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A rapid and highly sensitive CRISPR-Cas12a ortholog-assisted assay for genotyping of myostatin knockout pigs.

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Title: A rapid and highly sensitive CRISPR-Cas12a ortholog-assisted assay for genotyping of myostatin knockout pigs.
Authors: Wang Y; College of Animal Science and Veterinary Medicine, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China.; Hubei Hongshan Laboratory, Wuhan, Hubei, 430070, People's Republic of China.; Yazhouwan National Laboratory, Sanya, 572024, People's Republic of China., Yang H; College of Animal Science and Veterinary Medicine, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China.; Hubei Hongshan Laboratory, Wuhan, Hubei, 430070, People's Republic of China., Zhang W; College of Animal Science and Veterinary Medicine, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China.; Yazhouwan National Laboratory, Sanya, 572024, People's Republic of China., Tao D; College of Animal Science and Veterinary Medicine, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China., Shi S; College of Animal Science and Veterinary Medicine, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China., Li S; College of Animal Science and Veterinary Medicine, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China., Wu X; Shanghai Key Laboratory of Agricultural Genetics and Breeding Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, People's Republic of China., Ma Y; College of Animal Science and Veterinary Medicine, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China., Ruan J; College of Animal Science and Veterinary Medicine, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China., Jing L; New Hope Liuhe Co., Ltd. Key Laboratory of Digital Intelligent Breeding Technological Innovation for Swine and Poultry, Ministry of Agriculture and Rural Affairs, Chengdu, Sichuan, 610023, People's Republic of China., Ma K; New Hope Liuhe Co., Ltd. Key Laboratory of Digital Intelligent Breeding Technological Innovation for Swine and Poultry, Ministry of Agriculture and Rural Affairs, Chengdu, Sichuan, 610023, People's Republic of China., Li X; College of Animal Science and Veterinary Medicine, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China.; Hubei Hongshan Laboratory, Wuhan, Hubei, 430070, People's Republic of China., Han X; College of Animal Science and Veterinary Medicine, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China. xshan@mail.hzau.edu.cn.; Yazhouwan National Laboratory, Sanya, 572024, People's Republic of China. xshan@mail.hzau.edu.cn., Xu X; College of Animal Science and Veterinary Medicine, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China. xuewen_xu@mail.hzau.edu.cn., Xie S; College of Animal Science and Veterinary Medicine, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China. ssxie@mail.hzau.edu.cn.; Hubei Hongshan Laboratory, Wuhan, Hubei, 430070, People's Republic of China. ssxie@mail.hzau.edu.cn.
Source: Cell & bioscience [Cell Biosci] 2026 Mar 29; Vol. 16 (1). Date of Electronic Publication: 2026 Mar 29.
Publication Type: Journal Article
Journal Info: Publisher: BioMed Central Country of Publication: England NLM ID: 101561195 Publication Model: Electronic Cited Medium: Print ISSN: 2045-3701 (Print) Linking ISSN: 20453701 NLM ISO Abbreviation: Cell Biosci Subsets: PubMed not MEDLINE
Database: MEDLINE Ultimate
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  Data: A rapid and highly sensitive CRISPR-Cas12a ortholog-assisted assay for genotyping of myostatin knockout pigs.
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  Data: <searchLink fieldCode="AU" term="%22Wang+Y%22">Wang Y</searchLink>; College of Animal Science and Veterinary Medicine, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China.; Hubei Hongshan Laboratory, Wuhan, Hubei, 430070, People's Republic of China.; Yazhouwan National Laboratory, Sanya, 572024, People's Republic of China.<br /><searchLink fieldCode="AU" term="%22Yang+H%22">Yang H</searchLink>; College of Animal Science and Veterinary Medicine, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China.; Hubei Hongshan Laboratory, Wuhan, Hubei, 430070, People's Republic of China.<br /><searchLink fieldCode="AU" term="%22Zhang+W%22">Zhang W</searchLink>; College of Animal Science and Veterinary Medicine, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China.; Yazhouwan National Laboratory, Sanya, 572024, People's Republic of China.<br /><searchLink fieldCode="AU" term="%22Tao+D%22">Tao D</searchLink>; College of Animal Science and Veterinary Medicine, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China.<br /><searchLink fieldCode="AU" term="%22Shi+S%22">Shi S</searchLink>; College of Animal Science and Veterinary Medicine, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China.<br /><searchLink fieldCode="AU" term="%22Li+S%22">Li S</searchLink>; College of Animal Science and Veterinary Medicine, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China.<br /><searchLink fieldCode="AU" term="%22Wu+X%22">Wu X</searchLink>; Shanghai Key Laboratory of Agricultural Genetics and Breeding Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, People's Republic of China.<br /><searchLink fieldCode="AU" term="%22Ma+Y%22">Ma Y</searchLink>; College of Animal Science and Veterinary Medicine, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China.<br /><searchLink fieldCode="AU" term="%22Ruan+J%22">Ruan J</searchLink>; College of Animal Science and Veterinary Medicine, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China.<br /><searchLink fieldCode="AU" term="%22Jing+L%22">Jing L</searchLink>; New Hope Liuhe Co., Ltd. Key Laboratory of Digital Intelligent Breeding Technological Innovation for Swine and Poultry, Ministry of Agriculture and Rural Affairs, Chengdu, Sichuan, 610023, People's Republic of China.<br /><searchLink fieldCode="AU" term="%22Ma+K%22">Ma K</searchLink>; New Hope Liuhe Co., Ltd. Key Laboratory of Digital Intelligent Breeding Technological Innovation for Swine and Poultry, Ministry of Agriculture and Rural Affairs, Chengdu, Sichuan, 610023, People's Republic of China.<br /><searchLink fieldCode="AU" term="%22Li+X%22">Li X</searchLink>; College of Animal Science and Veterinary Medicine, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China.; Hubei Hongshan Laboratory, Wuhan, Hubei, 430070, People's Republic of China.<br /><searchLink fieldCode="AU" term="%22Han+X%22">Han X</searchLink>; College of Animal Science and Veterinary Medicine, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China. xshan@mail.hzau.edu.cn.; Yazhouwan National Laboratory, Sanya, 572024, People's Republic of China. xshan@mail.hzau.edu.cn.<br /><searchLink fieldCode="AU" term="%22Xu+X%22">Xu X</searchLink>; College of Animal Science and Veterinary Medicine, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China. xuewen_xu@mail.hzau.edu.cn.<br /><searchLink fieldCode="AU" term="%22Xie+S%22">Xie S</searchLink>; College of Animal Science and Veterinary Medicine, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China. ssxie@mail.hzau.edu.cn.; Hubei Hongshan Laboratory, Wuhan, Hubei, 430070, People's Republic of China. ssxie@mail.hzau.edu.cn.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22BioMed+Central%22">BioMed Central </searchLink><i>Country of Publication: </i>England <i>NLM ID: </i>101561195 <i>Publication Model: </i>Electronic <i>Cited Medium: </i>Print <i>ISSN: </i>2045-3701 (Print) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2220453701%22">20453701 </searchLink><i>NLM ISO Abbreviation: </i>Cell Biosci <i>Subsets: </i>PubMed not MEDLINE
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        Value: 10.1186/s13578-026-01559-2
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