Scalable extracellular expression of tag-free MPT64 protein in E. coli via pelB signal optimization: a step toward tuberculosis diagnostic antigen preparation.
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| Title: | Scalable extracellular expression of tag-free MPT64 protein in E. coli via pelB signal optimization: a step toward tuberculosis diagnostic antigen preparation. |
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| Authors: | Kusuma SAF; Department of Biology Pharmacy, Faculty of Pharmacy, Padjadjaran University, Sumedang, West Java, Indonesia., Fatinah Y; Department of Biology Pharmacy, Faculty of Pharmacy, Padjadjaran University, Sumedang, West Java, Indonesia., Fadhlillah M; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Padjadjaran University, Indonesia.; Research Center of Molecular Biotechnology and Bioinformatics, Padjadjaran University, Bandung, Indonesia., Parwati I; Clinical Pathology Department, Faculty of Medical, Padjadjaran University, Indonesia., Rukayadi Y; Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, Malaysia., Subroto T; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Padjadjaran University, Indonesia.; Research Center of Molecular Biotechnology and Bioinformatics, Padjadjaran University, Bandung, Indonesia. |
| Source: | Preparative biochemistry & biotechnology [Prep Biochem Biotechnol] 2026; Vol. 56 (3), pp. 483-493. Date of Electronic Publication: 2025 Aug 25. |
| Publication Type: | Journal Article |
| Journal Info: | Publisher: Taylor & Francis Country of Publication: England NLM ID: 9607037 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2297 (Electronic) Linking ISSN: 10826068 NLM ISO Abbreviation: Prep Biochem Biotechnol Subsets: MEDLINE |
| Database: | MEDLINE Ultimate |
| FullText | Text: Availability: 0 |
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| Header | DbId: mdl DbLabel: MEDLINE Ultimate An: 40852953 AccessLevel: 2 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Scalable extracellular expression of tag-free MPT64 protein in E. coli via pelB signal optimization: a step toward tuberculosis diagnostic antigen preparation. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AU" term="%22Kusuma+SAF%22">Kusuma SAF</searchLink>; Department of Biology Pharmacy, Faculty of Pharmacy, Padjadjaran University, Sumedang, West Java, Indonesia.<br /><searchLink fieldCode="AU" term="%22Fatinah+Y%22">Fatinah Y</searchLink>; Department of Biology Pharmacy, Faculty of Pharmacy, Padjadjaran University, Sumedang, West Java, Indonesia.<br /><searchLink fieldCode="AU" term="%22Fadhlillah+M%22">Fadhlillah M</searchLink>; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Padjadjaran University, Indonesia.; Research Center of Molecular Biotechnology and Bioinformatics, Padjadjaran University, Bandung, Indonesia.<br /><searchLink fieldCode="AU" term="%22Parwati+I%22">Parwati I</searchLink>; Clinical Pathology Department, Faculty of Medical, Padjadjaran University, Indonesia.<br /><searchLink fieldCode="AU" term="%22Rukayadi+Y%22">Rukayadi Y</searchLink>; Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, Malaysia.<br /><searchLink fieldCode="AU" term="%22Subroto+T%22">Subroto T</searchLink>; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Padjadjaran University, Indonesia.; Research Center of Molecular Biotechnology and Bioinformatics, Padjadjaran University, Bandung, Indonesia. – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%229607037%22">Preparative biochemistry & biotechnology</searchLink> [Prep Biochem Biotechnol] 2026; Vol. 56 (3), pp. 483-493. <i>Date of Electronic Publication: </i>2025 Aug 25. – Name: TypePub Label: Publication Type Group: TypPub Data: Journal Article – Name: TitleSource Label: Journal Info Group: Src Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Taylor+%26+Francis%22">Taylor & Francis </searchLink><i>Country of Publication: </i>England <i>NLM ID: </i>9607037 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1532-2297 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2210826068%22">10826068 </searchLink><i>NLM ISO Abbreviation: </i>Prep Biochem Biotechnol <i>Subsets: </i>MEDLINE |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=mdl&AN=40852953 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1080/10826068.2025.2551369 Languages: – Code: eng Text: English PhysicalDescription: Pagination: StartPage: 483 Titles: – TitleFull: Scalable extracellular expression of tag-free MPT64 protein in E. coli via pelB signal optimization: a step toward tuberculosis diagnostic antigen preparation. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Kusuma SAF – PersonEntity: Name: NameFull: Fatinah Y – PersonEntity: Name: NameFull: Fadhlillah M – PersonEntity: Name: NameFull: Parwati I – PersonEntity: Name: NameFull: Rukayadi Y – PersonEntity: Name: NameFull: Subroto T IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Text: 2026 Type: published Y: 2026 Identifiers: – Type: issn-electronic Value: 1532-2297 Numbering: – Type: volume Value: 56 – Type: issue Value: 3 Titles: – TitleFull: Preparative biochemistry & biotechnology Type: main |
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