Protocol for the expression, purification, and biochemical characterization of the innate immune sensor MDA5.
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| Title: | Protocol for the expression, purification, and biochemical characterization of the innate immune sensor MDA5. |
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| Authors: | Joiner JD; Molecular Immunity Unit, Department of Medicine, University of Cambridge, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK; Cambridge Institute of Therapeutic Immunology & Infectious Disease (CITIID), Department of Medicine, University of Cambridge, Cambridge CB2 0AW, UK. Electronic address: jjoiner@mrc-lmb.cam.ac.uk., Herrero Del Valle A; Molecular Immunity Unit, Department of Medicine, University of Cambridge, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK; Cambridge Institute of Therapeutic Immunology & Infectious Disease (CITIID), Department of Medicine, University of Cambridge, Cambridge CB2 0AW, UK., Singh R; Molecular Immunity Unit, Department of Medicine, University of Cambridge, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK; Cambridge Institute of Therapeutic Immunology & Infectious Disease (CITIID), Department of Medicine, University of Cambridge, Cambridge CB2 0AW, UK; Department of Pathology, University of Cambridge, Cambridge CB2 1QP, UK., Modis Y; Molecular Immunity Unit, Department of Medicine, University of Cambridge, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK; Cambridge Institute of Therapeutic Immunology & Infectious Disease (CITIID), Department of Medicine, University of Cambridge, Cambridge CB2 0AW, UK. Electronic address: ymodis@mrc-lmb.cam.ac.uk. |
| Source: | STAR protocols [STAR Protoc] 2025 Dec 19; Vol. 6 (4), pp. 104218. Date of Electronic Publication: 2025 Nov 20. |
| Publication Type: | Journal Article |
| Journal Info: | Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE |
| Database: | MEDLINE Ultimate |
| FullText | Text: Availability: 0 |
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| Header | DbId: mdl DbLabel: MEDLINE Ultimate An: 41269861 AccessLevel: 2 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Protocol for the expression, purification, and biochemical characterization of the innate immune sensor MDA5. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AU" term="%22Joiner+JD%22">Joiner JD</searchLink>; Molecular Immunity Unit, Department of Medicine, University of Cambridge, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK; Cambridge Institute of Therapeutic Immunology & Infectious Disease (CITIID), Department of Medicine, University of Cambridge, Cambridge CB2 0AW, UK. Electronic address: jjoiner@mrc-lmb.cam.ac.uk.<br /><searchLink fieldCode="AU" term="%22Herrero+Del+Valle+A%22">Herrero Del Valle A</searchLink>; Molecular Immunity Unit, Department of Medicine, University of Cambridge, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK; Cambridge Institute of Therapeutic Immunology & Infectious Disease (CITIID), Department of Medicine, University of Cambridge, Cambridge CB2 0AW, UK.<br /><searchLink fieldCode="AU" term="%22Singh+R%22">Singh R</searchLink>; Molecular Immunity Unit, Department of Medicine, University of Cambridge, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK; Cambridge Institute of Therapeutic Immunology & Infectious Disease (CITIID), Department of Medicine, University of Cambridge, Cambridge CB2 0AW, UK; Department of Pathology, University of Cambridge, Cambridge CB2 1QP, UK.<br /><searchLink fieldCode="AU" term="%22Modis+Y%22">Modis Y</searchLink>; Molecular Immunity Unit, Department of Medicine, University of Cambridge, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK; Cambridge Institute of Therapeutic Immunology & Infectious Disease (CITIID), Department of Medicine, University of Cambridge, Cambridge CB2 0AW, UK. Electronic address: ymodis@mrc-lmb.cam.ac.uk. – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22101769501%22">STAR protocols</searchLink> [STAR Protoc] 2025 Dec 19; Vol. 6 (4), pp. 104218. <i>Date of Electronic Publication: </i>2025 Nov 20. – Name: TypePub Label: Publication Type Group: TypPub Data: Journal Article – Name: TitleSource Label: Journal Info Group: Src Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Cell+Press%22">Cell Press </searchLink><i>Country of Publication: </i>United States <i>NLM ID: </i>101769501 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>2666-1667 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2226661667%22">26661667 </searchLink><i>NLM ISO Abbreviation: </i>STAR Protoc <i>Subsets: </i>MEDLINE |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=mdl&AN=41269861 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.xpro.2025.104218 Languages: – Code: eng Text: English PhysicalDescription: Pagination: StartPage: 104218 Titles: – TitleFull: Protocol for the expression, purification, and biochemical characterization of the innate immune sensor MDA5. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Joiner JD – PersonEntity: Name: NameFull: Herrero Del Valle A – PersonEntity: Name: NameFull: Singh R – PersonEntity: Name: NameFull: Modis Y IsPartOfRelationships: – BibEntity: Dates: – D: 19 M: 12 Text: 2025 Dec 19 Type: published Y: 2025 Identifiers: – Type: issn-electronic Value: 2666-1667 Numbering: – Type: volume Value: 6 – Type: issue Value: 4 Titles: – TitleFull: STAR protocols Type: main |
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