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Wet Lab Protocols Matter: Choice of DNA Extraction and Library Preparation Protocols Bias Ancient Oral Microbiome Recovery.

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Title: Wet Lab Protocols Matter: Choice of DNA Extraction and Library Preparation Protocols Bias Ancient Oral Microbiome Recovery.
Authors: Wright SL; Department of Anthropology, The Pennsylvania State University, University Park, Pennsylvania, USA.; One Health Microbiome Center, The Pennsylvania State University, University Park, Pennsylvania, USA.; College of Health Solutions, Arizona State University, Phoenix, Arizona, USA.; Center for Health Through Microbiomes, The Biodesign Institute, Arizona State University, Tempe, Arizona, USA., Abdul-Aziz M; Australian Centre for Ancient DNA, School of Biological Sciences and the Environment Institute, and Centre of Excellence for Australian Biodiversity and Heritage, The University of Adelaide, Adelaide, Australia., Blaha GN; Department of Anthropology, The Pennsylvania State University, University Park, Pennsylvania, USA., Ta CK; Department of Anthropology, The Pennsylvania State University, University Park, Pennsylvania, USA.; One Health Microbiome Center, The Pennsylvania State University, University Park, Pennsylvania, USA., Gancz A; Department of Anthropology, The Pennsylvania State University, University Park, Pennsylvania, USA.; One Health Microbiome Center, The Pennsylvania State University, University Park, Pennsylvania, USA., Ademola-Popoola IJ; Department of Anthropology, The Pennsylvania State University, University Park, Pennsylvania, USA.; One Health Microbiome Center, The Pennsylvania State University, University Park, Pennsylvania, USA., Szécsényi-Nagy A; ELTE Research Centre for the Humanities, Institute of Archaeogenomics, MTA-BTK Lendület 'Momentum' Research Group, Budapest, Hungary., Sereno PC; Department of Organismal Biology and Anatomy and Committee on Evolutionary Biology, University of Chicago, Chicago, Illinois, USA.; Institut de Recherches en Sciences Humains, Université Abdou Moumouni de Niamey, Niamey, Niger République., Weyrich LS; Department of Anthropology, The Pennsylvania State University, University Park, Pennsylvania, USA.; One Health Microbiome Center, The Pennsylvania State University, University Park, Pennsylvania, USA.; Australian Centre for Ancient DNA, School of Biological Sciences and the Environment Institute, and Centre of Excellence for Australian Biodiversity and Heritage, The University of Adelaide, Adelaide, Australia.
Source: Molecular ecology resources [Mol Ecol Resour] 2025 Nov; Vol. 25 (8), pp. e70054. Date of Electronic Publication: 2025 Oct 06.
Publication Type: Journal Article
Journal Info: Publisher: Blackwell Country of Publication: England NLM ID: 101465604 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1755-0998 (Electronic) Linking ISSN: 1755098X NLM ISO Abbreviation: Mol Ecol Resour Subsets: MEDLINE
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  Data: Wet Lab Protocols Matter: Choice of DNA Extraction and Library Preparation Protocols Bias Ancient Oral Microbiome Recovery.
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  Data: <searchLink fieldCode="AU" term="%22Wright+SL%22">Wright SL</searchLink>; Department of Anthropology, The Pennsylvania State University, University Park, Pennsylvania, USA.; One Health Microbiome Center, The Pennsylvania State University, University Park, Pennsylvania, USA.; College of Health Solutions, Arizona State University, Phoenix, Arizona, USA.; Center for Health Through Microbiomes, The Biodesign Institute, Arizona State University, Tempe, Arizona, USA.<br /><searchLink fieldCode="AU" term="%22Abdul-Aziz+M%22">Abdul-Aziz M</searchLink>; Australian Centre for Ancient DNA, School of Biological Sciences and the Environment Institute, and Centre of Excellence for Australian Biodiversity and Heritage, The University of Adelaide, Adelaide, Australia.<br /><searchLink fieldCode="AU" term="%22Blaha+GN%22">Blaha GN</searchLink>; Department of Anthropology, The Pennsylvania State University, University Park, Pennsylvania, USA.<br /><searchLink fieldCode="AU" term="%22Ta+CK%22">Ta CK</searchLink>; Department of Anthropology, The Pennsylvania State University, University Park, Pennsylvania, USA.; One Health Microbiome Center, The Pennsylvania State University, University Park, Pennsylvania, USA.<br /><searchLink fieldCode="AU" term="%22Gancz+A%22">Gancz A</searchLink>; Department of Anthropology, The Pennsylvania State University, University Park, Pennsylvania, USA.; One Health Microbiome Center, The Pennsylvania State University, University Park, Pennsylvania, USA.<br /><searchLink fieldCode="AU" term="%22Ademola-Popoola+IJ%22">Ademola-Popoola IJ</searchLink>; Department of Anthropology, The Pennsylvania State University, University Park, Pennsylvania, USA.; One Health Microbiome Center, The Pennsylvania State University, University Park, Pennsylvania, USA.<br /><searchLink fieldCode="AU" term="%22Szécsényi-Nagy+A%22">Szécsényi-Nagy A</searchLink>; ELTE Research Centre for the Humanities, Institute of Archaeogenomics, MTA-BTK Lendület 'Momentum' Research Group, Budapest, Hungary.<br /><searchLink fieldCode="AU" term="%22Sereno+PC%22">Sereno PC</searchLink>; Department of Organismal Biology and Anatomy and Committee on Evolutionary Biology, University of Chicago, Chicago, Illinois, USA.; Institut de Recherches en Sciences Humains, Université Abdou Moumouni de Niamey, Niamey, Niger République.<br /><searchLink fieldCode="AU" term="%22Weyrich+LS%22">Weyrich LS</searchLink>; Department of Anthropology, The Pennsylvania State University, University Park, Pennsylvania, USA.; One Health Microbiome Center, The Pennsylvania State University, University Park, Pennsylvania, USA.; Australian Centre for Ancient DNA, School of Biological Sciences and the Environment Institute, and Centre of Excellence for Australian Biodiversity and Heritage, The University of Adelaide, Adelaide, Australia.
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  Data: <searchLink fieldCode="JN" term="%22101465604%22">Molecular ecology resources</searchLink> [Mol Ecol Resour] 2025 Nov; Vol. 25 (8), pp. e70054. <i>Date of Electronic Publication: </i>2025 Oct 06.
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              Text: 2025 Nov
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