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Optimization of Metagenomic Next-Generation Sequencing Workflow with a Novel Host Depletion Method for Enhanced Pathogen Detection.

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Title: Optimization of Metagenomic Next-Generation Sequencing Workflow with a Novel Host Depletion Method for Enhanced Pathogen Detection.
Authors: Chen YC; Emergency Department, Taipei Veterans General Hospital, No. 201, Sec. 2, Shipai Rd., Beitou Dist., Taipei, 11217, Taiwan.; School of Medicine, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan.; Department of Emergency Medicine, School of Medicine, National Defence Medical Center, Taipei, 11490, Taiwan., Liao PH; Emergency Department, Taipei Veterans General Hospital, No. 201, Sec. 2, Shipai Rd., Beitou Dist., Taipei, 11217, Taiwan.; School of Medicine, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan., Chen YW; School of Medicine, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan.; Department of Chest Medicine, Taipei Veterans General Hospital, Taipei, Taiwan.; Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan., Yen DH; Emergency Department, Taipei Veterans General Hospital, No. 201, Sec. 2, Shipai Rd., Beitou Dist., Taipei, 11217, Taiwan.; School of Medicine, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan.; Department of Emergency Medicine, School of Medicine, National Defence Medical Center, Taipei, 11490, Taiwan., How CK; Emergency Department, Taipei Veterans General Hospital, No. 201, Sec. 2, Shipai Rd., Beitou Dist., Taipei, 11217, Taiwan.; School of Medicine, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan., Chang CM; Emergency Department, Taipei Veterans General Hospital, No. 201, Sec. 2, Shipai Rd., Beitou Dist., Taipei, 11217, Taiwan. cmchang5@vghtpe.gov.tw.; School of Medicine, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan. cmchang5@vghtpe.gov.tw.
Source: Molecular diagnosis & therapy [Mol Diagn Ther] 2025 Sep; Vol. 29 (5), pp. 689-699. Date of Electronic Publication: 2025 Jul 16.
Publication Type: Journal Article; Research Support, Non-U.S. Gov't
Journal Info: Publisher: Adis, Springer International Country of Publication: New Zealand NLM ID: 101264260 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1179-2000 (Electronic) Linking ISSN: 11771062 NLM ISO Abbreviation: Mol Diagn Ther Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: Optimization of Metagenomic Next-Generation Sequencing Workflow with a Novel Host Depletion Method for Enhanced Pathogen Detection.
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  Data: <searchLink fieldCode="AU" term="%22Chen+YC%22">Chen YC</searchLink>; Emergency Department, Taipei Veterans General Hospital, No. 201, Sec. 2, Shipai Rd., Beitou Dist., Taipei, 11217, Taiwan.; School of Medicine, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan.; Department of Emergency Medicine, School of Medicine, National Defence Medical Center, Taipei, 11490, Taiwan.<br /><searchLink fieldCode="AU" term="%22Liao+PH%22">Liao PH</searchLink>; Emergency Department, Taipei Veterans General Hospital, No. 201, Sec. 2, Shipai Rd., Beitou Dist., Taipei, 11217, Taiwan.; School of Medicine, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan.<br /><searchLink fieldCode="AU" term="%22Chen+YW%22">Chen YW</searchLink>; School of Medicine, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan.; Department of Chest Medicine, Taipei Veterans General Hospital, Taipei, Taiwan.; Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan.<br /><searchLink fieldCode="AU" term="%22Yen+DH%22">Yen DH</searchLink>; Emergency Department, Taipei Veterans General Hospital, No. 201, Sec. 2, Shipai Rd., Beitou Dist., Taipei, 11217, Taiwan.; School of Medicine, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan.; Department of Emergency Medicine, School of Medicine, National Defence Medical Center, Taipei, 11490, Taiwan.<br /><searchLink fieldCode="AU" term="%22How+CK%22">How CK</searchLink>; Emergency Department, Taipei Veterans General Hospital, No. 201, Sec. 2, Shipai Rd., Beitou Dist., Taipei, 11217, Taiwan.; School of Medicine, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan.<br /><searchLink fieldCode="AU" term="%22Chang+CM%22">Chang CM</searchLink>; Emergency Department, Taipei Veterans General Hospital, No. 201, Sec. 2, Shipai Rd., Beitou Dist., Taipei, 11217, Taiwan. cmchang5@vghtpe.gov.tw.; School of Medicine, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan. cmchang5@vghtpe.gov.tw.
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  Data: <searchLink fieldCode="JN" term="%22101264260%22">Molecular diagnosis & therapy</searchLink> [Mol Diagn Ther] 2025 Sep; Vol. 29 (5), pp. 689-699. <i>Date of Electronic Publication: </i>2025 Jul 16.
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  Data: Journal Article; Research Support, Non-U.S. Gov't
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Adis%2C+Springer+International%22">Adis, Springer International </searchLink><i>Country of Publication: </i>New Zealand <i>NLM ID: </i>101264260 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1179-2000 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2211771062%22">11771062 </searchLink><i>NLM ISO Abbreviation: </i>Mol Diagn Ther <i>Subsets: </i>MEDLINE
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        Value: 10.1007/s40291-025-00797-3
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      – Code: eng
        Text: English
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        StartPage: 689
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      – TitleFull: Optimization of Metagenomic Next-Generation Sequencing Workflow with a Novel Host Depletion Method for Enhanced Pathogen Detection.
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            NameFull: Chen YC
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            – D: 01
              M: 09
              Text: 2025 Sep
              Type: published
              Y: 2025
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              Value: 1179-2000
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              Value: 29
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              Value: 5
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