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Development and validation of a propidium monoazide-qPCR assay with matrix-matched calibration for selective quantification of viable Limosilactobacillus reuteri in multi-strain probiotic powders.

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Title: Development and validation of a propidium monoazide-qPCR assay with matrix-matched calibration for selective quantification of viable Limosilactobacillus reuteri in multi-strain probiotic powders.
Authors: Lee J; Ildong Bioscience, Pyeongtaek-si, Gyeonggi-do 17957, Republic of Korea., Moon JS; Ildong Bioscience, Pyeongtaek-si, Gyeonggi-do 17957, Republic of Korea. Electronic address: moonjs@ildong.com.
Source: Journal of microbiological methods [J Microbiol Methods] 2026 May; Vol. 244, pp. 107467. Date of Electronic Publication: 2026 Mar 11.
Publication Type: Journal Article; Validation Study
Journal Info: Publisher: Elsevier Biomedical Country of Publication: Netherlands NLM ID: 8306883 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-8359 (Electronic) Linking ISSN: 01677012 NLM ISO Abbreviation: J Microbiol Methods Subsets: MEDLINE
Database: MEDLINE Ultimate
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PubType: Academic Journal
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  Data: Development and validation of a propidium monoazide-qPCR assay with matrix-matched calibration for selective quantification of viable Limosilactobacillus reuteri in multi-strain probiotic powders.
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  Data: <searchLink fieldCode="AU" term="%22Lee+J%22">Lee J</searchLink>; Ildong Bioscience, Pyeongtaek-si, Gyeonggi-do 17957, Republic of Korea.<br /><searchLink fieldCode="AU" term="%22Moon+JS%22">Moon JS</searchLink>; Ildong Bioscience, Pyeongtaek-si, Gyeonggi-do 17957, Republic of Korea. Electronic address: moonjs@ildong.com.
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  Data: <searchLink fieldCode="JN" term="%228306883%22">Journal of microbiological methods</searchLink> [J Microbiol Methods] 2026 May; Vol. 244, pp. 107467. <i>Date of Electronic Publication: </i>2026 Mar 11.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Elsevier+Biomedical%22">Elsevier Biomedical </searchLink><i>Country of Publication: </i>Netherlands <i>NLM ID: </i>8306883 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1872-8359 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2201677012%22">01677012 </searchLink><i>NLM ISO Abbreviation: </i>J Microbiol Methods <i>Subsets: </i>MEDLINE
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        Value: 10.1016/j.mimet.2026.107467
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      – Code: eng
        Text: English
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        StartPage: 107467
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      – TitleFull: Development and validation of a propidium monoazide-qPCR assay with matrix-matched calibration for selective quantification of viable Limosilactobacillus reuteri in multi-strain probiotic powders.
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            NameFull: Lee J
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            NameFull: Moon JS
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            – D: 01
              M: 05
              Text: 2026 May
              Type: published
              Y: 2026
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              Value: 1872-8359
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              Value: 244
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            – TitleFull: Journal of microbiological methods
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