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Simultaneous capture of single cell RNA-seq, ATAC-seq, and CRISPR perturbation enables multiomic screens to identify gene regulatory relationships.

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Title: Simultaneous capture of single cell RNA-seq, ATAC-seq, and CRISPR perturbation enables multiomic screens to identify gene regulatory relationships.
Authors: Shevade K; Laboratory for Genomics Research, San Francisco, CA 94158, USA; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA., Yang YA; Laboratory for Genomics Research, San Francisco, CA 94158, USA; GSK, San Francisco, CA, USA. Electronic address: yeqingyang2016@gmail.com., Feng K; Laboratory for Genomics Research, San Francisco, CA 94158, USA; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA., Mader K; Laboratory for Genomics Research, San Francisco, CA 94158, USA; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA., Sevim V; Laboratory for Genomics Research, San Francisco, CA 94158, USA; GSK, San Francisco, CA, USA., Parsons J; GSK, Collegeville, PA, USA., Arora G; Laboratory for Genomics Research, San Francisco, CA 94158, USA; GSK, San Francisco, CA, USA., Elfawy H; Laboratory for Genomics Research, San Francisco, CA 94158, USA; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA., Mace R; Laboratory for Genomics Research, San Francisco, CA 94158, USA; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA., Federman S; Laboratory for Genomics Research, San Francisco, CA 94158, USA; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA., Esanov R; Laboratory for Genomics Research, San Francisco, CA 94158, USA; GSK, San Francisco, CA, USA., Shafer S; Laboratory for Genomics Research, San Francisco, CA 94158, USA; GSK, San Francisco, CA, USA., Chow ED; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA; Center for Advanced Technology, University of California, San Francisco, CA 94158, USA. Electronic address: eric.chow@ucsf.edu., Przybyla L; Laboratory for Genomics Research, San Francisco, CA 94158, USA; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA.
Source: Cell reports methods [Cell Rep Methods] 2025 Dec 15; Vol. 5 (12), pp. 101222. Date of Electronic Publication: 2025 Nov 10.
Publication Type: Journal Article
Journal Info: Publisher: Elsevier Inc Country of Publication: United States NLM ID: 9918227360606676 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2667-2375 (Electronic) Linking ISSN: 26672375 NLM ISO Abbreviation: Cell Rep Methods Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: Simultaneous capture of single cell RNA-seq, ATAC-seq, and CRISPR perturbation enables multiomic screens to identify gene regulatory relationships.
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  Data: <searchLink fieldCode="AU" term="%22Shevade+K%22">Shevade K</searchLink>; Laboratory for Genomics Research, San Francisco, CA 94158, USA; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA.<br /><searchLink fieldCode="AU" term="%22Yang+YA%22">Yang YA</searchLink>; Laboratory for Genomics Research, San Francisco, CA 94158, USA; GSK, San Francisco, CA, USA. Electronic address: yeqingyang2016@gmail.com.<br /><searchLink fieldCode="AU" term="%22Feng+K%22">Feng K</searchLink>; Laboratory for Genomics Research, San Francisco, CA 94158, USA; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA.<br /><searchLink fieldCode="AU" term="%22Mader+K%22">Mader K</searchLink>; Laboratory for Genomics Research, San Francisco, CA 94158, USA; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA.<br /><searchLink fieldCode="AU" term="%22Sevim+V%22">Sevim V</searchLink>; Laboratory for Genomics Research, San Francisco, CA 94158, USA; GSK, San Francisco, CA, USA.<br /><searchLink fieldCode="AU" term="%22Parsons+J%22">Parsons J</searchLink>; GSK, Collegeville, PA, USA.<br /><searchLink fieldCode="AU" term="%22Arora+G%22">Arora G</searchLink>; Laboratory for Genomics Research, San Francisco, CA 94158, USA; GSK, San Francisco, CA, USA.<br /><searchLink fieldCode="AU" term="%22Elfawy+H%22">Elfawy H</searchLink>; Laboratory for Genomics Research, San Francisco, CA 94158, USA; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA.<br /><searchLink fieldCode="AU" term="%22Mace+R%22">Mace R</searchLink>; Laboratory for Genomics Research, San Francisco, CA 94158, USA; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA.<br /><searchLink fieldCode="AU" term="%22Federman+S%22">Federman S</searchLink>; Laboratory for Genomics Research, San Francisco, CA 94158, USA; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA.<br /><searchLink fieldCode="AU" term="%22Esanov+R%22">Esanov R</searchLink>; Laboratory for Genomics Research, San Francisco, CA 94158, USA; GSK, San Francisco, CA, USA.<br /><searchLink fieldCode="AU" term="%22Shafer+S%22">Shafer S</searchLink>; Laboratory for Genomics Research, San Francisco, CA 94158, USA; GSK, San Francisco, CA, USA.<br /><searchLink fieldCode="AU" term="%22Chow+ED%22">Chow ED</searchLink>; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA; Center for Advanced Technology, University of California, San Francisco, CA 94158, USA. Electronic address: eric.chow@ucsf.edu.<br /><searchLink fieldCode="AU" term="%22Przybyla+L%22">Przybyla L</searchLink>; Laboratory for Genomics Research, San Francisco, CA 94158, USA; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA.
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  Data: <searchLink fieldCode="JN" term="%229918227360606676%22">Cell reports methods</searchLink> [Cell Rep Methods] 2025 Dec 15; Vol. 5 (12), pp. 101222. <i>Date of Electronic Publication: </i>2025 Nov 10.
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              Text: 2025 Dec 15
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