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Flow cytometry protocol for cell death analysis in glioblastoma organoids: A technical note.

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Title: Flow cytometry protocol for cell death analysis in glioblastoma organoids: A technical note.
Authors: Potthoff AL; Department of Neurosurgery, University Hospital Bonn, Bonn, Germany.; Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany.; Institute of Neuropathology, University Hospital Bonn, Bonn, Germany., Hsieh MC; Department of Neurosurgery, University Hospital Bonn, Bonn, Germany.; Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany., Melhem A; Department of Neurosurgery, University Hospital Bonn, Bonn, Germany.; Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany., Ng SS; Institute of Experimental Oncology, University Hospital Bonn, Bonn, Germany., Pregler BEF; Department of Neurosurgery, University Hospital Bonn, Bonn, Germany.; Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany., Vieregge A; Department of Neurosurgery, University Hospital Bonn, Bonn, Germany.; Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany., Raspe M; Department of Neurosurgery, University Hospital Bonn, Bonn, Germany.; Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany., Friker LL; Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany.; Institute of Neuropathology, University Hospital Bonn, Bonn, Germany.; Institute of Experimental Oncology, University Hospital Bonn, Bonn, Germany., Zeyen T; Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany.; Department of Neurooncology, Center for Neurology, University Hospital Bonn, Bonn, Germany., Layer JP; Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany.; Institute of Experimental Oncology, University Hospital Bonn, Bonn, Germany.; Department of Radiation Oncology, University Hospital Bonn, Bonn, Germany., Dolf A; Flow Cytometry Core Facility, Medical Faculty, University of Bonn, Bonn, Germany., Toma MI; Department of Pathology, University Hospital Bonn, Bonn, Germany., Waha A; Institute of Neuropathology, University Hospital Bonn, Bonn, Germany., Pietsch T; Institute of Neuropathology, University Hospital Bonn, Bonn, Germany., Westhoff MA; Department of Pediatrics and Adolescent Medicine, University Medical Center Ulm, Ulm, Germany., Vatter H; Department of Neurosurgery, University Hospital Bonn, Bonn, Germany.; Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany., Hölzel M; Institute of Experimental Oncology, University Hospital Bonn, Bonn, Germany., Herrlinger U; Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany.; Department of Neurooncology, Center for Neurology, University Hospital Bonn, Bonn, Germany., Schneider M; Department of Neurosurgery, University Hospital Bonn, Bonn, Germany.; Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany.
Source: PloS one [PLoS One] 2025 Sep 23; Vol. 20 (9), pp. e0327660. Date of Electronic Publication: 2025 Sep 23 (Print Publication: 2025).
Publication Type: Journal Article
Journal Info: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: Flow cytometry protocol for cell death analysis in glioblastoma organoids: A technical note.
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  Data: <searchLink fieldCode="AU" term="%22Potthoff+AL%22">Potthoff AL</searchLink>; Department of Neurosurgery, University Hospital Bonn, Bonn, Germany.; Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany.; Institute of Neuropathology, University Hospital Bonn, Bonn, Germany.<br /><searchLink fieldCode="AU" term="%22Hsieh+MC%22">Hsieh MC</searchLink>; Department of Neurosurgery, University Hospital Bonn, Bonn, Germany.; Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany.<br /><searchLink fieldCode="AU" term="%22Melhem+A%22">Melhem A</searchLink>; Department of Neurosurgery, University Hospital Bonn, Bonn, Germany.; Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany.<br /><searchLink fieldCode="AU" term="%22Ng+SS%22">Ng SS</searchLink>; Institute of Experimental Oncology, University Hospital Bonn, Bonn, Germany.<br /><searchLink fieldCode="AU" term="%22Pregler+BEF%22">Pregler BEF</searchLink>; Department of Neurosurgery, University Hospital Bonn, Bonn, Germany.; Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany.<br /><searchLink fieldCode="AU" term="%22Vieregge+A%22">Vieregge A</searchLink>; Department of Neurosurgery, University Hospital Bonn, Bonn, Germany.; Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany.<br /><searchLink fieldCode="AU" term="%22Raspe+M%22">Raspe M</searchLink>; Department of Neurosurgery, University Hospital Bonn, Bonn, Germany.; Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany.<br /><searchLink fieldCode="AU" term="%22Friker+LL%22">Friker LL</searchLink>; Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany.; Institute of Neuropathology, University Hospital Bonn, Bonn, Germany.; Institute of Experimental Oncology, University Hospital Bonn, Bonn, Germany.<br /><searchLink fieldCode="AU" term="%22Zeyen+T%22">Zeyen T</searchLink>; Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany.; Department of Neurooncology, Center for Neurology, University Hospital Bonn, Bonn, Germany.<br /><searchLink fieldCode="AU" term="%22Layer+JP%22">Layer JP</searchLink>; Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany.; Institute of Experimental Oncology, University Hospital Bonn, Bonn, Germany.; Department of Radiation Oncology, University Hospital Bonn, Bonn, Germany.<br /><searchLink fieldCode="AU" term="%22Dolf+A%22">Dolf A</searchLink>; Flow Cytometry Core Facility, Medical Faculty, University of Bonn, Bonn, Germany.<br /><searchLink fieldCode="AU" term="%22Toma+MI%22">Toma MI</searchLink>; Department of Pathology, University Hospital Bonn, Bonn, Germany.<br /><searchLink fieldCode="AU" term="%22Waha+A%22">Waha A</searchLink>; Institute of Neuropathology, University Hospital Bonn, Bonn, Germany.<br /><searchLink fieldCode="AU" term="%22Pietsch+T%22">Pietsch T</searchLink>; Institute of Neuropathology, University Hospital Bonn, Bonn, Germany.<br /><searchLink fieldCode="AU" term="%22Westhoff+MA%22">Westhoff MA</searchLink>; Department of Pediatrics and Adolescent Medicine, University Medical Center Ulm, Ulm, Germany.<br /><searchLink fieldCode="AU" term="%22Vatter+H%22">Vatter H</searchLink>; Department of Neurosurgery, University Hospital Bonn, Bonn, Germany.; Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany.<br /><searchLink fieldCode="AU" term="%22Hölzel+M%22">Hölzel M</searchLink>; Institute of Experimental Oncology, University Hospital Bonn, Bonn, Germany.<br /><searchLink fieldCode="AU" term="%22Herrlinger+U%22">Herrlinger U</searchLink>; Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany.; Department of Neurooncology, Center for Neurology, University Hospital Bonn, Bonn, Germany.<br /><searchLink fieldCode="AU" term="%22Schneider+M%22">Schneider M</searchLink>; Department of Neurosurgery, University Hospital Bonn, Bonn, Germany.; Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany.
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