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In-Situ ssDNA Isolation from dsDNA Sources as a Streamlined Pathway to DNA Origami Assembly and Testing.

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Title: In-Situ ssDNA Isolation from dsDNA Sources as a Streamlined Pathway to DNA Origami Assembly and Testing.
Authors: Ruiz EO; Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA.; University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA., Neyra K; Department of Chemistry, Case Western Reserve University, Cleveland, OH 44106, USA., Lopez DM; Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA.; Department of Biochemistry and Molecular Biology and Center for Mechanical Excitability, The University of Chicago, Chicago, IL, 60637, USA.; Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, 43210, USA., Chen RW; Ohio State Biochemistry Program, The Ohio State University, Columbus, Ohio, 43212, USA., Paramasamy D; Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA.; Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, AZ, 85281, USA., Bizjak Q; Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA., Halley PD; Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA., Wei Y; Biophysics Graduate Program, The Ohio State University, Columbus, OH 43210, USA., Sotomayor M; Department of Biochemistry and Molecular Biology and Center for Mechanical Excitability, The University of Chicago, Chicago, IL, 60637, USA., Poirier MG; Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, 43210, USA.; Ohio State Biochemistry Program, The Ohio State University, Columbus, Ohio, 43212, USA.; Biophysics Graduate Program, The Ohio State University, Columbus, OH 43210, USA.; Department of Physics, The Ohio State University, Columbus, OH 43210, USA., Mathur D; Department of Chemistry, Case Western Reserve University, Cleveland, OH 44106, USA., Castro CE; Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA.; Biophysics Graduate Program, The Ohio State University, Columbus, OH 43210, USA., Pfeifer WG; Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA.; Department of Biochemistry and Molecular Biology and Center for Mechanical Excitability, The University of Chicago, Chicago, IL, 60637, USA.; Department of Physics, The Ohio State University, Columbus, OH 43210, USA.
Source: BioRxiv : the preprint server for biology [bioRxiv] 2026 Mar 23. Date of Electronic Publication: 2026 Mar 23.
Publication Type: Journal Article; Preprint
Journal Info: Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet ISSN: 2692-8205 (Electronic) Linking ISSN: 26928205 NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE
Database: MEDLINE Ultimate
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  Data: <searchLink fieldCode="AU" term="%22Ruiz+EO%22">Ruiz EO</searchLink>; Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA.; University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA.<br /><searchLink fieldCode="AU" term="%22Neyra+K%22">Neyra K</searchLink>; Department of Chemistry, Case Western Reserve University, Cleveland, OH 44106, USA.<br /><searchLink fieldCode="AU" term="%22Lopez+DM%22">Lopez DM</searchLink>; Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA.; Department of Biochemistry and Molecular Biology and Center for Mechanical Excitability, The University of Chicago, Chicago, IL, 60637, USA.; Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, 43210, USA.<br /><searchLink fieldCode="AU" term="%22Chen+RW%22">Chen RW</searchLink>; Ohio State Biochemistry Program, The Ohio State University, Columbus, Ohio, 43212, USA.<br /><searchLink fieldCode="AU" term="%22Paramasamy+D%22">Paramasamy D</searchLink>; Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA.; Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, AZ, 85281, USA.<br /><searchLink fieldCode="AU" term="%22Bizjak+Q%22">Bizjak Q</searchLink>; Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA.<br /><searchLink fieldCode="AU" term="%22Halley+PD%22">Halley PD</searchLink>; Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA.<br /><searchLink fieldCode="AU" term="%22Wei+Y%22">Wei Y</searchLink>; Biophysics Graduate Program, The Ohio State University, Columbus, OH 43210, USA.<br /><searchLink fieldCode="AU" term="%22Sotomayor+M%22">Sotomayor M</searchLink>; Department of Biochemistry and Molecular Biology and Center for Mechanical Excitability, The University of Chicago, Chicago, IL, 60637, USA.<br /><searchLink fieldCode="AU" term="%22Poirier+MG%22">Poirier MG</searchLink>; Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, 43210, USA.; Ohio State Biochemistry Program, The Ohio State University, Columbus, Ohio, 43212, USA.; Biophysics Graduate Program, The Ohio State University, Columbus, OH 43210, USA.; Department of Physics, The Ohio State University, Columbus, OH 43210, USA.<br /><searchLink fieldCode="AU" term="%22Mathur+D%22">Mathur D</searchLink>; Department of Chemistry, Case Western Reserve University, Cleveland, OH 44106, USA.<br /><searchLink fieldCode="AU" term="%22Castro+CE%22">Castro CE</searchLink>; Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA.; Biophysics Graduate Program, The Ohio State University, Columbus, OH 43210, USA.<br /><searchLink fieldCode="AU" term="%22Pfeifer+WG%22">Pfeifer WG</searchLink>; Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA.; Department of Biochemistry and Molecular Biology and Center for Mechanical Excitability, The University of Chicago, Chicago, IL, 60637, USA.; Department of Physics, The Ohio State University, Columbus, OH 43210, USA.
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