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Functional and epitope specific monoclonal antibody discovery directly from immune sera using cryo-EM.

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Title: Functional and epitope specific monoclonal antibody discovery directly from immune sera using cryo-EM.
Authors: Ferguson JA; Department of Integrative, Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA., Raghavan SSR; Department of Integrative, Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA., Alzua GP; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.; Center for Vaccine Research and Pandemic Preparedness (C-VaRPP), Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA., Bhavsar D; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.; Center for Vaccine Research and Pandemic Preparedness (C-VaRPP), Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA., Huang J; Department of Integrative, Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA., Rodriguez AJ; Department of Integrative, Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA., Torres JL; Department of Integrative, Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA., Bottermann M; The Ragon Institute of Mass General, MIT, and Harvard, Cambridge, MA 02139, USA., Han J; Department of Integrative, Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA., Krammer F; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.; Center for Vaccine Research and Pandemic Preparedness (C-VaRPP), Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.; Department of Pathology, Molecular and Cell-Based Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.; Ignaz Semmelweis Institute, Interuniversity Institute for Infection Research, Medical University of Vienna, Vienna, Austria., Batista FD; The Ragon Institute of Mass General, MIT, and Harvard, Cambridge, MA 02139, USA.; Biology Department, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Ward AB; Department of Integrative, Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Source: Science advances [Sci Adv] 2025 Aug 15; Vol. 11 (33), pp. eadv8257. Date of Electronic Publication: 2025 Aug 15.
Publication Type: Journal Article
Journal Info: Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 101653440 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2375-2548 (Electronic) Linking ISSN: 23752548 NLM ISO Abbreviation: Sci Adv Subsets: MEDLINE
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  Data: <searchLink fieldCode="AU" term="%22Ferguson+JA%22">Ferguson JA</searchLink>; Department of Integrative, Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.<br /><searchLink fieldCode="AU" term="%22Raghavan+SSR%22">Raghavan SSR</searchLink>; Department of Integrative, Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.<br /><searchLink fieldCode="AU" term="%22Alzua+GP%22">Alzua GP</searchLink>; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.; Center for Vaccine Research and Pandemic Preparedness (C-VaRPP), Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.<br /><searchLink fieldCode="AU" term="%22Bhavsar+D%22">Bhavsar D</searchLink>; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.; Center for Vaccine Research and Pandemic Preparedness (C-VaRPP), Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.<br /><searchLink fieldCode="AU" term="%22Huang+J%22">Huang J</searchLink>; Department of Integrative, Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.<br /><searchLink fieldCode="AU" term="%22Rodriguez+AJ%22">Rodriguez AJ</searchLink>; Department of Integrative, Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.<br /><searchLink fieldCode="AU" term="%22Torres+JL%22">Torres JL</searchLink>; Department of Integrative, Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.<br /><searchLink fieldCode="AU" term="%22Bottermann+M%22">Bottermann M</searchLink>; The Ragon Institute of Mass General, MIT, and Harvard, Cambridge, MA 02139, USA.<br /><searchLink fieldCode="AU" term="%22Han+J%22">Han J</searchLink>; Department of Integrative, Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.<br /><searchLink fieldCode="AU" term="%22Krammer+F%22">Krammer F</searchLink>; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.; Center for Vaccine Research and Pandemic Preparedness (C-VaRPP), Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.; Department of Pathology, Molecular and Cell-Based Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.; Ignaz Semmelweis Institute, Interuniversity Institute for Infection Research, Medical University of Vienna, Vienna, Austria.<br /><searchLink fieldCode="AU" term="%22Batista+FD%22">Batista FD</searchLink>; The Ragon Institute of Mass General, MIT, and Harvard, Cambridge, MA 02139, USA.; Biology Department, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.<br /><searchLink fieldCode="AU" term="%22Ward+AB%22">Ward AB</searchLink>; Department of Integrative, Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
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