Connector-enabled integration of Golden Gate Assembly and yeast recombination for streamlined multigene pathway construction in the biofoundry workflow.
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| Title: | Connector-enabled integration of Golden Gate Assembly and yeast recombination for streamlined multigene pathway construction in the biofoundry workflow. |
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| Authors: | Seong MJ; Synthetic Biology Research Center and Korea Biofoundry, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea; Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon 34113, Republic of Korea., Kim H; Synthetic Biology Research Center and Korea Biofoundry, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea., Lee H; Synthetic Biology Research Center and Korea Biofoundry, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea; Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon 34113, Republic of Korea., Kim H; Synthetic Biology Research Center and Korea Biofoundry, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea; Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon 34113, Republic of Korea; Graduate School of Engineering Biology, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea., Lee SG; Synthetic Biology Research Center and Korea Biofoundry, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea; Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon 34113, Republic of Korea; Graduate School of Engineering Biology, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea., Lee MJ; Synthetic Biology Research Center and Korea Biofoundry, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea., Jun JS; Synthetic Biology Research Center and Korea Biofoundry, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea., Hong S; Synthetic Biology Research Center and Korea Biofoundry, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea., Kim TH; Synthetic Biology Research Center and Korea Biofoundry, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea., Kim SK; Synthetic Biology Research Center and Korea Biofoundry, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea., Shin J; Synthetic Biology Research Center and Korea Biofoundry, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea; Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon 34113, Republic of Korea; Department of Food Science and Biotechnology, College of Life Sciences, Sejong University, Seoul 05006, Republic of Korea; Carbohydrate Bioproduct Research Center, Sejong University, Seoul 05006, Republic of Korea. Electronic address: jhshin@sejong.ac.kr., Lee DH; Synthetic Biology Research Center and Korea Biofoundry, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea; Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon 34113, Republic of Korea; Graduate School of Engineering Biology, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea; Department of Integrative Biotechnology, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon-si, Gyeonggi-do 16419, Republic of Korea. Electronic address: dhlee@kribb.re.kr. |
| Source: | Trends in biotechnology [Trends Biotechnol] 2026 May; Vol. 44 (5), pp. 1478-1497. Date of Electronic Publication: 2026 Jan 14. |
| Publication Type: | Journal Article |
| Journal Info: | Publisher: Elsevier Science Publishers Country of Publication: England NLM ID: 8310903 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3096 (Electronic) Linking ISSN: 01677799 NLM ISO Abbreviation: Trends Biotechnol Subsets: MEDLINE |
| Database: | MEDLINE Ultimate |
| FullText | Text: Availability: 0 |
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| Header | DbId: mdl DbLabel: MEDLINE Ultimate An: 41535170 AccessLevel: 2 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Connector-enabled integration of Golden Gate Assembly and yeast recombination for streamlined multigene pathway construction in the biofoundry workflow. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AU" term="%22Seong+MJ%22">Seong MJ</searchLink>; Synthetic Biology Research Center and Korea Biofoundry, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea; Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon 34113, Republic of Korea.<br /><searchLink fieldCode="AU" term="%22Kim+H%22">Kim H</searchLink>; Synthetic Biology Research Center and Korea Biofoundry, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.<br /><searchLink fieldCode="AU" term="%22Lee+H%22">Lee H</searchLink>; Synthetic Biology Research Center and Korea Biofoundry, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea; Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon 34113, Republic of Korea.<br /><searchLink fieldCode="AU" term="%22Kim+H%22">Kim H</searchLink>; Synthetic Biology Research Center and Korea Biofoundry, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea; Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon 34113, Republic of Korea; Graduate School of Engineering Biology, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.<br /><searchLink fieldCode="AU" term="%22Lee+SG%22">Lee SG</searchLink>; Synthetic Biology Research Center and Korea Biofoundry, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea; Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon 34113, Republic of Korea; Graduate School of Engineering Biology, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.<br /><searchLink fieldCode="AU" term="%22Lee+MJ%22">Lee MJ</searchLink>; Synthetic Biology Research Center and Korea Biofoundry, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.<br /><searchLink fieldCode="AU" term="%22Jun+JS%22">Jun JS</searchLink>; Synthetic Biology Research Center and Korea Biofoundry, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.<br /><searchLink fieldCode="AU" term="%22Hong+S%22">Hong S</searchLink>; Synthetic Biology Research Center and Korea Biofoundry, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.<br /><searchLink fieldCode="AU" term="%22Kim+TH%22">Kim TH</searchLink>; Synthetic Biology Research Center and Korea Biofoundry, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.<br /><searchLink fieldCode="AU" term="%22Kim+SK%22">Kim SK</searchLink>; Synthetic Biology Research Center and Korea Biofoundry, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.<br /><searchLink fieldCode="AU" term="%22Shin+J%22">Shin J</searchLink>; Synthetic Biology Research Center and Korea Biofoundry, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea; Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon 34113, Republic of Korea; Department of Food Science and Biotechnology, College of Life Sciences, Sejong University, Seoul 05006, Republic of Korea; Carbohydrate Bioproduct Research Center, Sejong University, Seoul 05006, Republic of Korea. Electronic address: jhshin@sejong.ac.kr.<br /><searchLink fieldCode="AU" term="%22Lee+DH%22">Lee DH</searchLink>; Synthetic Biology Research Center and Korea Biofoundry, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea; Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon 34113, Republic of Korea; Graduate School of Engineering Biology, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea; Department of Integrative Biotechnology, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon-si, Gyeonggi-do 16419, Republic of Korea. Electronic address: dhlee@kribb.re.kr. – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%228310903%22">Trends in biotechnology</searchLink> [Trends Biotechnol] 2026 May; Vol. 44 (5), pp. 1478-1497. <i>Date of Electronic Publication: </i>2026 Jan 14. – Name: TypePub Label: Publication Type Group: TypPub Data: Journal Article – Name: TitleSource Label: Journal Info Group: Src Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Elsevier+Science+Publishers%22">Elsevier Science Publishers </searchLink><i>Country of Publication: </i>England <i>NLM ID: </i>8310903 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1879-3096 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2201677799%22">01677799 </searchLink><i>NLM ISO Abbreviation: </i>Trends Biotechnol <i>Subsets: </i>MEDLINE |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.tibtech.2025.12.004 Languages: – Code: eng Text: English PhysicalDescription: Pagination: StartPage: 1478 Titles: – TitleFull: Connector-enabled integration of Golden Gate Assembly and yeast recombination for streamlined multigene pathway construction in the biofoundry workflow. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Seong MJ – PersonEntity: Name: NameFull: Kim H – PersonEntity: Name: NameFull: Lee H – PersonEntity: Name: NameFull: Kim H – PersonEntity: Name: NameFull: Lee SG – PersonEntity: Name: NameFull: Lee MJ – PersonEntity: Name: NameFull: Jun JS – PersonEntity: Name: NameFull: Hong S – PersonEntity: Name: NameFull: Kim TH – PersonEntity: Name: NameFull: Kim SK – PersonEntity: Name: NameFull: Shin J – PersonEntity: Name: NameFull: Lee DH IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 05 Text: 2026 May Type: published Y: 2026 Identifiers: – Type: issn-electronic Value: 1879-3096 Numbering: – Type: volume Value: 44 – Type: issue Value: 5 Titles: – TitleFull: Trends in biotechnology Type: main |
| ResultId | 1 |