View in EDS HTML Full Text PDF Full Text

Use of allele-specific qPCR and PCR-RFLP analysis for rapid detection of the SARS-CoV-2 variants in Tunisia: A cheap flexible approach adapted for developing countries.

Saved in:
Bibliographic Details
Title: Use of allele-specific qPCR and PCR-RFLP analysis for rapid detection of the SARS-CoV-2 variants in Tunisia: A cheap flexible approach adapted for developing countries.
Authors: Ayadi W; Laboratory of Molecular Biotechnology of Eukaryotes, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia., Smaoui F; Laboratory of Microbiology, Research Laboratory for Microorganisms and Human Disease LR03SP03, Habib Bourguiba University-Hospital, Sfax, Tunisia., Gargouri S; Laboratory of Microbiology, Research Laboratory for Microorganisms and Human Disease LR03SP03, Habib Bourguiba University-Hospital, Sfax, Tunisia.; Faculty of Medicine, University of Sfax, Sfax, Tunisia., Ferjani S; Charles Nicolle Hospital, Laboratory of Microbiology, Tunis, Tunisia.; University of Tunis El Manar, Faculty of Medicine of Tunis, Tunis, Tunisia., Hamzaoui Z; University of Tunis El Manar, Faculty of Medicine of Tunis, Tunis, Tunisia., Taktak A; Laboratory of Molecular Biotechnology of Eukaryotes, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia.; Laboratory of Microbiology, Research Laboratory for Microorganisms and Human Disease LR03SP03, Habib Bourguiba University-Hospital, Sfax, Tunisia., Chtourou A; Laboratory of Microbiology, Research Laboratory for Microorganisms and Human Disease LR03SP03, Habib Bourguiba University-Hospital, Sfax, Tunisia.; Faculty of Medicine, University of Sfax, Sfax, Tunisia., Skouri-Gargouri H; Laboratory of Molecular Biotechnology of Eukaryotes, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia., Sassi AH; Laboratory of Molecular Biotechnology of Eukaryotes, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia., Sassi MB; National Center Chalbi belkahia of Pharmacovigilance of Tunis, Laboratory of Clinical Pharmacology, Tunis, Tunisia.; University of Tunis El Manar, Faculty of Medicine of Tunis, Tunis, Tunisia., Trabelsi S; National Center Chalbi belkahia of Pharmacovigilance of Tunis, Laboratory of Clinical Pharmacology, Tunis, Tunisia.; University of Tunis El Manar, Faculty of Medicine of Tunis, Tunis, Tunisia., Gargouri A; Laboratory of Molecular Biotechnology of Eukaryotes, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia., Boubaker IB; Charles Nicolle Hospital, Laboratory of Microbiology, Tunis, Tunisia.; University of Tunis El Manar, Faculty of Medicine of Tunis, Tunis, Tunisia., Karray-Hakim H; Laboratory of Microbiology, Research Laboratory for Microorganisms and Human Disease LR03SP03, Habib Bourguiba University-Hospital, Sfax, Tunisia.; Faculty of Medicine, University of Sfax, Sfax, Tunisia., Mokdad-Gargouri R; Laboratory of Molecular Biotechnology of Eukaryotes, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia., Feki-Berrajah L; Laboratory of Microbiology, Research Laboratory for Microorganisms and Human Disease LR03SP03, Habib Bourguiba University-Hospital, Sfax, Tunisia.; Faculty of Medicine, University of Sfax, Sfax, Tunisia.
Source: PloS one [PLoS One] 2025 May 05; Vol. 20 (5), pp. e0321581. Date of Electronic Publication: 2025 May 05 (Print Publication: 2025).
Publication Type: Journal Article
Journal Info: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
Database: MEDLINE Ultimate
Full text is not displayed to guests.
FullText Links:
  – Type: pdflink
Text:
  Availability: 1
Header DbId: mdl
DbLabel: MEDLINE Ultimate
An: 40323950
AccessLevel: 2
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Use of allele-specific qPCR and PCR-RFLP analysis for rapid detection of the SARS-CoV-2 variants in Tunisia: A cheap flexible approach adapted for developing countries.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AU" term="%22Ayadi+W%22">Ayadi W</searchLink>; Laboratory of Molecular Biotechnology of Eukaryotes, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia.<br /><searchLink fieldCode="AU" term="%22Smaoui+F%22">Smaoui F</searchLink>; Laboratory of Microbiology, Research Laboratory for Microorganisms and Human Disease LR03SP03, Habib Bourguiba University-Hospital, Sfax, Tunisia.<br /><searchLink fieldCode="AU" term="%22Gargouri+S%22">Gargouri S</searchLink>; Laboratory of Microbiology, Research Laboratory for Microorganisms and Human Disease LR03SP03, Habib Bourguiba University-Hospital, Sfax, Tunisia.; Faculty of Medicine, University of Sfax, Sfax, Tunisia.<br /><searchLink fieldCode="AU" term="%22Ferjani+S%22">Ferjani S</searchLink>; Charles Nicolle Hospital, Laboratory of Microbiology, Tunis, Tunisia.; University of Tunis El Manar, Faculty of Medicine of Tunis, Tunis, Tunisia.<br /><searchLink fieldCode="AU" term="%22Hamzaoui+Z%22">Hamzaoui Z</searchLink>; University of Tunis El Manar, Faculty of Medicine of Tunis, Tunis, Tunisia.<br /><searchLink fieldCode="AU" term="%22Taktak+A%22">Taktak A</searchLink>; Laboratory of Molecular Biotechnology of Eukaryotes, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia.; Laboratory of Microbiology, Research Laboratory for Microorganisms and Human Disease LR03SP03, Habib Bourguiba University-Hospital, Sfax, Tunisia.<br /><searchLink fieldCode="AU" term="%22Chtourou+A%22">Chtourou A</searchLink>; Laboratory of Microbiology, Research Laboratory for Microorganisms and Human Disease LR03SP03, Habib Bourguiba University-Hospital, Sfax, Tunisia.; Faculty of Medicine, University of Sfax, Sfax, Tunisia.<br /><searchLink fieldCode="AU" term="%22Skouri-Gargouri+H%22">Skouri-Gargouri H</searchLink>; Laboratory of Molecular Biotechnology of Eukaryotes, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia.<br /><searchLink fieldCode="AU" term="%22Sassi+AH%22">Sassi AH</searchLink>; Laboratory of Molecular Biotechnology of Eukaryotes, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia.<br /><searchLink fieldCode="AU" term="%22Sassi+MB%22">Sassi MB</searchLink>; National Center Chalbi belkahia of Pharmacovigilance of Tunis, Laboratory of Clinical Pharmacology, Tunis, Tunisia.; University of Tunis El Manar, Faculty of Medicine of Tunis, Tunis, Tunisia.<br /><searchLink fieldCode="AU" term="%22Trabelsi+S%22">Trabelsi S</searchLink>; National Center Chalbi belkahia of Pharmacovigilance of Tunis, Laboratory of Clinical Pharmacology, Tunis, Tunisia.; University of Tunis El Manar, Faculty of Medicine of Tunis, Tunis, Tunisia.<br /><searchLink fieldCode="AU" term="%22Gargouri+A%22">Gargouri A</searchLink>; Laboratory of Molecular Biotechnology of Eukaryotes, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia.<br /><searchLink fieldCode="AU" term="%22Boubaker+IB%22">Boubaker IB</searchLink>; Charles Nicolle Hospital, Laboratory of Microbiology, Tunis, Tunisia.; University of Tunis El Manar, Faculty of Medicine of Tunis, Tunis, Tunisia.<br /><searchLink fieldCode="AU" term="%22Karray-Hakim+H%22">Karray-Hakim H</searchLink>; Laboratory of Microbiology, Research Laboratory for Microorganisms and Human Disease LR03SP03, Habib Bourguiba University-Hospital, Sfax, Tunisia.; Faculty of Medicine, University of Sfax, Sfax, Tunisia.<br /><searchLink fieldCode="AU" term="%22Mokdad-Gargouri+R%22">Mokdad-Gargouri R</searchLink>; Laboratory of Molecular Biotechnology of Eukaryotes, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia.<br /><searchLink fieldCode="AU" term="%22Feki-Berrajah+L%22">Feki-Berrajah L</searchLink>; Laboratory of Microbiology, Research Laboratory for Microorganisms and Human Disease LR03SP03, Habib Bourguiba University-Hospital, Sfax, Tunisia.; Faculty of Medicine, University of Sfax, Sfax, Tunisia.
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22101285081%22">PloS one</searchLink> [PLoS One] 2025 May 05; Vol. 20 (5), pp. e0321581. <i>Date of Electronic Publication: </i>2025 May 05 (<i>Print Publication: </i>2025).
– Name: TypePub
  Label: Publication Type
  Group: TypPub
  Data: Journal Article
– Name: TitleSource
  Label: Journal Info
  Group: Src
  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Public+Library+of+Science%22">Public Library of Science </searchLink><i>Country of Publication: </i>United States <i>NLM ID: </i>101285081 <i>Publication Model: </i>eCollection <i>Cited Medium: </i>Internet <i>ISSN: </i>1932-6203 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2219326203%22">19326203 </searchLink><i>NLM ISO Abbreviation: </i>PLoS One <i>Subsets: </i>MEDLINE
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=mdl&AN=40323950
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1371/journal.pone.0321581
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        StartPage: e0321581
    Titles:
      – TitleFull: Use of allele-specific qPCR and PCR-RFLP analysis for rapid detection of the SARS-CoV-2 variants in Tunisia: A cheap flexible approach adapted for developing countries.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Ayadi W
      – PersonEntity:
          Name:
            NameFull: Smaoui F
      – PersonEntity:
          Name:
            NameFull: Gargouri S
      – PersonEntity:
          Name:
            NameFull: Ferjani S
      – PersonEntity:
          Name:
            NameFull: Hamzaoui Z
      – PersonEntity:
          Name:
            NameFull: Taktak A
      – PersonEntity:
          Name:
            NameFull: Chtourou A
      – PersonEntity:
          Name:
            NameFull: Skouri-Gargouri H
      – PersonEntity:
          Name:
            NameFull: Sassi AH
      – PersonEntity:
          Name:
            NameFull: Sassi MB
      – PersonEntity:
          Name:
            NameFull: Trabelsi S
      – PersonEntity:
          Name:
            NameFull: Gargouri A
      – PersonEntity:
          Name:
            NameFull: Boubaker IB
      – PersonEntity:
          Name:
            NameFull: Karray-Hakim H
      – PersonEntity:
          Name:
            NameFull: Mokdad-Gargouri R
      – PersonEntity:
          Name:
            NameFull: Feki-Berrajah L
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 05
              M: 05
              Text: 2025 May 05
              Type: published
              Y: 2025
          Identifiers:
            – Type: issn-electronic
              Value: 1932-6203
          Numbering:
            – Type: volume
              Value: 20
            – Type: issue
              Value: 5
          Titles:
            – TitleFull: PloS one
              Type: main
ResultId 1