Next generation sequencing approaches for the detection and characterization of enteroviruses in clinical, public health, and research settings: Expert view of the European non-polio enterovirus network (ENPEN).

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Title: Next generation sequencing approaches for the detection and characterization of enteroviruses in clinical, public health, and research settings: Expert view of the European non-polio enterovirus network (ENPEN).
Authors: Benschop KSM; Centre for Infectious Disease Control (CIb), Centre for Infectious Disease Research, Diagnostics and laboratory Surveillance (IDS) Dutch National Institute for Public Health and the Environment, Bilthoven, the Netherlands. Electronic address: kim.benschop@rivm.nl., Zwagemaker F; Centre for Infectious Disease Control (CIb), Centre for Infectious Disease Research, Diagnostics and laboratory Surveillance (IDS) Dutch National Institute for Public Health and the Environment, Bilthoven, the Netherlands., Andersson-Li L; Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Sweden., Andrés C; Respiratory Viruses Unit, Microbiology Department, Vall d'Hebron Hospital Universitari, Vall d'Hebron Institut de Recerca (VHIR), Vall d'Hebron Barcelona Hospital Campus, Spain., Antón A; Respiratory Viruses Unit, Microbiology Department, Vall d'Hebron Hospital Universitari, Vall d'Hebron Institut de Recerca (VHIR), Vall d'Hebron Barcelona Hospital Campus, Spain., Berengua C; Microbiology Department. Hospital Universitari de la Santa Creu i Sant Pau, Universitat Autònoma de Barcelona, Barcelona, Spain., Berginc N; National Laboratory of Health, Environment and Food, Laboratory for Public Health Virology, Ljubljana, Slovenia., Bessaud M; Institut Pasteur, Université de Paris Cité, CNRS UMR 3569, Virus sensing & signaling Unit, Paris, France., Bisseux M; CHU Clermont-Ferrand, 3 IHP, National Reference Centre for enteroviruses and parechoviruses, Clermont-Ferrand, France., Bujaki E; Division of Vaccines, Science and Research, Medicines and Healthcare products Regulatory Agency, South Mimms, (MHRA), Hertfordshire EN6 3QG, United Kingdom., Canning B; Clinical Microbiology, Nottingham University Hospitals NHS Trust, Nottingham, NG7 2UH, United Kingdom., Christiansen CB; Department of Clinical microbiology, Lund University Hospital, Lund, Sweden., Couderé K; ElisabethTweesteden Hospital, Tilburg, the Netherlands; Zorgsaam, Terneuzen, the Netherlands., Broberg EK; European Centre for Disease Prevention and Control, (ECDC), Stockholm, Sweden., Cassidy H; Viroscience, Erasmus Medical Center, Rotterdam, the Netherlands., Castilletti C; Department of Infectious, Tropical Diseases and Microbiology - IRCCS Sacro Cuore Don Calabria Hospital, Negrar di Valpolicella (VR), Italy., Celma C; Enteric Virus Unit, Virus Reference Department, UK Health Security Agency, London, United Kingdom., Cinek O; Department of Microbiology, Second Faculty of Medicine, Charles University, and University Hospital Motol, Prague, Czech Republic., Deézsi-Magyar N; National Biosafety Laboratory, National Center for Public Health and Pharmacy, Budapest, Hungary., Eis-Hübinger AM; Institute of Virology, University Hospital Bonn, Germany., Flipse J; Laboratory for Medical Microbiology and Immunology, Dicoon, Elst, the Netherlands., Jiřincová H; National Reference Laboratory for Enteroviruses, National Institute of Public Health, Prague, Czech Republic., Gatej R; National Institute of Public Health, Bucharest, Romania., Georgieva I; National Reference Laboratory for Enteroviruses, Department of Virology, National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria., Giardina F; Department of Clinical Surgical Diagnostic and Pediatric Sciences, University of Pavia, Pavia, Italy., González-Sánchez A; Respiratory Viruses Unit, Microbiology Department, Vall d'Hebron Hospital Universitari, Vall d'Hebron Institut de Recerca (VHIR), Vall d'Hebron Barcelona Hospital Campus, Spain., Hack B; Infectious Diseases and Microbiology Department, Guy's and St Thomas' NHS Foundation Trust, United Kingdom., Helfferich J; Department of Neurology, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands., Hutchings S; Virology laboratory, Bristol, United Kingdom Health Security Agency, Bristol, United Kingdom., Hietanen E; Astbury Centre for Structural Molecular Biology, School of Molecular & Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, United Kingdom., Hönemann M; Institute of Medical Microbiology and Virology, University of Leipzig, Leipzig, Germany., Virant MJ; Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Slovenia., Kalogera E; Microbiology Department, Agia Sofia Children's Hospital, Athens, Greece., Johannesen CK; Department of Clinical Research, Nordsjællands Hospital, Hilleroed, Denmark., Kenicer J; Pathogen Sequencing Service, Specialist Virology Centre, Edinburgh, Royal Infirmary of Edinburgh, Edinbrugh, Scotland, United Kingdom., Kleines M; Laboratory Diagnostic Center, University Hospital RWTH Aachen, Aachen, Germany., Lagarejos E; Microbiology Department, Doctor Negrin Gran Canaria's Universitary Hospital, Gran Canaria, Spain., Landaas ET; Department of Microbiology, Oslo University Hospital, Oslo, Norway; Institute of Clinical Medicine, University of Oslo, Oslo, Norway., Kandulu CC; Pharmaceutical Care Research Group, University College Cork, Cork, Ireland., Xavier López Labrador F; CIBERESP, Instituto de Salud Carlos III, Madrid, Spain; Virology Laboratory, Genomics and Health Area, Center for Public Health Research (FISABIO - Public Health), Valencia, Spain; Department of Microbiology & Ecology, University of Valencia Medical School, Spain., Lunar MM; Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Slovenia., Maier M; Institute of Medical Microbiology and Virology, University of Leipzig, Leipzig, Germany., Majumdar M; Division of Vaccines, Science and Research, Medicines and Healthcare products Regulatory Agency, South Mimms, (MHRA), Hertfordshire EN6 3QG, United Kingdom., Martin J; Division of Vaccines, Science and Research, Medicines and Healthcare products Regulatory Agency, South Mimms, (MHRA), Hertfordshire EN6 3QG, United Kingdom., McClure CP; Wolfson Centre for Global Virus Research, School of Life Sciences, University of Nottingham, Nottingham, United Kingdom., Muñoz-Almagro C; Grupo de Investigación en Enfermedades Infecciosas y Microbioma, Institut de Recerca Sant Joan de Déu,Institut Recerca Sant Joan de Deu-Hospital Sant Joan de Deu, CIBER de Epidemiología y Salud Pública- CIBERESP, Medicine Department, Universitat Internacional de Catalunya, Spain., Ošep A; Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Slovenia., Øverbø J; Department of Virology, Norwegian Institute of Public Health, Norway., Palminha P; National Reference Laboratory for Influenza and Other Respiratory Viruses, National Institute of Health Dr Ricardo Jorge (INSA), Lisbon, Portugal., Papa A; Department of Microbiology, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece., Pariani E; Department of Biomedical Sciences for Health, University of Milan, Milano (MI), Italy., Pellegrinelli L; Department of Biomedical Sciences for Health, University of Milan, Milano (MI), Italy., Pietsch C; Institute of Medical Microbiology and Virology, University of Leipzig, Leipzig, Germany., Piralla A; Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy., Poljak M; Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Slovenia., Pomari E; Department of Infectious, Tropical Diseases and Microbiology - IRCCS Sacro Cuore Don Calabria Hospital, Negrar di Valpolicella (VR), Italy., Prats-Méndez I; Respiratory Viruses Unit, Microbiology Department, Vall d'Hebron Hospital Universitari, Vall d'Hebron Institut de Recerca (VHIR), Vall d'Hebron Barcelona Hospital Campus, Spain., Rector A; Department of Microbiology, immunology and Transplantation, Laboratory of Clinical and Epidemiological Virology, Reference center for Enterovirus, KU Leuven, Rega Institute, Belgium., Reuter G; Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Hungary., Riess M; European Centre for Disease Prevention and Control, (ECDC), Stockholm, Sweden., Ruta S; Carol Davila University of Medicine and Pharmacy, Bucharest, Romania; Stefan S Nicolau Institute of Virology, Bucharest, Romania., Schibler M; Laboratory of Virology, Division of Laboratory Medicine, Division of Infectious Diseases, Geneva University Hospitals, Switzerland., Nilsen HS; Department of Medical Microbiology, St. Olavs Hospital, Trondheim University Hospital, Norway., Simmonds P; Nuttfield Department for Medicine, University of Oxford, Oxford, United Kingdom., Sourvinos G; Laboratory of Clinical Virology, Medical School, University of Crete, Crete, Greece., Szomor K; National WHO Poliovirus Reference Laboratory, National Center for Public Health and Pharmacy, Budapest, Hungary., Susi P; Institute of Biomedicine, University of Turku, Finland., Tabain I; Microbiology Division, Croatian Institute of Public Health, Zagreb, Croatia., Vallely P; Microbiology and Virology Unit, Division of Evolution, Infection and Genomic Sciences, School of Biological Sciences, University of Manchester, Manchestar, United Kingdom., von Eije KJ; Viroscience, Erasmus Medical Center, Rotterdam, the Netherlands., Weil M; Central Virology Laboratory, Public Health Services, Ministry of Health, Chaim Sheba Medical Center, Ramat Gan, Israel., Wieczorek M; Department of Virology, National Institute of Public Health NIH - National Institute of Research, Warsaw, Poland., Wollants E; Department of Microbiology, immunology and Transplantation, Laboratory of Clinical and Epidemiological Virology, Reference center for Enterovirus, KU Leuven, Rega Institute, Belgium., Wolthers KC; OrganoVIR Labs, Dept of Medical Microbiology and Infection prevention, Amsterdam UMC location AMC, Amsterdam, the Netherlands., Zuckerman NS; Central Virology Laboratory, Public Health Services, Ministry of Health, Chaim Sheba Medical Center, Ramat Gan, Israel., Fischer TK; Department of Clinical Research, Nordsjællands Hospital, Hilleroed, Denmark; Department of Public Health, University of Copenhagen, Copenhagen, Denmark; Pandemix Center for Excellence (DNRF 170), Roskilde University, Roskilde, Denmark., Harvala H; Nuttfield Department for Medicine, University of Oxford, Oxford, United Kingdom; NHS Blood and Transplant, Microbiology Services, Colindale, United Kingdom; Clinical Microbiology, Turku University Hospital and Institute of Biomedicine, University of Turku, Turku, Finland.
Source: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology [J Clin Virol] 2026 Jun; Vol. 184, pp. 105940. Date of Electronic Publication: 2026 Mar 24.
Publication Type: Journal Article; Review
Journal Info: Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 9815671 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-5967 (Electronic) Linking ISSN: 13866532 NLM ISO Abbreviation: J Clin Virol Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:1873-5967
DOI:10.1016/j.jcv.2026.105940