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Atypical GPCR Activation Resolved by Nanobody Engineering.

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Title: Atypical GPCR Activation Resolved by Nanobody Engineering.
Authors: Schlimgen RR; Department of Biochemistry, Medical College of Wisconsin, Milwaukee, USA.; Department of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, CO, USA., Jenjak SE; Department of Biochemistry, Medical College of Wisconsin, Milwaukee, USA., De La Sancha A; Department of Biochemistry, Medical College of Wisconsin, Milwaukee, USA., Darcis J; Immuno-Pharmacology and Interactomics, Luxembourg Institute of Health (LIH), Esch-sur-Alzette, Luxembourg.; Faculty of Science, Technology and Medicine, University of Luxembourg, 2, place de l'Université, L-4365 Esch-sur-Alzette, Luxembourg., Billesbølle CB; Department of Pharmaceutical Chemistry, University of California, San Francisco, CA, USA., Olson LJ; Department of Biophysics, Medical College of Wisconsin, Milwaukee, USA., Peterson FC; Department of Biochemistry, Medical College of Wisconsin, Milwaukee, USA., Smit MJ; Amsterdam Institute of Molecular and Life Sciences, Department of Chemistry and Pharmaceutical Sciences, Faculty of Sciences, Vrije Universiteit, 1081 HZ Amsterdam, The Netherlands., Manglik A; Department of Pharmaceutical Chemistry, University of California, San Francisco, CA, USA.; Department of Anesthesia and Perioperative Care, University of California, San Francisco, CA, USA.; Chan Zuckerberg Biohub, San Francisco, CA, USA., Szpakowska M; Immuno-Pharmacology and Interactomics, Luxembourg Institute of Health (LIH), Esch-sur-Alzette, Luxembourg., Chevigne A; Immuno-Pharmacology and Interactomics, Luxembourg Institute of Health (LIH), Esch-sur-Alzette, Luxembourg., Volkman BF; Department of Biochemistry, Medical College of Wisconsin, Milwaukee, USA.; Program in Chemical Biology, Medical College of Wisconsin, Milwaukee, Wisconsin.
Source: BioRxiv : the preprint server for biology [bioRxiv] 2026 Jan 11. Date of Electronic Publication: 2026 Jan 11.
Publication Type: Journal Article; Preprint
Journal Info: Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet ISSN: 2692-8205 (Electronic) Linking ISSN: 26928205 NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE
Database: MEDLINE Ultimate
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  Data: <searchLink fieldCode="AU" term="%22Schlimgen+RR%22">Schlimgen RR</searchLink>; Department of Biochemistry, Medical College of Wisconsin, Milwaukee, USA.; Department of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.<br /><searchLink fieldCode="AU" term="%22Jenjak+SE%22">Jenjak SE</searchLink>; Department of Biochemistry, Medical College of Wisconsin, Milwaukee, USA.<br /><searchLink fieldCode="AU" term="%22De+La+Sancha+A%22">De La Sancha A</searchLink>; Department of Biochemistry, Medical College of Wisconsin, Milwaukee, USA.<br /><searchLink fieldCode="AU" term="%22Darcis+J%22">Darcis J</searchLink>; Immuno-Pharmacology and Interactomics, Luxembourg Institute of Health (LIH), Esch-sur-Alzette, Luxembourg.; Faculty of Science, Technology and Medicine, University of Luxembourg, 2, place de l'Université, L-4365 Esch-sur-Alzette, Luxembourg.<br /><searchLink fieldCode="AU" term="%22Billesbølle+CB%22">Billesbølle CB</searchLink>; Department of Pharmaceutical Chemistry, University of California, San Francisco, CA, USA.<br /><searchLink fieldCode="AU" term="%22Olson+LJ%22">Olson LJ</searchLink>; Department of Biophysics, Medical College of Wisconsin, Milwaukee, USA.<br /><searchLink fieldCode="AU" term="%22Peterson+FC%22">Peterson FC</searchLink>; Department of Biochemistry, Medical College of Wisconsin, Milwaukee, USA.<br /><searchLink fieldCode="AU" term="%22Smit+MJ%22">Smit MJ</searchLink>; Amsterdam Institute of Molecular and Life Sciences, Department of Chemistry and Pharmaceutical Sciences, Faculty of Sciences, Vrije Universiteit, 1081 HZ Amsterdam, The Netherlands.<br /><searchLink fieldCode="AU" term="%22Manglik+A%22">Manglik A</searchLink>; Department of Pharmaceutical Chemistry, University of California, San Francisco, CA, USA.; Department of Anesthesia and Perioperative Care, University of California, San Francisco, CA, USA.; Chan Zuckerberg Biohub, San Francisco, CA, USA.<br /><searchLink fieldCode="AU" term="%22Szpakowska+M%22">Szpakowska M</searchLink>; Immuno-Pharmacology and Interactomics, Luxembourg Institute of Health (LIH), Esch-sur-Alzette, Luxembourg.<br /><searchLink fieldCode="AU" term="%22Chevigne+A%22">Chevigne A</searchLink>; Immuno-Pharmacology and Interactomics, Luxembourg Institute of Health (LIH), Esch-sur-Alzette, Luxembourg.<br /><searchLink fieldCode="AU" term="%22Volkman+BF%22">Volkman BF</searchLink>; Department of Biochemistry, Medical College of Wisconsin, Milwaukee, USA.; Program in Chemical Biology, Medical College of Wisconsin, Milwaukee, Wisconsin.
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