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Evaluation of metatranscriptomic sequencing protocols to obtain full-length RNA virus genomes from mammalian tissues.

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Title: Evaluation of metatranscriptomic sequencing protocols to obtain full-length RNA virus genomes from mammalian tissues.
Authors: Li Y; Department of Microbiology, Immunology and Transplantation, KU Leuven, Rega Institute, KU Leuven, Leuven, Belgium.; Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China., Polychronopoulou M; Department of Hygiene, Epidemiology and Medical Statistics, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece., Boonen I; Department of Microbiology, Immunology and Transplantation, KU Leuven, Rega Institute, KU Leuven, Leuven, Belgium., Fikatas A; Department of Microbiology, Immunology and Transplantation, KU Leuven, Rega Institute, KU Leuven, Leuven, Belgium., Gryseels S; Evolutionary Ecology group (EVECO), Department of Biology, University of Antwerp, Antwerp, Belgium., Laudisoit A; Evolutionary Ecology group (EVECO), Department of Biology, University of Antwerp, Antwerp, Belgium.; EcoHealth Alliance, New York, New York, United States of America., de Bellocq JG; Institute of Vertebrate Biology, The Czech Academy of Sciences, Brno, Czech Republic., Vrancken B; Department of Microbiology, Immunology and Transplantation, KU Leuven, Rega Institute, KU Leuven, Leuven, Belgium.; Spatial Epidemiology Lab (SpELL), Université Libre de Bruxelles, Bruxelles, Belgium., Magiorkinis G; Department of Hygiene, Epidemiology and Medical Statistics, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece., Lemey P; Department of Microbiology, Immunology and Transplantation, KU Leuven, Rega Institute, KU Leuven, Leuven, Belgium., Bletsa M; Department of Microbiology, Immunology and Transplantation, KU Leuven, Rega Institute, KU Leuven, Leuven, Belgium.; Department of Hygiene, Epidemiology and Medical Statistics, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece.; Bioinformatics and Applied Genomics, Department of Microbiology, Hellenic Pasteur Institute, Athens, Greece.
Source: PloS one [PLoS One] 2025 May 30; Vol. 20 (5), pp. e0324537. Date of Electronic Publication: 2025 May 30 (Print Publication: 2025).
Publication Type: Journal Article
Journal Info: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: <searchLink fieldCode="AU" term="%22Li+Y%22">Li Y</searchLink>; Department of Microbiology, Immunology and Transplantation, KU Leuven, Rega Institute, KU Leuven, Leuven, Belgium.; Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China.<br /><searchLink fieldCode="AU" term="%22Polychronopoulou+M%22">Polychronopoulou M</searchLink>; Department of Hygiene, Epidemiology and Medical Statistics, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece.<br /><searchLink fieldCode="AU" term="%22Boonen+I%22">Boonen I</searchLink>; Department of Microbiology, Immunology and Transplantation, KU Leuven, Rega Institute, KU Leuven, Leuven, Belgium.<br /><searchLink fieldCode="AU" term="%22Fikatas+A%22">Fikatas A</searchLink>; Department of Microbiology, Immunology and Transplantation, KU Leuven, Rega Institute, KU Leuven, Leuven, Belgium.<br /><searchLink fieldCode="AU" term="%22Gryseels+S%22">Gryseels S</searchLink>; Evolutionary Ecology group (EVECO), Department of Biology, University of Antwerp, Antwerp, Belgium.<br /><searchLink fieldCode="AU" term="%22Laudisoit+A%22">Laudisoit A</searchLink>; Evolutionary Ecology group (EVECO), Department of Biology, University of Antwerp, Antwerp, Belgium.; EcoHealth Alliance, New York, New York, United States of America.<br /><searchLink fieldCode="AU" term="%22de+Bellocq+JG%22">de Bellocq JG</searchLink>; Institute of Vertebrate Biology, The Czech Academy of Sciences, Brno, Czech Republic.<br /><searchLink fieldCode="AU" term="%22Vrancken+B%22">Vrancken B</searchLink>; Department of Microbiology, Immunology and Transplantation, KU Leuven, Rega Institute, KU Leuven, Leuven, Belgium.; Spatial Epidemiology Lab (SpELL), Université Libre de Bruxelles, Bruxelles, Belgium.<br /><searchLink fieldCode="AU" term="%22Magiorkinis+G%22">Magiorkinis G</searchLink>; Department of Hygiene, Epidemiology and Medical Statistics, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece.<br /><searchLink fieldCode="AU" term="%22Lemey+P%22">Lemey P</searchLink>; Department of Microbiology, Immunology and Transplantation, KU Leuven, Rega Institute, KU Leuven, Leuven, Belgium.<br /><searchLink fieldCode="AU" term="%22Bletsa+M%22">Bletsa M</searchLink>; Department of Microbiology, Immunology and Transplantation, KU Leuven, Rega Institute, KU Leuven, Leuven, Belgium.; Department of Hygiene, Epidemiology and Medical Statistics, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece.; Bioinformatics and Applied Genomics, Department of Microbiology, Hellenic Pasteur Institute, Athens, Greece.
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