Single-molecule validation and optimised protocols for the use of secondary nanobodies in multiplexed immunoassays.

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Bibliographic Details
Title: Single-molecule validation and optimised protocols for the use of secondary nanobodies in multiplexed immunoassays.
Authors: Saleeb R; EaStCHEM School of Chemistry, The University of Edinburgh, Edinburgh, UK.; Institute for Regeneration and Repair, The University of Edinburgh, Edinburgh, UK., O'Shaughnessy J; EaStCHEM School of Chemistry, The University of Edinburgh, Edinburgh, UK.; Institute for Regeneration and Repair, The University of Edinburgh, Edinburgh, UK., Ferguson R; EaStCHEM School of Chemistry, The University of Edinburgh, Edinburgh, UK.; Institute for Regeneration and Repair, The University of Edinburgh, Edinburgh, UK., Adams CT; EaStCHEM School of Chemistry, The University of Edinburgh, Edinburgh, UK., Horrocks MH; EaStCHEM School of Chemistry, The University of Edinburgh, Edinburgh, UK.; Institute for Regeneration and Repair, The University of Edinburgh, Edinburgh, UK.
Source: Journal of microscopy [J Microsc] 2026 Mar; Vol. 301 (3), pp. 422-436. Date of Electronic Publication: 2026 Jan 21.
Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural
Journal Info: Publisher: Published for the Royal Microscopical Society by Blackwell Scientific Publications Country of Publication: England NLM ID: 0204522 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2818 (Electronic) Linking ISSN: 00222720 NLM ISO Abbreviation: J Microsc Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:1365-2818
DOI:10.1111/jmi.70059