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Machine learning-led semi-automated medium optimization reveals salt as key for flaviolin production in Pseudomonas putida.

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Title: Machine learning-led semi-automated medium optimization reveals salt as key for flaviolin production in Pseudomonas putida.
Authors: Zournas A; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Department of Energy Agile BioFoundry, Emeryville, CA, 94608, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA., Incha MR; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Department of Energy Agile BioFoundry, Emeryville, CA, 94608, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA., Radivojevic T; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Department of Energy Agile BioFoundry, Emeryville, CA, 94608, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA., Blay V; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA., Martí JM; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Department of Energy Agile BioFoundry, Emeryville, CA, 94608, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA., Costello Z; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Department of Energy Agile BioFoundry, Emeryville, CA, 94608, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA., Schmidt M; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA., Chung T; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA.; Department of Bioengineering, University of California, Berkeley, CA, 94720, USA., Thompson MG; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA., Pearson A; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA., Kinnunen PC; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Department of Energy Agile BioFoundry, Emeryville, CA, 94608, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA., Eng T; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA., Lawson CE; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA., Tan S; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Department of Energy Agile BioFoundry, Emeryville, CA, 94608, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA., Ogorzalek T; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Department of Energy Agile BioFoundry, Emeryville, CA, 94608, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA., Kaplan N; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Department of Energy Agile BioFoundry, Emeryville, CA, 94608, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA., Forrer M; Department of Energy Agile BioFoundry, Emeryville, CA, 94608, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA.; Biomaterials and Biomanufacturing, Sandia National Laboratories, Livermore, CA, 94550, USA., Backman T; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA., Mukhopadhyay A; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA., Hillson NJ; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Department of Energy Agile BioFoundry, Emeryville, CA, 94608, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA., Keasling JD; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA.; Department of Bioengineering, University of California, Berkeley, CA, 94720, USA.; Department of Chemical & Biomolecular Engineering, University of California, Berkeley, CA, 94720, USA., Garcia Martin H; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA. hgmartin@lbl.gov.; Department of Energy Agile BioFoundry, Emeryville, CA, 94608, USA. hgmartin@lbl.gov.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA. hgmartin@lbl.gov.; BCAM, Basque Center for Applied Mathematics, Bilbao, 48009, Spain. hgmartin@lbl.gov.
Source: Communications biology [Commun Biol] 2025 Apr 18; Vol. 8 (1), pp. 630. Date of Electronic Publication: 2025 Apr 18.
Publication Type: Journal Article
Journal Info: Publisher: Nature Publishing Group UK Country of Publication: England NLM ID: 101719179 Publication Model: Electronic Cited Medium: Internet ISSN: 2399-3642 (Electronic) Linking ISSN: 23993642 NLM ISO Abbreviation: Commun Biol Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: Machine learning-led semi-automated medium optimization reveals salt as key for flaviolin production in Pseudomonas putida.
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  Data: <searchLink fieldCode="AU" term="%22Zournas+A%22">Zournas A</searchLink>; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Department of Energy Agile BioFoundry, Emeryville, CA, 94608, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA.<br /><searchLink fieldCode="AU" term="%22Incha+MR%22">Incha MR</searchLink>; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Department of Energy Agile BioFoundry, Emeryville, CA, 94608, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA.<br /><searchLink fieldCode="AU" term="%22Radivojevic+T%22">Radivojevic T</searchLink>; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Department of Energy Agile BioFoundry, Emeryville, CA, 94608, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA.<br /><searchLink fieldCode="AU" term="%22Blay+V%22">Blay V</searchLink>; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA.<br /><searchLink fieldCode="AU" term="%22Martí+JM%22">Martí JM</searchLink>; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Department of Energy Agile BioFoundry, Emeryville, CA, 94608, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA.<br /><searchLink fieldCode="AU" term="%22Costello+Z%22">Costello Z</searchLink>; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Department of Energy Agile BioFoundry, Emeryville, CA, 94608, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA.<br /><searchLink fieldCode="AU" term="%22Schmidt+M%22">Schmidt M</searchLink>; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA.<br /><searchLink fieldCode="AU" term="%22Chung+T%22">Chung T</searchLink>; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA.; Department of Bioengineering, University of California, Berkeley, CA, 94720, USA.<br /><searchLink fieldCode="AU" term="%22Thompson+MG%22">Thompson MG</searchLink>; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA.<br /><searchLink fieldCode="AU" term="%22Pearson+A%22">Pearson A</searchLink>; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA.<br /><searchLink fieldCode="AU" term="%22Kinnunen+PC%22">Kinnunen PC</searchLink>; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Department of Energy Agile BioFoundry, Emeryville, CA, 94608, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA.<br /><searchLink fieldCode="AU" term="%22Eng+T%22">Eng T</searchLink>; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA.<br /><searchLink fieldCode="AU" term="%22Lawson+CE%22">Lawson CE</searchLink>; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA.<br /><searchLink fieldCode="AU" term="%22Tan+S%22">Tan S</searchLink>; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Department of Energy Agile BioFoundry, Emeryville, CA, 94608, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA.<br /><searchLink fieldCode="AU" term="%22Ogorzalek+T%22">Ogorzalek T</searchLink>; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Department of Energy Agile BioFoundry, Emeryville, CA, 94608, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA.<br /><searchLink fieldCode="AU" term="%22Kaplan+N%22">Kaplan N</searchLink>; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Department of Energy Agile BioFoundry, Emeryville, CA, 94608, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA.<br /><searchLink fieldCode="AU" term="%22Forrer+M%22">Forrer M</searchLink>; Department of Energy Agile BioFoundry, Emeryville, CA, 94608, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA.; Biomaterials and Biomanufacturing, Sandia National Laboratories, Livermore, CA, 94550, USA.<br /><searchLink fieldCode="AU" term="%22Backman+T%22">Backman T</searchLink>; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA.<br /><searchLink fieldCode="AU" term="%22Mukhopadhyay+A%22">Mukhopadhyay A</searchLink>; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA.<br /><searchLink fieldCode="AU" term="%22Hillson+NJ%22">Hillson NJ</searchLink>; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Department of Energy Agile BioFoundry, Emeryville, CA, 94608, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA.<br /><searchLink fieldCode="AU" term="%22Keasling+JD%22">Keasling JD</searchLink>; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA.; Department of Bioengineering, University of California, Berkeley, CA, 94720, USA.; Department of Chemical & Biomolecular Engineering, University of California, Berkeley, CA, 94720, USA.<br /><searchLink fieldCode="AU" term="%22Garcia+Martin+H%22">Garcia Martin H</searchLink>; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA. hgmartin@lbl.gov.; Department of Energy Agile BioFoundry, Emeryville, CA, 94608, USA. hgmartin@lbl.gov.; Joint BioEnergy Institute, Emeryville, CA, 94608, USA. hgmartin@lbl.gov.; BCAM, Basque Center for Applied Mathematics, Bilbao, 48009, Spain. hgmartin@lbl.gov.
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  Data: <searchLink fieldCode="JN" term="%22101719179%22">Communications biology</searchLink> [Commun Biol] 2025 Apr 18; Vol. 8 (1), pp. 630. <i>Date of Electronic Publication: </i>2025 Apr 18.
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