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Comparative analysis of RT-qPCR assay sensitivity and process limit of detection for Japanese encephalitis virus (JEV) detection in piggery wastewater.

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Title: Comparative analysis of RT-qPCR assay sensitivity and process limit of detection for Japanese encephalitis virus (JEV) detection in piggery wastewater.
Authors: Freddi A; CSIRO Environment, Ecosciences Precinct, 41 Boggo Road, Dutton Park, QLD 4102, Australia; Department of Biomolecular Sciences, University of Urbino, Urbino 61029, Italy; Institute of Marine Biological Resources and Biotechnologies - CNR IRBIM, Ancona 60125, Italy., Gebrewold M; CSIRO Environment, Ecosciences Precinct, 41 Boggo Road, Dutton Park, QLD 4102, Australia., Smith WJM; CSIRO Environment, Ecosciences Precinct, 41 Boggo Road, Dutton Park, QLD 4102, Australia., Manini E; Institute of Marine Biological Resources and Biotechnologies - CNR IRBIM, Ancona 60125, Italy., Simpson SL; CSIRO Environment, Ecosciences Precinct, 41 Boggo Road, Dutton Park, QLD 4102, Australia., Ahmed W; CSIRO Environment, Ecosciences Precinct, 41 Boggo Road, Dutton Park, QLD 4102, Australia. Electronic address: Warish.Ahmed@csiro.au.
Source: Journal of virological methods [J Virol Methods] 2026 Feb; Vol. 340, pp. 115272. Date of Electronic Publication: 2025 Oct 01.
Publication Type: Journal Article; Comparative Study; Evaluation Study
Journal Info: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE
Database: MEDLINE Ultimate
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PubType: Academic Journal
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  Data: Comparative analysis of RT-qPCR assay sensitivity and process limit of detection for Japanese encephalitis virus (JEV) detection in piggery wastewater.
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  Data: <searchLink fieldCode="AU" term="%22Freddi+A%22">Freddi A</searchLink>; CSIRO Environment, Ecosciences Precinct, 41 Boggo Road, Dutton Park, QLD 4102, Australia; Department of Biomolecular Sciences, University of Urbino, Urbino 61029, Italy; Institute of Marine Biological Resources and Biotechnologies - CNR IRBIM, Ancona 60125, Italy.<br /><searchLink fieldCode="AU" term="%22Gebrewold+M%22">Gebrewold M</searchLink>; CSIRO Environment, Ecosciences Precinct, 41 Boggo Road, Dutton Park, QLD 4102, Australia.<br /><searchLink fieldCode="AU" term="%22Smith+WJM%22">Smith WJM</searchLink>; CSIRO Environment, Ecosciences Precinct, 41 Boggo Road, Dutton Park, QLD 4102, Australia.<br /><searchLink fieldCode="AU" term="%22Manini+E%22">Manini E</searchLink>; Institute of Marine Biological Resources and Biotechnologies - CNR IRBIM, Ancona 60125, Italy.<br /><searchLink fieldCode="AU" term="%22Simpson+SL%22">Simpson SL</searchLink>; CSIRO Environment, Ecosciences Precinct, 41 Boggo Road, Dutton Park, QLD 4102, Australia.<br /><searchLink fieldCode="AU" term="%22Ahmed+W%22">Ahmed W</searchLink>; CSIRO Environment, Ecosciences Precinct, 41 Boggo Road, Dutton Park, QLD 4102, Australia. Electronic address: Warish.Ahmed@csiro.au.
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  Data: <searchLink fieldCode="JN" term="%228005839%22">Journal of virological methods</searchLink> [J Virol Methods] 2026 Feb; Vol. 340, pp. 115272. <i>Date of Electronic Publication: </i>2025 Oct 01.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Elsevier%2FNorth-Holland+Biomedical+Press%22">Elsevier/North-Holland Biomedical Press </searchLink><i>Country of Publication: </i>Netherlands <i>NLM ID: </i>8005839 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1879-0984 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2201660934%22">01660934 </searchLink><i>NLM ISO Abbreviation: </i>J Virol Methods <i>Subsets: </i>MEDLINE
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        Value: 10.1016/j.jviromet.2025.115272
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      – Code: eng
        Text: English
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        StartPage: 115272
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      – TitleFull: Comparative analysis of RT-qPCR assay sensitivity and process limit of detection for Japanese encephalitis virus (JEV) detection in piggery wastewater.
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            NameFull: Freddi A
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              Text: 2026 Feb
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              Y: 2026
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              Value: 340
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