infect Dis Subsets: MEDLINE |
| Database: | MEDLINE Ultimate |
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| ISSN: | 1471-2334 |
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| DOI: | 10.1186/s12879-025-10628-9 |
