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Paired plus-minus sequencing is an ultra-high throughput and accurate method for dual strand sequencing of DNA molecules.

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Title: Paired plus-minus sequencing is an ultra-high throughput and accurate method for dual strand sequencing of DNA molecules.
Authors: Cheng AP; École de Technologie Supérieure, Montréal, Québec, Canada.; Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Montréal, Québec, Canada.; New York Genome Center, New York, NY, United States., Rusinek I; Ultima Genomics, Fremont, California, United States., Sossin A; New York Genome Center, New York, NY, United States., Widman AJ; New York Genome Center, New York, NY, United States.; Memorial Sloan Kettering Cancer Center, New York, NY, United States., Meiri E; Ultima Genomics, Fremont, California, United States., Krieger G; Ultima Genomics, Fremont, California, United States., Hirschberg O; Ultima Genomics, Fremont, California, United States., Tov DS; Ultima Genomics, Fremont, California, United States., Gilad S; Ultima Genomics, Fremont, California, United States., Jaimovich A; Ultima Genomics, Fremont, California, United States., Barad O; Ultima Genomics, Fremont, California, United States., Avaylon S; New York Genome Center, New York, NY, United States., Rajagopalan S; New York Genome Center, New York, NY, United States.; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY, United States., Potenski C; New York Genome Center, New York, NY, United States.; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY, United States., Prieto T; New York Genome Center, New York, NY, United States.; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY, United States., Yuan DJ; New York Genome Center, New York, NY, United States.; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY, United States., Furatero R; New York Genome Center, New York, NY, United States., Runnels A; New York Genome Center, New York, NY, United States., Costa BM; Center for Human Genetics and Genomics, New York University Grossman School of Medicine, New York, NY, USA., Shoag JE; Department of Urology, University Hospitals Cleveland Medical Center, Case Western Reserve University School of Medicine, Cleveland, OH, USA., Assaad MA; Englander Institute for Precision Medicine, Weill Cornell Medicine, New York, NY, USA.; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY, USA., Sigouros M; Englander Institute for Precision Medicine, Weill Cornell Medicine, New York, NY, USA., Manohar J; Englander Institute for Precision Medicine, Weill Cornell Medicine, New York, NY, USA., King A; Englander Institute for Precision Medicine, Weill Cornell Medicine, New York, NY, USA., Wilkes D; Englander Institute for Precision Medicine, Weill Cornell Medicine, New York, NY, USA., Otilano J; Englander Institute for Precision Medicine, Weill Cornell Medicine, New York, NY, USA., Malbari MS; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY, United States., Elemento O; Englander Institute for Precision Medicine, Weill Cornell Medicine, New York, NY, USA.; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA., Mosquera JM; Englander Institute for Precision Medicine, Weill Cornell Medicine, New York, NY, USA.; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY, USA., Altorki NK; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY, United States., Saxena A; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY, United States., Callahan MK; Memorial Sloan Kettering Cancer Center, New York, NY, United States., Robine N; New York Genome Center, New York, NY, United States., Germer S; New York Genome Center, New York, NY, United States., Evrony GD; Center for Human Genetics and Genomics, New York University Grossman School of Medicine, New York, NY, USA.; Department of Pediatrics, New York University Grossman School of Medicine, New York, NY, USA.; Department of Neuroscience & Physiology, New York University Grossman School of Medicine, New York, NY, USA.; Institute for Systems Genetics, New York University Grossman School of Medicine, New York, NY, USA.; Perlmutter Cancer Center, New York University Langone Health, New York, NY, USA., Faltas BM; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY, United States.; Center for Human Genetics and Genomics, New York University Grossman School of Medicine, New York, NY, USA.; Department of Cell and Developmental Biology, Weill Cornell Medicine, New York, NY, USA., Landau DA; New York Genome Center, New York, NY, United States.; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY, United States.
Source: BioRxiv : the preprint server for biology [bioRxiv] 2025 Aug 14. Date of Electronic Publication: 2025 Aug 14.
Publication Type: Journal Article; Preprint
Journal Info: Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet ISSN: 2692-8205 (Electronic) Linking ISSN: 26928205 NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE
Database: MEDLINE Ultimate
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  Data: Paired plus-minus sequencing is an ultra-high throughput and accurate method for dual strand sequencing of DNA molecules.
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  Data: <searchLink fieldCode="AU" term="%22Cheng+AP%22">Cheng AP</searchLink>; École de Technologie Supérieure, Montréal, Québec, Canada.; Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Montréal, Québec, Canada.; New York Genome Center, New York, NY, United States.<br /><searchLink fieldCode="AU" term="%22Rusinek+I%22">Rusinek I</searchLink>; Ultima Genomics, Fremont, California, United States.<br /><searchLink fieldCode="AU" term="%22Sossin+A%22">Sossin A</searchLink>; New York Genome Center, New York, NY, United States.<br /><searchLink fieldCode="AU" term="%22Widman+AJ%22">Widman AJ</searchLink>; New York Genome Center, New York, NY, United States.; Memorial Sloan Kettering Cancer Center, New York, NY, United States.<br /><searchLink fieldCode="AU" term="%22Meiri+E%22">Meiri E</searchLink>; Ultima Genomics, Fremont, California, United States.<br /><searchLink fieldCode="AU" term="%22Krieger+G%22">Krieger G</searchLink>; Ultima Genomics, Fremont, California, United States.<br /><searchLink fieldCode="AU" term="%22Hirschberg+O%22">Hirschberg O</searchLink>; Ultima Genomics, Fremont, California, United States.<br /><searchLink fieldCode="AU" term="%22Tov+DS%22">Tov DS</searchLink>; Ultima Genomics, Fremont, California, United States.<br /><searchLink fieldCode="AU" term="%22Gilad+S%22">Gilad S</searchLink>; Ultima Genomics, Fremont, California, United States.<br /><searchLink fieldCode="AU" term="%22Jaimovich+A%22">Jaimovich A</searchLink>; Ultima Genomics, Fremont, California, United States.<br /><searchLink fieldCode="AU" term="%22Barad+O%22">Barad O</searchLink>; Ultima Genomics, Fremont, California, United States.<br /><searchLink fieldCode="AU" term="%22Avaylon+S%22">Avaylon S</searchLink>; New York Genome Center, New York, NY, United States.<br /><searchLink fieldCode="AU" term="%22Rajagopalan+S%22">Rajagopalan S</searchLink>; New York Genome Center, New York, NY, United States.; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY, United States.<br /><searchLink fieldCode="AU" term="%22Potenski+C%22">Potenski C</searchLink>; New York Genome Center, New York, NY, United States.; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY, United States.<br /><searchLink fieldCode="AU" term="%22Prieto+T%22">Prieto T</searchLink>; New York Genome Center, New York, NY, United States.; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY, United States.<br /><searchLink fieldCode="AU" term="%22Yuan+DJ%22">Yuan DJ</searchLink>; New York Genome Center, New York, NY, United States.; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY, United States.<br /><searchLink fieldCode="AU" term="%22Furatero+R%22">Furatero R</searchLink>; New York Genome Center, New York, NY, United States.<br /><searchLink fieldCode="AU" term="%22Runnels+A%22">Runnels A</searchLink>; New York Genome Center, New York, NY, United States.<br /><searchLink fieldCode="AU" term="%22Costa+BM%22">Costa BM</searchLink>; Center for Human Genetics and Genomics, New York University Grossman School of Medicine, New York, NY, USA.<br /><searchLink fieldCode="AU" term="%22Shoag+JE%22">Shoag JE</searchLink>; Department of Urology, University Hospitals Cleveland Medical Center, Case Western Reserve University School of Medicine, Cleveland, OH, USA.<br /><searchLink fieldCode="AU" term="%22Assaad+MA%22">Assaad MA</searchLink>; Englander Institute for Precision Medicine, Weill Cornell Medicine, New York, NY, USA.; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY, USA.<br /><searchLink fieldCode="AU" term="%22Sigouros+M%22">Sigouros M</searchLink>; Englander Institute for Precision Medicine, Weill Cornell Medicine, New York, NY, USA.<br /><searchLink fieldCode="AU" term="%22Manohar+J%22">Manohar J</searchLink>; Englander Institute for Precision Medicine, Weill Cornell Medicine, New York, NY, USA.<br /><searchLink fieldCode="AU" term="%22King+A%22">King A</searchLink>; Englander Institute for Precision Medicine, Weill Cornell Medicine, New York, NY, USA.<br /><searchLink fieldCode="AU" term="%22Wilkes+D%22">Wilkes D</searchLink>; Englander Institute for Precision Medicine, Weill Cornell Medicine, New York, NY, USA.<br /><searchLink fieldCode="AU" term="%22Otilano+J%22">Otilano J</searchLink>; Englander Institute for Precision Medicine, Weill Cornell Medicine, New York, NY, USA.<br /><searchLink fieldCode="AU" term="%22Malbari+MS%22">Malbari MS</searchLink>; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY, United States.<br /><searchLink fieldCode="AU" term="%22Elemento+O%22">Elemento O</searchLink>; Englander Institute for Precision Medicine, Weill Cornell Medicine, New York, NY, USA.; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA.<br /><searchLink fieldCode="AU" term="%22Mosquera+JM%22">Mosquera JM</searchLink>; Englander Institute for Precision Medicine, Weill Cornell Medicine, New York, NY, USA.; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY, USA.<br /><searchLink fieldCode="AU" term="%22Altorki+NK%22">Altorki NK</searchLink>; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY, United States.<br /><searchLink fieldCode="AU" term="%22Saxena+A%22">Saxena A</searchLink>; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY, United States.<br /><searchLink fieldCode="AU" term="%22Callahan+MK%22">Callahan MK</searchLink>; Memorial Sloan Kettering Cancer Center, New York, NY, United States.<br /><searchLink fieldCode="AU" term="%22Robine+N%22">Robine N</searchLink>; New York Genome Center, New York, NY, United States.<br /><searchLink fieldCode="AU" term="%22Germer+S%22">Germer S</searchLink>; New York Genome Center, New York, NY, United States.<br /><searchLink fieldCode="AU" term="%22Evrony+GD%22">Evrony GD</searchLink>; Center for Human Genetics and Genomics, New York University Grossman School of Medicine, New York, NY, USA.; Department of Pediatrics, New York University Grossman School of Medicine, New York, NY, USA.; Department of Neuroscience & Physiology, New York University Grossman School of Medicine, New York, NY, USA.; Institute for Systems Genetics, New York University Grossman School of Medicine, New York, NY, USA.; Perlmutter Cancer Center, New York University Langone Health, New York, NY, USA.<br /><searchLink fieldCode="AU" term="%22Faltas+BM%22">Faltas BM</searchLink>; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY, United States.; Center for Human Genetics and Genomics, New York University Grossman School of Medicine, New York, NY, USA.; Department of Cell and Developmental Biology, Weill Cornell Medicine, New York, NY, USA.<br /><searchLink fieldCode="AU" term="%22Landau+DA%22">Landau DA</searchLink>; New York Genome Center, New York, NY, United States.; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY, United States.
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