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A combined PIE-FRET and FCS assay to monitor RNA dynamics and cleavage by SARS-CoV-2 Nsp15.

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Title: A combined PIE-FRET and FCS assay to monitor RNA dynamics and cleavage by SARS-CoV-2 Nsp15.
Authors: Gordon K; Department of Physics, North Carolina State University, Raleigh, NC 27695-8202, USA., Wright Z; Molecular and Cellular Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, 111 T. W. Alexander Drive, Research Triangle Park, NC 27709, USA., Frazier M; Molecular and Cellular Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, 111 T. W. Alexander Drive, Research Triangle Park, NC 27709, USA.; Department of Chemistry and Biochemistry, College of Charleston, 66 George St., Charleston, SC 29424, USA., Clark BS; Department of Physics, North Carolina State University, Raleigh, NC 27695-8202, USA., Wilson I; Molecular and Cellular Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, 111 T. W. Alexander Drive, Research Triangle Park, NC 27709, USA.; Current Affiliation: Howard University College of Medicine, Washington, DC 20059, USA., Stephens CR; Department of Physics, North Carolina State University, Raleigh, NC 27695-8202, USA., Silvernail I; Department of Physics, North Carolina State University, Raleigh, NC 27695-8202, USA., Stanley RE; Molecular and Cellular Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, 111 T. W. Alexander Drive, Research Triangle Park, NC 27709, USA., LeBlanc SJ; Department of Physics, North Carolina State University, Raleigh, NC 27695-8202, USA.
Source: Research square [Res Sq] 2025 Oct 09. Date of Electronic Publication: 2025 Oct 09.
Publication Type: Journal Article; Preprint
Journal Info: Country of Publication: United States NLM ID: 101768035 Publication Model: Electronic Cited Medium: Internet ISSN: 2693-5015 (Electronic) Linking ISSN: 26935015 NLM ISO Abbreviation: Res Sq Subsets: PubMed not MEDLINE
Database: MEDLINE Ultimate
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        Value: 10.21203/rs.3.rs-7717808/v1
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        Text: English
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              Text: 2025 Oct 09
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