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Optimizing Tissue Lysis and DNA Extraction Protocols to Enhance Bacterial Diversity Profiling in the Drosophila melanogaster Gut Microbiome.

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Title: Optimizing Tissue Lysis and DNA Extraction Protocols to Enhance Bacterial Diversity Profiling in the Drosophila melanogaster Gut Microbiome.
Authors: Quiñones-Sanchez CL; Department of Biology, College of Natural Sciences, University of Puerto Rico, Rio Piedras, San Juan 00925, Puerto Rico., Bilbao-Del Valle JL; Department of Biology, College of Natural Sciences, University of Puerto Rico, Rio Piedras, San Juan 00925, Puerto Rico., Urdaneta-Colon MA; Department of Biology, College of Natural Sciences, University of Puerto Rico, Rio Piedras, San Juan 00925, Puerto Rico., Santiago-Rodriguez TM; The Alkek Center for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA.; Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA., Rodriguez-Fernandez IA; Department of Biology, College of Natural Sciences, University of Puerto Rico, Rio Piedras, San Juan 00925, Puerto Rico.
Source: BioRxiv : the preprint server for biology [bioRxiv] 2025 Nov 15. Date of Electronic Publication: 2025 Nov 15.
Publication Type: Journal Article; Preprint
Journal Info: Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet ISSN: 2692-8205 (Electronic) Linking ISSN: 26928205 NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE
Database: MEDLINE Ultimate
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PubType: Academic Journal
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  Data: Optimizing Tissue Lysis and DNA Extraction Protocols to Enhance Bacterial Diversity Profiling in the Drosophila melanogaster Gut Microbiome.
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  Data: <searchLink fieldCode="AU" term="%22Quiñones-Sanchez+CL%22">Quiñones-Sanchez CL</searchLink>; Department of Biology, College of Natural Sciences, University of Puerto Rico, Rio Piedras, San Juan 00925, Puerto Rico.<br /><searchLink fieldCode="AU" term="%22Bilbao-Del+Valle+JL%22">Bilbao-Del Valle JL</searchLink>; Department of Biology, College of Natural Sciences, University of Puerto Rico, Rio Piedras, San Juan 00925, Puerto Rico.<br /><searchLink fieldCode="AU" term="%22Urdaneta-Colon+MA%22">Urdaneta-Colon MA</searchLink>; Department of Biology, College of Natural Sciences, University of Puerto Rico, Rio Piedras, San Juan 00925, Puerto Rico.<br /><searchLink fieldCode="AU" term="%22Santiago-Rodriguez+TM%22">Santiago-Rodriguez TM</searchLink>; The Alkek Center for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA.; Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA.<br /><searchLink fieldCode="AU" term="%22Rodriguez-Fernandez+IA%22">Rodriguez-Fernandez IA</searchLink>; Department of Biology, College of Natural Sciences, University of Puerto Rico, Rio Piedras, San Juan 00925, Puerto Rico.
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  Data: <searchLink fieldCode="JN" term="%22101680187%22">BioRxiv : the preprint server for biology</searchLink> [bioRxiv] 2025 Nov 15. <i>Date of Electronic Publication: </i>2025 Nov 15.
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        Value: 10.1101/2025.11.15.688634
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              Text: 2025 Nov 15
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