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Novel Type IIS-Based Library Assembly Technique for Developing Nanobodies Targeting IPNv VP2 Protein.

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Title: Novel Type IIS-Based Library Assembly Technique for Developing Nanobodies Targeting IPNv VP2 Protein.
Authors: Pino-Belmar C; Institute of Biochemistry and Microbiology, Faculty of Sciences, Universidad Austral de Chile, Valdivia 5110566, Chile., Himelreichs J; Institute of Medicine, Faculty of Medicine, Universidad Austral de Chile, Valdivia 5110566, Chile., Deride C; Institute of Medicine, Faculty of Medicine, Universidad Austral de Chile, Valdivia 5110566, Chile., Matute T; Agencia Nacional de Investigación y Desarrollo-Millennium Science Initiative Program-Millennium Institute for Integrative Biology (iBio), Schools of Engineering, Medicine and Biological Sciences, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile.; Institute for Biological and Medical Engineering, Schools of Engineering, Medicine and Biological Sciences, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile., Nuñez I; Agencia Nacional de Investigación y Desarrollo-Millennium Science Initiative Program-Millennium Institute for Integrative Biology (iBio), Schools of Engineering, Medicine and Biological Sciences, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile.; Institute for Biological and Medical Engineering, Schools of Engineering, Medicine and Biological Sciences, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile., Cazaux S; Agencia Nacional de Investigación y Desarrollo-Millennium Science Initiative Program-Millennium Institute for Integrative Biology (iBio), Schools of Engineering, Medicine and Biological Sciences, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile.; Institute for Biological and Medical Engineering, Schools of Engineering, Medicine and Biological Sciences, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile., Federici F; Agencia Nacional de Investigación y Desarrollo-Millennium Science Initiative Program-Millennium Institute for Integrative Biology (iBio), Schools of Engineering, Medicine and Biological Sciences, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile.; Institute for Biological and Medical Engineering, Schools of Engineering, Medicine and Biological Sciences, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile., Moreno-Mendieta K; Institute of Biochemistry and Microbiology, Faculty of Sciences, Universidad Austral de Chile, Valdivia 5110566, Chile., Soto-Rauch G; Institute of Biochemistry and Microbiology, Faculty of Sciences, Universidad Austral de Chile, Valdivia 5110566, Chile., Castro J; Bain & Company, Santiago 7550268, Chile., Frenkel V; Bain & Company, Santiago 7550268, Chile., Ho JH; Bain & Company, Santiago 7550268, Chile., Ascencios D; Bain & Company, Santiago 7550268, Chile., Sanhueza Teneo D; Institute of Immunology and Parasitology, Faculty of Medicine, Universidad Austral de Chile, Valdivia 5110566, Chile., Munizaga J; Biochemistry School, Faculty of Sciences, Universidad Austral de Chile, Valdivia 5110566, Chile.; Facultad de Ciencias para el Cuidado de la Salud, Universidad San Sebastián, Valdivia 5090000, Chile., Haussmann D; Departamento de Ciencias Básicas, Facultad de Ciencias, Universidad Santo Tomás, Valdivia 5110566, Chile., Rojas-Fernandez A; Institute of Medicine, Faculty of Medicine, Universidad Austral de Chile, Valdivia 5110566, Chile.; Berking Biotechnology, Valdivia 5090000, Chile., Valverde JF; Institute of Biochemistry and Microbiology, Faculty of Sciences, Universidad Austral de Chile, Valdivia 5110566, Chile.; Interdisciplinary Center for Aquaculture Research (INCAR), Concepción 4030000, Chile., Valenzuela-Nieto G; Facultad de Ciencias para el Cuidado de la Salud, Universidad San Sebastián, Valdivia 5090000, Chile.
Source: International journal of molecular sciences [Int J Mol Sci] 2025 Sep 25; Vol. 26 (19). Date of Electronic Publication: 2025 Sep 25.
Publication Type: Journal Article
Journal Info: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
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  Data: <searchLink fieldCode="AU" term="%22Pino-Belmar+C%22">Pino-Belmar C</searchLink>; Institute of Biochemistry and Microbiology, Faculty of Sciences, Universidad Austral de Chile, Valdivia 5110566, Chile.<br /><searchLink fieldCode="AU" term="%22Himelreichs+J%22">Himelreichs J</searchLink>; Institute of Medicine, Faculty of Medicine, Universidad Austral de Chile, Valdivia 5110566, Chile.<br /><searchLink fieldCode="AU" term="%22Deride+C%22">Deride C</searchLink>; Institute of Medicine, Faculty of Medicine, Universidad Austral de Chile, Valdivia 5110566, Chile.<br /><searchLink fieldCode="AU" term="%22Matute+T%22">Matute T</searchLink>; Agencia Nacional de Investigación y Desarrollo-Millennium Science Initiative Program-Millennium Institute for Integrative Biology (iBio), Schools of Engineering, Medicine and Biological Sciences, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile.; Institute for Biological and Medical Engineering, Schools of Engineering, Medicine and Biological Sciences, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile.<br /><searchLink fieldCode="AU" term="%22Nuñez+I%22">Nuñez I</searchLink>; Agencia Nacional de Investigación y Desarrollo-Millennium Science Initiative Program-Millennium Institute for Integrative Biology (iBio), Schools of Engineering, Medicine and Biological Sciences, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile.; Institute for Biological and Medical Engineering, Schools of Engineering, Medicine and Biological Sciences, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile.<br /><searchLink fieldCode="AU" term="%22Cazaux+S%22">Cazaux S</searchLink>; Agencia Nacional de Investigación y Desarrollo-Millennium Science Initiative Program-Millennium Institute for Integrative Biology (iBio), Schools of Engineering, Medicine and Biological Sciences, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile.; Institute for Biological and Medical Engineering, Schools of Engineering, Medicine and Biological Sciences, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile.<br /><searchLink fieldCode="AU" term="%22Federici+F%22">Federici F</searchLink>; Agencia Nacional de Investigación y Desarrollo-Millennium Science Initiative Program-Millennium Institute for Integrative Biology (iBio), Schools of Engineering, Medicine and Biological Sciences, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile.; Institute for Biological and Medical Engineering, Schools of Engineering, Medicine and Biological Sciences, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile.<br /><searchLink fieldCode="AU" term="%22Moreno-Mendieta+K%22">Moreno-Mendieta K</searchLink>; Institute of Biochemistry and Microbiology, Faculty of Sciences, Universidad Austral de Chile, Valdivia 5110566, Chile.<br /><searchLink fieldCode="AU" term="%22Soto-Rauch+G%22">Soto-Rauch G</searchLink>; Institute of Biochemistry and Microbiology, Faculty of Sciences, Universidad Austral de Chile, Valdivia 5110566, Chile.<br /><searchLink fieldCode="AU" term="%22Castro+J%22">Castro J</searchLink>; Bain & Company, Santiago 7550268, Chile.<br /><searchLink fieldCode="AU" term="%22Frenkel+V%22">Frenkel V</searchLink>; Bain & Company, Santiago 7550268, Chile.<br /><searchLink fieldCode="AU" term="%22Ho+JH%22">Ho JH</searchLink>; Bain & Company, Santiago 7550268, Chile.<br /><searchLink fieldCode="AU" term="%22Ascencios+D%22">Ascencios D</searchLink>; Bain & Company, Santiago 7550268, Chile.<br /><searchLink fieldCode="AU" term="%22Sanhueza+Teneo+D%22">Sanhueza Teneo D</searchLink>; Institute of Immunology and Parasitology, Faculty of Medicine, Universidad Austral de Chile, Valdivia 5110566, Chile.<br /><searchLink fieldCode="AU" term="%22Munizaga+J%22">Munizaga J</searchLink>; Biochemistry School, Faculty of Sciences, Universidad Austral de Chile, Valdivia 5110566, Chile.; Facultad de Ciencias para el Cuidado de la Salud, Universidad San Sebastián, Valdivia 5090000, Chile.<br /><searchLink fieldCode="AU" term="%22Haussmann+D%22">Haussmann D</searchLink>; Departamento de Ciencias Básicas, Facultad de Ciencias, Universidad Santo Tomás, Valdivia 5110566, Chile.<br /><searchLink fieldCode="AU" term="%22Rojas-Fernandez+A%22">Rojas-Fernandez A</searchLink>; Institute of Medicine, Faculty of Medicine, Universidad Austral de Chile, Valdivia 5110566, Chile.; Berking Biotechnology, Valdivia 5090000, Chile.<br /><searchLink fieldCode="AU" term="%22Valverde+JF%22">Valverde JF</searchLink>; Institute of Biochemistry and Microbiology, Faculty of Sciences, Universidad Austral de Chile, Valdivia 5110566, Chile.; Interdisciplinary Center for Aquaculture Research (INCAR), Concepción 4030000, Chile.<br /><searchLink fieldCode="AU" term="%22Valenzuela-Nieto+G%22">Valenzuela-Nieto G</searchLink>; Facultad de Ciencias para el Cuidado de la Salud, Universidad San Sebastián, Valdivia 5090000, Chile.
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