Use of allele-specific qPCR and PCR-RFLP analysis for rapid detection of the SARS-CoV-2 variants in Tunisia: A cheap flexible approach adapted for developing countries.
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| Title: | Use of allele-specific qPCR and PCR-RFLP analysis for rapid detection of the SARS-CoV-2 variants in Tunisia: A cheap flexible approach adapted for developing countries. |
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| Authors: | Ayadi W; Laboratory of Molecular Biotechnology of Eukaryotes, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia., Smaoui F; Laboratory of Microbiology, Research Laboratory for Microorganisms and Human Disease LR03SP03, Habib Bourguiba University-Hospital, Sfax, Tunisia., Gargouri S; Laboratory of Microbiology, Research Laboratory for Microorganisms and Human Disease LR03SP03, Habib Bourguiba University-Hospital, Sfax, Tunisia.; Faculty of Medicine, University of Sfax, Sfax, Tunisia., Ferjani S; Charles Nicolle Hospital, Laboratory of Microbiology, Tunis, Tunisia.; University of Tunis El Manar, Faculty of Medicine of Tunis, Tunis, Tunisia., Hamzaoui Z; University of Tunis El Manar, Faculty of Medicine of Tunis, Tunis, Tunisia., Taktak A; Laboratory of Molecular Biotechnology of Eukaryotes, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia.; Laboratory of Microbiology, Research Laboratory for Microorganisms and Human Disease LR03SP03, Habib Bourguiba University-Hospital, Sfax, Tunisia., Chtourou A; Laboratory of Microbiology, Research Laboratory for Microorganisms and Human Disease LR03SP03, Habib Bourguiba University-Hospital, Sfax, Tunisia.; Faculty of Medicine, University of Sfax, Sfax, Tunisia., Skouri-Gargouri H; Laboratory of Molecular Biotechnology of Eukaryotes, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia., Sassi AH; Laboratory of Molecular Biotechnology of Eukaryotes, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia., Sassi MB; National Center Chalbi belkahia of Pharmacovigilance of Tunis, Laboratory of Clinical Pharmacology, Tunis, Tunisia.; University of Tunis El Manar, Faculty of Medicine of Tunis, Tunis, Tunisia., Trabelsi S; National Center Chalbi belkahia of Pharmacovigilance of Tunis, Laboratory of Clinical Pharmacology, Tunis, Tunisia.; University of Tunis El Manar, Faculty of Medicine of Tunis, Tunis, Tunisia., Gargouri A; Laboratory of Molecular Biotechnology of Eukaryotes, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia., Boubaker IB; Charles Nicolle Hospital, Laboratory of Microbiology, Tunis, Tunisia.; University of Tunis El Manar, Faculty of Medicine of Tunis, Tunis, Tunisia., Karray-Hakim H; Laboratory of Microbiology, Research Laboratory for Microorganisms and Human Disease LR03SP03, Habib Bourguiba University-Hospital, Sfax, Tunisia.; Faculty of Medicine, University of Sfax, Sfax, Tunisia., Mokdad-Gargouri R; Laboratory of Molecular Biotechnology of Eukaryotes, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia., Feki-Berrajah L; Laboratory of Microbiology, Research Laboratory for Microorganisms and Human Disease LR03SP03, Habib Bourguiba University-Hospital, Sfax, Tunisia.; Faculty of Medicine, University of Sfax, Sfax, Tunisia. |
| Source: | PloS one [PLoS One] 2025 May 05; Vol. 20 (5), pp. e0321581. Date of Electronic Publication: 2025 May 05 (Print Publication: 2025). |
| Publication Type: | Journal Article |
| Journal Info: | Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE |
| Database: | MEDLINE Ultimate |
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| ISSN: | 1932-6203 |
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| DOI: | 10.1371/journal.pone.0321581 |
