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Development and Validation of a Commercial TaqMan-Based RT-qPCR Kit for Rotavirus and Norovirus Detection in the Brazilian Acute Diarrhea Surveillance Network.

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Title: Development and Validation of a Commercial TaqMan-Based RT-qPCR Kit for Rotavirus and Norovirus Detection in the Brazilian Acute Diarrhea Surveillance Network.
Authors: Cambri G; Molecular Biology Institute of Paraná (IBMP), Curitiba 81350-010, PR, Brazil., Jacomasso T; Molecular Biology Institute of Paraná (IBMP), Curitiba 81350-010, PR, Brazil., Burlandy FM; Laboratory of Comparative and Environmental Virology (LVCA), Oswaldo Cruz Institute, Oswaldo Cruz Foundation (Fiocruz), Av. Brasil 4365, Rio de Janeiro 21040-360, RJ, Brazil., Malta FC; Laboratory of Comparative and Environmental Virology (LVCA), Oswaldo Cruz Institute, Oswaldo Cruz Foundation (Fiocruz), Av. Brasil 4365, Rio de Janeiro 21040-360, RJ, Brazil., Fialho AM; Laboratory of Comparative and Environmental Virology (LVCA), Oswaldo Cruz Institute, Oswaldo Cruz Foundation (Fiocruz), Av. Brasil 4365, Rio de Janeiro 21040-360, RJ, Brazil., Cilli A; Enteric Diseases Laboratory (LDE), Virology Center (CV), Adolfo Lutz Institute (IAL), Av. Dr Arnaldo, 355, São Paulo 01246-900, SP, Brazil., Guadagnucci S; Enteric Diseases Laboratory (LDE), Virology Center (CV), Adolfo Lutz Institute (IAL), Av. Dr Arnaldo, 355, São Paulo 01246-900, SP, Brazil., Teixeira DM; Laboratory of Gastroenteric Viruses (LVG), Virology Section, Evandro Chagas Institute, Secretariat of Health Surveillance and Environment, Brazilian Ministry of Health, Ananindeua 67030-000, PA, Brazil., Lobo PS; Laboratory of Gastroenteric Viruses (LVG), Virology Section, Evandro Chagas Institute, Secretariat of Health Surveillance and Environment, Brazilian Ministry of Health, Ananindeua 67030-000, PA, Brazil., Resque HR; Laboratory of Gastroenteric Viruses (LVG), Virology Section, Evandro Chagas Institute, Secretariat of Health Surveillance and Environment, Brazilian Ministry of Health, Ananindeua 67030-000, PA, Brazil., Berto LH; General Coordination of Public Health Laboratories (CGLab), Ministry of Health, Federal District, Brasília 70058-900, DF, Brazil., Afornali A; Molecular Biology Institute of Paraná (IBMP), Curitiba 81350-010, PR, Brazil., Marchini FK; Molecular Biology Institute of Paraná (IBMP), Curitiba 81350-010, PR, Brazil.; Laboratory for Applied Science and Technology in Health, Carlos Chagas Institute, Oswaldo Cruz Foundation (Fiocruz-PR), Curitiba 81350-010, PR, Brazil., Riediger IN; Molecular Biology Institute of Paraná (IBMP), Curitiba 81350-010, PR, Brazil., Soares LS; Laboratory of Gastroenteric Viruses (LVG), Virology Section, Evandro Chagas Institute, Secretariat of Health Surveillance and Environment, Brazilian Ministry of Health, Ananindeua 67030-000, PA, Brazil., Carmona RCC; Enteric Diseases Laboratory (LDE), Virology Center (CV), Adolfo Lutz Institute (IAL), Av. Dr Arnaldo, 355, São Paulo 01246-900, SP, Brazil., Fumian TM; Laboratory of Comparative and Environmental Virology (LVCA), Oswaldo Cruz Institute, Oswaldo Cruz Foundation (Fiocruz), Av. Brasil 4365, Rio de Janeiro 21040-360, RJ, Brazil.
Source: Viruses [Viruses] 2025 Nov 28; Vol. 17 (12). Date of Electronic Publication: 2025 Nov 28.
Publication Type: Journal Article; Validation Study; Multicenter Study
Journal Info: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101509722 Publication Model: Electronic Cited Medium: Internet ISSN: 1999-4915 (Electronic) Linking ISSN: 19994915 NLM ISO Abbreviation: Viruses Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Label: Title
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  Data: Development and Validation of a Commercial TaqMan-Based RT-qPCR Kit for Rotavirus and Norovirus Detection in the Brazilian Acute Diarrhea Surveillance Network.
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  Data: <searchLink fieldCode="AU" term="%22Cambri+G%22">Cambri G</searchLink>; Molecular Biology Institute of Paraná (IBMP), Curitiba 81350-010, PR, Brazil.<br /><searchLink fieldCode="AU" term="%22Jacomasso+T%22">Jacomasso T</searchLink>; Molecular Biology Institute of Paraná (IBMP), Curitiba 81350-010, PR, Brazil.<br /><searchLink fieldCode="AU" term="%22Burlandy+FM%22">Burlandy FM</searchLink>; Laboratory of Comparative and Environmental Virology (LVCA), Oswaldo Cruz Institute, Oswaldo Cruz Foundation (Fiocruz), Av. Brasil 4365, Rio de Janeiro 21040-360, RJ, Brazil.<br /><searchLink fieldCode="AU" term="%22Malta+FC%22">Malta FC</searchLink>; Laboratory of Comparative and Environmental Virology (LVCA), Oswaldo Cruz Institute, Oswaldo Cruz Foundation (Fiocruz), Av. Brasil 4365, Rio de Janeiro 21040-360, RJ, Brazil.<br /><searchLink fieldCode="AU" term="%22Fialho+AM%22">Fialho AM</searchLink>; Laboratory of Comparative and Environmental Virology (LVCA), Oswaldo Cruz Institute, Oswaldo Cruz Foundation (Fiocruz), Av. Brasil 4365, Rio de Janeiro 21040-360, RJ, Brazil.<br /><searchLink fieldCode="AU" term="%22Cilli+A%22">Cilli A</searchLink>; Enteric Diseases Laboratory (LDE), Virology Center (CV), Adolfo Lutz Institute (IAL), Av. Dr Arnaldo, 355, São Paulo 01246-900, SP, Brazil.<br /><searchLink fieldCode="AU" term="%22Guadagnucci+S%22">Guadagnucci S</searchLink>; Enteric Diseases Laboratory (LDE), Virology Center (CV), Adolfo Lutz Institute (IAL), Av. Dr Arnaldo, 355, São Paulo 01246-900, SP, Brazil.<br /><searchLink fieldCode="AU" term="%22Teixeira+DM%22">Teixeira DM</searchLink>; Laboratory of Gastroenteric Viruses (LVG), Virology Section, Evandro Chagas Institute, Secretariat of Health Surveillance and Environment, Brazilian Ministry of Health, Ananindeua 67030-000, PA, Brazil.<br /><searchLink fieldCode="AU" term="%22Lobo+PS%22">Lobo PS</searchLink>; Laboratory of Gastroenteric Viruses (LVG), Virology Section, Evandro Chagas Institute, Secretariat of Health Surveillance and Environment, Brazilian Ministry of Health, Ananindeua 67030-000, PA, Brazil.<br /><searchLink fieldCode="AU" term="%22Resque+HR%22">Resque HR</searchLink>; Laboratory of Gastroenteric Viruses (LVG), Virology Section, Evandro Chagas Institute, Secretariat of Health Surveillance and Environment, Brazilian Ministry of Health, Ananindeua 67030-000, PA, Brazil.<br /><searchLink fieldCode="AU" term="%22Berto+LH%22">Berto LH</searchLink>; General Coordination of Public Health Laboratories (CGLab), Ministry of Health, Federal District, Brasília 70058-900, DF, Brazil.<br /><searchLink fieldCode="AU" term="%22Afornali+A%22">Afornali A</searchLink>; Molecular Biology Institute of Paraná (IBMP), Curitiba 81350-010, PR, Brazil.<br /><searchLink fieldCode="AU" term="%22Marchini+FK%22">Marchini FK</searchLink>; Molecular Biology Institute of Paraná (IBMP), Curitiba 81350-010, PR, Brazil.; Laboratory for Applied Science and Technology in Health, Carlos Chagas Institute, Oswaldo Cruz Foundation (Fiocruz-PR), Curitiba 81350-010, PR, Brazil.<br /><searchLink fieldCode="AU" term="%22Riediger+IN%22">Riediger IN</searchLink>; Molecular Biology Institute of Paraná (IBMP), Curitiba 81350-010, PR, Brazil.<br /><searchLink fieldCode="AU" term="%22Soares+LS%22">Soares LS</searchLink>; Laboratory of Gastroenteric Viruses (LVG), Virology Section, Evandro Chagas Institute, Secretariat of Health Surveillance and Environment, Brazilian Ministry of Health, Ananindeua 67030-000, PA, Brazil.<br /><searchLink fieldCode="AU" term="%22Carmona+RCC%22">Carmona RCC</searchLink>; Enteric Diseases Laboratory (LDE), Virology Center (CV), Adolfo Lutz Institute (IAL), Av. Dr Arnaldo, 355, São Paulo 01246-900, SP, Brazil.<br /><searchLink fieldCode="AU" term="%22Fumian+TM%22">Fumian TM</searchLink>; Laboratory of Comparative and Environmental Virology (LVCA), Oswaldo Cruz Institute, Oswaldo Cruz Foundation (Fiocruz), Av. Brasil 4365, Rio de Janeiro 21040-360, RJ, Brazil.
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  Data: <searchLink fieldCode="JN" term="%22101509722%22">Viruses</searchLink> [Viruses] 2025 Nov 28; Vol. 17 (12). <i>Date of Electronic Publication: </i>2025 Nov 28.
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  Data: Journal Article; Validation Study; Multicenter Study
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22MDPI%22">MDPI </searchLink><i>Country of Publication: </i>Switzerland <i>NLM ID: </i>101509722 <i>Publication Model: </i>Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1999-4915 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2219994915%22">19994915 </searchLink><i>NLM ISO Abbreviation: </i>Viruses <i>Subsets: </i>MEDLINE
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              Text: 2025 Nov 28
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