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Single-molecule validation and optimised protocols for the use of secondary nanobodies in multiplexed immunoassays.

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Title: Single-molecule validation and optimised protocols for the use of secondary nanobodies in multiplexed immunoassays.
Authors: Saleeb R; EaStCHEM School of Chemistry, The University of Edinburgh, Edinburgh, UK.; Institute for Regeneration and Repair, The University of Edinburgh, Edinburgh, UK., O'Shaughnessy J; EaStCHEM School of Chemistry, The University of Edinburgh, Edinburgh, UK.; Institute for Regeneration and Repair, The University of Edinburgh, Edinburgh, UK., Ferguson R; EaStCHEM School of Chemistry, The University of Edinburgh, Edinburgh, UK.; Institute for Regeneration and Repair, The University of Edinburgh, Edinburgh, UK., Adams CT; EaStCHEM School of Chemistry, The University of Edinburgh, Edinburgh, UK., Horrocks MH; EaStCHEM School of Chemistry, The University of Edinburgh, Edinburgh, UK.; Institute for Regeneration and Repair, The University of Edinburgh, Edinburgh, UK.
Source: Journal of microscopy [J Microsc] 2026 Mar; Vol. 301 (3), pp. 422-436. Date of Electronic Publication: 2026 Jan 21.
Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural
Journal Info: Publisher: Published for the Royal Microscopical Society by Blackwell Scientific Publications Country of Publication: England NLM ID: 0204522 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2818 (Electronic) Linking ISSN: 00222720 NLM ISO Abbreviation: J Microsc Subsets: MEDLINE
Database: MEDLINE Ultimate
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PubType: Academic Journal
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  Data: <searchLink fieldCode="AU" term="%22Saleeb+R%22">Saleeb R</searchLink>; EaStCHEM School of Chemistry, The University of Edinburgh, Edinburgh, UK.; Institute for Regeneration and Repair, The University of Edinburgh, Edinburgh, UK.<br /><searchLink fieldCode="AU" term="%22O'Shaughnessy+J%22">O'Shaughnessy J</searchLink>; EaStCHEM School of Chemistry, The University of Edinburgh, Edinburgh, UK.; Institute for Regeneration and Repair, The University of Edinburgh, Edinburgh, UK.<br /><searchLink fieldCode="AU" term="%22Ferguson+R%22">Ferguson R</searchLink>; EaStCHEM School of Chemistry, The University of Edinburgh, Edinburgh, UK.; Institute for Regeneration and Repair, The University of Edinburgh, Edinburgh, UK.<br /><searchLink fieldCode="AU" term="%22Adams+CT%22">Adams CT</searchLink>; EaStCHEM School of Chemistry, The University of Edinburgh, Edinburgh, UK.<br /><searchLink fieldCode="AU" term="%22Horrocks+MH%22">Horrocks MH</searchLink>; EaStCHEM School of Chemistry, The University of Edinburgh, Edinburgh, UK.; Institute for Regeneration and Repair, The University of Edinburgh, Edinburgh, UK.
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  Data: <searchLink fieldCode="JN" term="%220204522%22">Journal of microscopy</searchLink> [J Microsc] 2026 Mar; Vol. 301 (3), pp. 422-436. <i>Date of Electronic Publication: </i>2026 Jan 21.
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  Data: Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural
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        Value: 10.1111/jmi.70059
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      – Code: eng
        Text: English
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            NameFull: Saleeb R
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            – D: 01
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              Text: 2026 Mar
              Type: published
              Y: 2026
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