Diagnostic Concordance of Two- and Three-Gene SARS-CoV-2 Molecular Assays in Cameroon: Implications for Efficient Pandemic Response in Low- and Middle-Income Countries.

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Title: Diagnostic Concordance of Two- and Three-Gene SARS-CoV-2 Molecular Assays in Cameroon: Implications for Efficient Pandemic Response in Low- and Middle-Income Countries.
Authors: Ngueko AMK; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.; University of Rome 'Tor Vergata', Rome, Italy., Ndjeyep SCD; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon., Semengue ENJ; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon., Nka AD; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon., Chenwi CA; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.; University of Rome 'Tor Vergata', Rome, Italy., Ka'e AC; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon., Bouba Y; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.; Faculty of Medicine, UniCamillus-Saint Camillus International University of Health Sciences, Rome, Italy., Gabisa JE; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon., Molimbou E; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.; University of Rome 'Tor Vergata', Rome, Italy., Etame NK; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.; University of Rome 'Tor Vergata', Rome, Italy., Tekoh TA; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon., Mundo RAN; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon., Tueguem PP; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon., Mekel VK; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.; University of Rome 'Tor Vergata', Rome, Italy., Mba H; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon., Takou D; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon., Beloumou GA; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.; University of Rome 'Tor Vergata', Rome, Italy., Tchouaket MCT; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon., Fotso LGM; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon., Ayuk DT; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon., Ouankou CN; Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, Dschang, Cameroon., Alteri C; Department of Oncology and Hemato-Oncology, University of Milan, Milan, Italy.; Microbiology and Virology Unit, Fondazione IRCCS Ca' Granda Policlinico, Milan, Italy., Colagrossi L; Multimodal Research Area, Microbiology and Diagnostics of Immunology Unit, IRCCS Bambino Gesu Paediatric Hospital, Rome, Italy., Boum Y 2nd; COVID-19 National Public Health Emergency Operations Coordination Centre, Yaounde, Cameroon., Ekane HGE; Faculty of Health Sciences, University of Buea, Buea, Cameroon., Ceccherini-Silberstein F; University of Rome 'Tor Vergata', Rome, Italy., Colizzi V; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.; Faculty of Medicine, 'Good Samaritan' Teaching Hospital, N'Djamena, Chad.; Evangelical University of Cameroon, Bandjoun, Cameroon., Ndembi N; International Vaccine Institute, Kigali, Rwanda.; Institute of Human Virology, University of Baltimore, Maryland, USA., Njamnshi AK; Brain Research Africa Initiative (BRAIN), Yaoundé, Cameroon.; Brain Research Africa Initiative (BRAIN), Geneva, Switzerland.; Neuroscience Lab, Faculty of Medicine and Biomedical Sciences, The University of YaoundéI, Yaoundé, Cameroon., Ndjolo A; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.; Neuroscience Lab, Faculty of Medicine and Biomedical Sciences, The University of YaoundéI, Yaoundé, Cameroon., Perno CF; Multimodal Research Area, Microbiology and Diagnostics of Immunology Unit, IRCCS Bambino Gesu Paediatric Hospital, Rome, Italy., Santoro MM; University of Rome 'Tor Vergata', Rome, Italy., Fokam J; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.; COVID-19 National Public Health Emergency Operations Coordination Centre, Yaounde, Cameroon.; Faculty of Health Sciences, University of Buea, Buea, Cameroon.; Evangelical University of Cameroon, Bandjoun, Cameroon.; National AIDS Control Committee (NACC), Yaoundé, Cameroon.
Source: Journal of clinical laboratory analysis [J Clin Lab Anal] 2026 Feb; Vol. 40 (4), pp. e70174. Date of Electronic Publication: 2026 Feb 01.
Publication Type: Journal Article; Comparative Study
Journal Info: Publisher: Wiley Country of Publication: United States NLM ID: 8801384 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-2825 (Electronic) Linking ISSN: 08878013 NLM ISO Abbreviation: J Clin Lab Anal Subsets: MEDLINE
Database: MEDLINE Ultimate
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PubType: Academic Journal
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  Data: Diagnostic Concordance of Two- and Three-Gene SARS-CoV-2 Molecular Assays in Cameroon: Implications for Efficient Pandemic Response in Low- and Middle-Income Countries.
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  Data: <searchLink fieldCode="AU" term="%22Ngueko+AMK%22">Ngueko AMK</searchLink>; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.; University of Rome 'Tor Vergata', Rome, Italy.<br /><searchLink fieldCode="AU" term="%22Ndjeyep+SCD%22">Ndjeyep SCD</searchLink>; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.<br /><searchLink fieldCode="AU" term="%22Semengue+ENJ%22">Semengue ENJ</searchLink>; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.<br /><searchLink fieldCode="AU" term="%22Nka+AD%22">Nka AD</searchLink>; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.<br /><searchLink fieldCode="AU" term="%22Chenwi+CA%22">Chenwi CA</searchLink>; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.; University of Rome 'Tor Vergata', Rome, Italy.<br /><searchLink fieldCode="AU" term="%22Ka'e+AC%22">Ka'e AC</searchLink>; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.<br /><searchLink fieldCode="AU" term="%22Bouba+Y%22">Bouba Y</searchLink>; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.; Faculty of Medicine, UniCamillus-Saint Camillus International University of Health Sciences, Rome, Italy.<br /><searchLink fieldCode="AU" term="%22Gabisa+JE%22">Gabisa JE</searchLink>; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.<br /><searchLink fieldCode="AU" term="%22Molimbou+E%22">Molimbou E</searchLink>; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.; University of Rome 'Tor Vergata', Rome, Italy.<br /><searchLink fieldCode="AU" term="%22Etame+NK%22">Etame NK</searchLink>; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.; University of Rome 'Tor Vergata', Rome, Italy.<br /><searchLink fieldCode="AU" term="%22Tekoh+TA%22">Tekoh TA</searchLink>; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.<br /><searchLink fieldCode="AU" term="%22Mundo+RAN%22">Mundo RAN</searchLink>; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.<br /><searchLink fieldCode="AU" term="%22Tueguem+PP%22">Tueguem PP</searchLink>; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.<br /><searchLink fieldCode="AU" term="%22Mekel+VK%22">Mekel VK</searchLink>; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.; University of Rome 'Tor Vergata', Rome, Italy.<br /><searchLink fieldCode="AU" term="%22Mba+H%22">Mba H</searchLink>; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.<br /><searchLink fieldCode="AU" term="%22Takou+D%22">Takou D</searchLink>; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.<br /><searchLink fieldCode="AU" term="%22Beloumou+GA%22">Beloumou GA</searchLink>; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.; University of Rome 'Tor Vergata', Rome, Italy.<br /><searchLink fieldCode="AU" term="%22Tchouaket+MCT%22">Tchouaket MCT</searchLink>; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.<br /><searchLink fieldCode="AU" term="%22Fotso+LGM%22">Fotso LGM</searchLink>; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.<br /><searchLink fieldCode="AU" term="%22Ayuk+DT%22">Ayuk DT</searchLink>; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.<br /><searchLink fieldCode="AU" term="%22Ouankou+CN%22">Ouankou CN</searchLink>; Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, Dschang, Cameroon.<br /><searchLink fieldCode="AU" term="%22Alteri+C%22">Alteri C</searchLink>; Department of Oncology and Hemato-Oncology, University of Milan, Milan, Italy.; Microbiology and Virology Unit, Fondazione IRCCS Ca' Granda Policlinico, Milan, Italy.<br /><searchLink fieldCode="AU" term="%22Colagrossi+L%22">Colagrossi L</searchLink>; Multimodal Research Area, Microbiology and Diagnostics of Immunology Unit, IRCCS Bambino Gesu Paediatric Hospital, Rome, Italy.<br /><searchLink fieldCode="AU" term="%22Boum+Y+2nd%22">Boum Y 2nd</searchLink>; COVID-19 National Public Health Emergency Operations Coordination Centre, Yaounde, Cameroon.<br /><searchLink fieldCode="AU" term="%22Ekane+HGE%22">Ekane HGE</searchLink>; Faculty of Health Sciences, University of Buea, Buea, Cameroon.<br /><searchLink fieldCode="AU" term="%22Ceccherini-Silberstein+F%22">Ceccherini-Silberstein F</searchLink>; University of Rome 'Tor Vergata', Rome, Italy.<br /><searchLink fieldCode="AU" term="%22Colizzi+V%22">Colizzi V</searchLink>; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.; Faculty of Medicine, 'Good Samaritan' Teaching Hospital, N'Djamena, Chad.; Evangelical University of Cameroon, Bandjoun, Cameroon.<br /><searchLink fieldCode="AU" term="%22Ndembi+N%22">Ndembi N</searchLink>; International Vaccine Institute, Kigali, Rwanda.; Institute of Human Virology, University of Baltimore, Maryland, USA.<br /><searchLink fieldCode="AU" term="%22Njamnshi+AK%22">Njamnshi AK</searchLink>; Brain Research Africa Initiative (BRAIN), Yaoundé, Cameroon.; Brain Research Africa Initiative (BRAIN), Geneva, Switzerland.; Neuroscience Lab, Faculty of Medicine and Biomedical Sciences, The University of YaoundéI, Yaoundé, Cameroon.<br /><searchLink fieldCode="AU" term="%22Ndjolo+A%22">Ndjolo A</searchLink>; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.; Neuroscience Lab, Faculty of Medicine and Biomedical Sciences, The University of YaoundéI, Yaoundé, Cameroon.<br /><searchLink fieldCode="AU" term="%22Perno+CF%22">Perno CF</searchLink>; Multimodal Research Area, Microbiology and Diagnostics of Immunology Unit, IRCCS Bambino Gesu Paediatric Hospital, Rome, Italy.<br /><searchLink fieldCode="AU" term="%22Santoro+MM%22">Santoro MM</searchLink>; University of Rome 'Tor Vergata', Rome, Italy.<br /><searchLink fieldCode="AU" term="%22Fokam+J%22">Fokam J</searchLink>; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.; COVID-19 National Public Health Emergency Operations Coordination Centre, Yaounde, Cameroon.; Faculty of Health Sciences, University of Buea, Buea, Cameroon.; Evangelical University of Cameroon, Bandjoun, Cameroon.; National AIDS Control Committee (NACC), Yaoundé, Cameroon.
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