Development of a real-time polymerase chain reaction (RT-PCR) assay for simultaneous detection of Helicobacter pylori infection and genetic mutations associated with clarithromycin resistance: A single-center, cross-sectional study from Singapore.

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Title: Development of a real-time polymerase chain reaction (RT-PCR) assay for simultaneous detection of Helicobacter pylori infection and genetic mutations associated with clarithromycin resistance: A single-center, cross-sectional study from Singapore.
Authors: Jiang B; Department of Laboratory Medicine, Changi General Hospital, 2 Simei Street 3, Singapore 529889, Singapore. Electronic address: jiang_boran@cgh.com.sg., Koo SH; Research Laboratory, Changi General Hospital, 2 Simei Street 3, Singapore 529889, Singapore. Electronic address: seok_hwee_koo@cgh.com.sg., Ang DSW; Department of Gastroenterology and Hepatology, Changi General Hospital, 2 Simei Street 3, Singapore 529889, Singapore. Electronic address: daphne.ang.s.w@singhealth.com.sg., Ang TL; Department of Gastroenterology and Hepatology, Changi General Hospital, 2 Simei Street 3, Singapore 529889, Singapore. Electronic address: ang.tiing.leong@singhealth.com.sg., Lee HZ; Department of Laboratory Medicine, Changi General Hospital, 2 Simei Street 3, Singapore 529889, Singapore. Electronic address: Hui_Zi_Lee@cgh.com.sg., Tan TY; Department of Laboratory Medicine, Changi General Hospital, 2 Simei Street 3, Singapore 529889, Singapore. Electronic address: tan.thean.yen@singhealth.com.sg.
Source: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2026 Feb; Vol. 114 (2), pp. 117131. Date of Electronic Publication: 2025 Sep 28.
Publication Type: Journal Article
Journal Info: Publisher: Elsevier Biomedical Country of Publication: United States NLM ID: 8305899 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0070 (Electronic) Linking ISSN: 07328893 NLM ISO Abbreviation: Diagn Microbiol Infect Dis Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:1879-0070
DOI:10.1016/j.diagmicrobio.2025.117131