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Comparison of culture and culture-free methods for comprehensive identification of mycobacteria: a single-center prospective study.

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Title: Comparison of culture and culture-free methods for comprehensive identification of mycobacteria: a single-center prospective study.
Authors: Hashimoto K; Department of Respiratory Medicine and Clinical Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan., Fukushima K; Department of Respiratory Medicine and Clinical Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.; Department of Infection Metagenomics, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan., Matsumoto Y; Department of Infection Metagenomics, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan., Saito H; Department of Clinical Laboratory, National Hospital Organization, Osaka Toneyama Medical Center, Toyonaka, Osaka, Japan., Funauchi A; Department of Respiratory Medicine and Clinical Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan., Hamada N; Laboratory of Host Defense, World Premier Institute Immunology Frontier Research Centre (WPI-IFReC), Osaka University, Osaka, Japan., Yamauchi J; Department of Clinical Laboratory, National Hospital Organization, Osaka Toneyama Medical Center, Toyonaka, Osaka, Japan., Nitta T; Department of Clinical Laboratory, National Hospital Organization, Osaka Toneyama Medical Center, Toyonaka, Osaka, Japan., Motooka D; Department of Infection Metagenomics, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan., Nii T; Department of Respiratory Medicine, National Hospital Organisation, Osaka Toneyama Medical Centre, Toyonaka, Osaka, Japan., Matsuki T; Department of Respiratory Medicine, National Hospital Organisation, Osaka Toneyama Medical Centre, Toyonaka, Osaka, Japan., Tsujino K; Department of Respiratory Medicine, National Hospital Organisation, Osaka Toneyama Medical Centre, Toyonaka, Osaka, Japan., Miki K; Department of Respiratory Medicine, National Hospital Organisation, Osaka Toneyama Medical Centre, Toyonaka, Osaka, Japan., Komukai S; Department of Health Data Science, Tokyo Medical University, Shinjuku, Tokyo, Japan., Kumanogoh A; Department of Respiratory Medicine and Clinical Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.; Integrated Frontier Research for Medical Science Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Suita, Japan.; Centre for Infectious Disease Education and Research, Osaka University, Suita, Japan., Nakamura S; Department of Infection Metagenomics, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan.; Integrated Frontier Research for Medical Science Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Suita, Japan.; Centre for Infectious Disease Education and Research, Osaka University, Suita, Japan., Kida H; Department of Respiratory Medicine, National Hospital Organisation, Osaka Toneyama Medical Centre, Toyonaka, Osaka, Japan.
Source: Journal of clinical microbiology [J Clin Microbiol] 2026 Apr 08; Vol. 64 (4), pp. e0112825. Date of Electronic Publication: 2026 Feb 27.
Publication Type: Journal Article; Comparative Study; Evaluation Study
Journal Info: Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7505564 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-660X (Electronic) Linking ISSN: 00951137 NLM ISO Abbreviation: J Clin Microbiol Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: Comparison of culture and culture-free methods for comprehensive identification of mycobacteria: a single-center prospective study.
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  Data: <searchLink fieldCode="AU" term="%22Hashimoto+K%22">Hashimoto K</searchLink>; Department of Respiratory Medicine and Clinical Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.<br /><searchLink fieldCode="AU" term="%22Fukushima+K%22">Fukushima K</searchLink>; Department of Respiratory Medicine and Clinical Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.; Department of Infection Metagenomics, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan.<br /><searchLink fieldCode="AU" term="%22Matsumoto+Y%22">Matsumoto Y</searchLink>; Department of Infection Metagenomics, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan.<br /><searchLink fieldCode="AU" term="%22Saito+H%22">Saito H</searchLink>; Department of Clinical Laboratory, National Hospital Organization, Osaka Toneyama Medical Center, Toyonaka, Osaka, Japan.<br /><searchLink fieldCode="AU" term="%22Funauchi+A%22">Funauchi A</searchLink>; Department of Respiratory Medicine and Clinical Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.<br /><searchLink fieldCode="AU" term="%22Hamada+N%22">Hamada N</searchLink>; Laboratory of Host Defense, World Premier Institute Immunology Frontier Research Centre (WPI-IFReC), Osaka University, Osaka, Japan.<br /><searchLink fieldCode="AU" term="%22Yamauchi+J%22">Yamauchi J</searchLink>; Department of Clinical Laboratory, National Hospital Organization, Osaka Toneyama Medical Center, Toyonaka, Osaka, Japan.<br /><searchLink fieldCode="AU" term="%22Nitta+T%22">Nitta T</searchLink>; Department of Clinical Laboratory, National Hospital Organization, Osaka Toneyama Medical Center, Toyonaka, Osaka, Japan.<br /><searchLink fieldCode="AU" term="%22Motooka+D%22">Motooka D</searchLink>; Department of Infection Metagenomics, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan.<br /><searchLink fieldCode="AU" term="%22Nii+T%22">Nii T</searchLink>; Department of Respiratory Medicine, National Hospital Organisation, Osaka Toneyama Medical Centre, Toyonaka, Osaka, Japan.<br /><searchLink fieldCode="AU" term="%22Matsuki+T%22">Matsuki T</searchLink>; Department of Respiratory Medicine, National Hospital Organisation, Osaka Toneyama Medical Centre, Toyonaka, Osaka, Japan.<br /><searchLink fieldCode="AU" term="%22Tsujino+K%22">Tsujino K</searchLink>; Department of Respiratory Medicine, National Hospital Organisation, Osaka Toneyama Medical Centre, Toyonaka, Osaka, Japan.<br /><searchLink fieldCode="AU" term="%22Miki+K%22">Miki K</searchLink>; Department of Respiratory Medicine, National Hospital Organisation, Osaka Toneyama Medical Centre, Toyonaka, Osaka, Japan.<br /><searchLink fieldCode="AU" term="%22Komukai+S%22">Komukai S</searchLink>; Department of Health Data Science, Tokyo Medical University, Shinjuku, Tokyo, Japan.<br /><searchLink fieldCode="AU" term="%22Kumanogoh+A%22">Kumanogoh A</searchLink>; Department of Respiratory Medicine and Clinical Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.; Integrated Frontier Research for Medical Science Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Suita, Japan.; Centre for Infectious Disease Education and Research, Osaka University, Suita, Japan.<br /><searchLink fieldCode="AU" term="%22Nakamura+S%22">Nakamura S</searchLink>; Department of Infection Metagenomics, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan.; Integrated Frontier Research for Medical Science Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Suita, Japan.; Centre for Infectious Disease Education and Research, Osaka University, Suita, Japan.<br /><searchLink fieldCode="AU" term="%22Kida+H%22">Kida H</searchLink>; Department of Respiratory Medicine, National Hospital Organisation, Osaka Toneyama Medical Centre, Toyonaka, Osaka, Japan.
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  Data: <searchLink fieldCode="JN" term="%227505564%22">Journal of clinical microbiology</searchLink> [J Clin Microbiol] 2026 Apr 08; Vol. 64 (4), pp. e0112825. <i>Date of Electronic Publication: </i>2026 Feb 27.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22American+Society+for+Microbiology%22">American Society for Microbiology </searchLink><i>Country of Publication: </i>United States <i>NLM ID: </i>7505564 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1098-660X (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2200951137%22">00951137 </searchLink><i>NLM ISO Abbreviation: </i>J Clin Microbiol <i>Subsets: </i>MEDLINE
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