Development of a real-time polymerase chain reaction (RT-PCR) assay for simultaneous detection of Helicobacter pylori infection and genetic mutations associated with clarithromycin resistance: A single-center, cross-sectional study from Singapore.

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Title: Development of a real-time polymerase chain reaction (RT-PCR) assay for simultaneous detection of Helicobacter pylori infection and genetic mutations associated with clarithromycin resistance: A single-center, cross-sectional study from Singapore.
Authors: Jiang B; Department of Laboratory Medicine, Changi General Hospital, 2 Simei Street 3, Singapore 529889, Singapore. Electronic address: jiang_boran@cgh.com.sg., Koo SH; Research Laboratory, Changi General Hospital, 2 Simei Street 3, Singapore 529889, Singapore. Electronic address: seok_hwee_koo@cgh.com.sg., Ang DSW; Department of Gastroenterology and Hepatology, Changi General Hospital, 2 Simei Street 3, Singapore 529889, Singapore. Electronic address: daphne.ang.s.w@singhealth.com.sg., Ang TL; Department of Gastroenterology and Hepatology, Changi General Hospital, 2 Simei Street 3, Singapore 529889, Singapore. Electronic address: ang.tiing.leong@singhealth.com.sg., Lee HZ; Department of Laboratory Medicine, Changi General Hospital, 2 Simei Street 3, Singapore 529889, Singapore. Electronic address: Hui_Zi_Lee@cgh.com.sg., Tan TY; Department of Laboratory Medicine, Changi General Hospital, 2 Simei Street 3, Singapore 529889, Singapore. Electronic address: tan.thean.yen@singhealth.com.sg.
Source: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2026 Feb; Vol. 114 (2), pp. 117131. Date of Electronic Publication: 2025 Sep 28.
Publication Type: Journal Article
Journal Info: Publisher: Elsevier Biomedical Country of Publication: United States NLM ID: 8305899 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0070 (Electronic) Linking ISSN: 07328893 NLM ISO Abbreviation: Diagn Microbiol Infect Dis Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: Development of a real-time polymerase chain reaction (RT-PCR) assay for simultaneous detection of Helicobacter pylori infection and genetic mutations associated with clarithromycin resistance: A single-center, cross-sectional study from Singapore.
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  Data: <searchLink fieldCode="AU" term="%22Jiang+B%22">Jiang B</searchLink>; Department of Laboratory Medicine, Changi General Hospital, 2 Simei Street 3, Singapore 529889, Singapore. Electronic address: jiang_boran@cgh.com.sg.<br /><searchLink fieldCode="AU" term="%22Koo+SH%22">Koo SH</searchLink>; Research Laboratory, Changi General Hospital, 2 Simei Street 3, Singapore 529889, Singapore. Electronic address: seok_hwee_koo@cgh.com.sg.<br /><searchLink fieldCode="AU" term="%22Ang+DSW%22">Ang DSW</searchLink>; Department of Gastroenterology and Hepatology, Changi General Hospital, 2 Simei Street 3, Singapore 529889, Singapore. Electronic address: daphne.ang.s.w@singhealth.com.sg.<br /><searchLink fieldCode="AU" term="%22Ang+TL%22">Ang TL</searchLink>; Department of Gastroenterology and Hepatology, Changi General Hospital, 2 Simei Street 3, Singapore 529889, Singapore. Electronic address: ang.tiing.leong@singhealth.com.sg.<br /><searchLink fieldCode="AU" term="%22Lee+HZ%22">Lee HZ</searchLink>; Department of Laboratory Medicine, Changi General Hospital, 2 Simei Street 3, Singapore 529889, Singapore. Electronic address: Hui_Zi_Lee@cgh.com.sg.<br /><searchLink fieldCode="AU" term="%22Tan+TY%22">Tan TY</searchLink>; Department of Laboratory Medicine, Changi General Hospital, 2 Simei Street 3, Singapore 529889, Singapore. Electronic address: tan.thean.yen@singhealth.com.sg.
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  Data: <searchLink fieldCode="JN" term="%228305899%22">Diagnostic microbiology and infectious disease</searchLink> [Diagn Microbiol Infect Dis] 2026 Feb; Vol. 114 (2), pp. 117131. <i>Date of Electronic Publication: </i>2025 Sep 28.
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        Value: 10.1016/j.diagmicrobio.2025.117131
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      – TitleFull: Development of a real-time polymerase chain reaction (RT-PCR) assay for simultaneous detection of Helicobacter pylori infection and genetic mutations associated with clarithromycin resistance: A single-center, cross-sectional study from Singapore.
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            NameFull: Koo SH
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              Text: 2026 Feb
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